FastQCFastQC Report
Fri 17 Jun 2016
SRR1527157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423899
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26540.6260925361937631No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG25380.5987275270760252No Hit
TATGTAGGGGTGGGGGGTGTCTCCTGTATTCTTTTAGTTGATGATGCTGTTA24380.5751370019745269No Hit
TATGTAGGGATGTGTGTCTGTCTGTATGGTGTATGTCTGTCTGTCTGGTGTG20010.4720464072809797No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17220.4062288422477996No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA16430.387592327417616No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA15320.3614068445549529No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12140.28638897473218855No Hit
TATGTAGGGATTTATAAGTTCTGAGATTGTGCTGTGAGTTTTTTTTTCACAG12000.28308630121797884No Hit
TATGTAGGGCTTTGGGGGGCTGCCACTGGCTGTTGCGCCTTGTGCCGGCAGC11480.2708192281651997No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAGTGTATACATGTCAGCATGTGT11430.2696397019101248No Hit
TATGTAGGGCTAAAGATGAGGCAGAAAATGGTTGAATCCCCTGTTTTTTTAT11370.2682242704040349No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA11310.266808838897945No Hit
TATGTAGGGATTTGTGAGATGACAGCCTTTTTGTATCTCAAACTGGATGCTT11020.25996758661851055No Hit
TATGTAGGGATGCAGATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCATGA10760.253834050092121No Hit
TATGTAGGGAGTCTTGATAGCCTCTGGAAAGCCCTGGCCTGTCGTGTTCCTT10000.23590525101498233No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA9930.23425391425787745No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9760.23024352499062276No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC8990.2120788206624691No Hit
TATGTAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA8810.20783252614419945No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA8730.2059452841360796No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA8660.20429394737897472No Hit
TATGTAGGGATCCTCACAGGAGTGAAAGGGAAAGAGCTCTGGTCTCTCTTCT8470.19981174760969003No Hit
TATGTAGGGATTTTGGATAGTGTGTCTTGGTACTAATAACTAAATGGATACA8050.18990372706706077No Hit
TATGTAGGGGTGTGCAGCTGAGGGAGAATTTCTCGCTTTCCTGTTGTGGCCA7940.187308769305896No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC7650.1804675170264615No Hit
TATGTAGGGAGCTCTGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTG7430.17527760150413188No Hit
TATGTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG7360.173626264747027No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA7040.16607729671454757No Hit
TATGTAGGGGATTGTTTTCCCCCTCCCAGGGAAATCTATGTACCCTCCCAGC7000.16513367571048765No Hit
TATGTAGGGATAGGCCCTGTTCATGTTTCTTCCTCTCATAGACATGCCCTTC6480.15286660265770854No Hit
TATGTAGGGATTCCCACCACTCCAAATTCTTCCTTGCCTACTTTCTCTCTTC5710.1347018983295549No Hit
TATGTAGGGGGGCTCGCGGACAGCTCCGCGGCCATCTGATTCTTTCGGTTTT5670.13375827732549497No Hit
TATGTAGGGATTCCAGCCCCAGGGCCTTTGCACTTGCTGTTTTTGTTGGCTG5640.13305056157245004No Hit
TATGTAGGGAGACTGTGGCTTCCTTAAGAACCGAGCTTTAGGACAGCAAGTG5580.13163513006636016No Hit
TATGTAGGGACGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAA5280.12455797253591067No Hit
TATGTAGGGATGTGTAAGGGTGTTTTGCAGGCATGTATACTATGTGGGTGTC5190.12243482527677585No Hit
TATGTAGGGATTTAGGGTGCATCTTCTTTCCTCAATTAAACCCTGTACAAAC5070.11960396226459605No Hit
TATGTAGGGATTTCAGGCTGTCATGATGTTGGCCTTCGCGATGCCTTCGCTA5060.11936805701358107No Hit
TATGTAGGGAGCCCTAGCTGCCCTGGAACTTACTTTATAGACCAGGCTAGCC4540.10710098396080198No Hit
TATGTAGGGGCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGA4450.10497783670166715No Hit
TATGTAGGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA4410.10403421569760722No Hit
TATGTAGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA4360.1028546894425323No Hit
TATGTAGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA4350.10261878419151732No Hit
TATGTAGGGATGGTACACTGGGTCTCCAGGCTAGCTCCGACATGGTACACTG4330.10214697368948736No Hit
TATGTAGGGAGGGATTGAACTTAGGGCTTGCAAAGTACAGACAAGCACAGTA4280.10096744743441244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAT253.4134813E-546.020
TGATCCG206.3069246E-446.035
TCGCCTA206.3069246E-446.019
ATCCTTG206.3069246E-446.037
CTTCGTC206.3069246E-446.023
TCGCCAC206.3069246E-446.030
ATCCTGC253.4134813E-546.026
GTCATAC206.3069246E-446.044
TTTAGAA206.3069246E-446.025
AGACTTA253.4134813E-546.046
GTGCAAG405.5970304E-946.020
GAAATCA206.3069246E-446.029
TGTCAAT206.3069246E-446.023
TTAGGAC405.5970304E-946.038
TCGTTCT253.4134813E-546.012
GTCGCGT253.4134813E-546.010
AGATCTT253.4134813E-546.044
TGCCCGT301.8586034E-646.046
CGAACCT453.092282E-1046.038
ACCGGAC206.3069246E-446.040
Produced by FastQC (version 0.11.5)