##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423899
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714004987037008	33.0	31.0	34.0	28.0	34.0
2	31.455761867803414	31.0	31.0	34.0	28.0	34.0
3	32.302050724347076	34.0	31.0	34.0	30.0	34.0
4	36.03266580010804	37.0	35.0	37.0	35.0	37.0
5	35.09982802507201	35.0	35.0	37.0	33.0	37.0
6	35.81192925673333	37.0	35.0	37.0	35.0	37.0
7	36.34190219840104	37.0	35.0	37.0	35.0	37.0
8	36.563955093076416	37.0	37.0	37.0	35.0	37.0
9	38.516665526457956	39.0	39.0	39.0	37.0	39.0
10	37.733323268042625	39.0	38.0	39.0	35.0	39.0
11	37.3071934588192	39.0	37.0	39.0	34.0	39.0
12	37.116140873179695	39.0	37.0	39.0	34.0	39.0
13	36.862212460987166	39.0	37.0	39.0	33.0	39.0
14	37.74221453695338	40.0	37.0	40.0	33.0	40.0
15	37.82802507201008	40.0	37.0	40.0	33.0	40.0
16	37.87512591442773	40.0	38.0	40.0	34.0	40.0
17	37.90488064373825	40.0	38.0	40.0	34.0	40.0
18	37.80804861535413	40.0	38.0	40.0	33.0	40.0
19	37.78108700421563	40.0	37.0	40.0	33.0	40.0
20	37.898857982679836	40.0	38.0	40.0	33.0	40.0
21	33.71671082026615	39.0	34.0	40.0	11.0	40.0
22	34.580600567588036	39.0	34.0	40.0	18.0	40.0
23	36.49820829961854	39.0	34.0	40.0	30.0	40.0
24	37.15044621478229	39.0	36.0	40.0	32.0	40.0
25	37.296122425389065	39.0	37.0	40.0	33.0	40.0
26	37.10043666061963	39.0	36.0	40.0	33.0	40.0
27	37.134520251286276	39.0	35.0	40.0	33.0	40.0
28	37.061189104008264	39.0	35.0	40.0	32.0	40.0
29	37.07840075112232	39.0	35.0	40.0	33.0	40.0
30	36.86348634934265	39.0	35.0	40.0	32.0	40.0
31	36.684288002566646	39.0	35.0	40.0	31.0	40.0
32	36.34985928251777	39.0	35.0	40.0	30.0	40.0
33	36.11410501086344	39.0	35.0	40.0	30.0	40.0
34	35.927808275084395	39.0	35.0	40.0	30.0	40.0
35	36.01951644141647	39.0	35.0	40.0	30.0	40.0
36	35.71375728652344	39.0	35.0	40.0	29.0	40.0
37	35.43060257278267	39.0	35.0	40.0	25.0	40.0
38	35.22441194718553	39.0	35.0	40.0	25.0	40.0
39	34.989877305678945	38.0	35.0	40.0	24.0	40.0
40	30.31050556854345	37.0	23.0	40.0	8.0	40.0
41	31.215607963217654	37.0	23.0	40.0	12.0	40.0
42	32.80196933703547	36.0	30.0	40.0	15.0	40.0
43	33.39764425016337	37.0	33.0	40.0	17.0	40.0
44	33.965156794425084	37.0	34.0	40.0	18.0	40.0
45	34.02980898751825	38.0	34.0	40.0	18.0	40.0
46	33.976433065423606	37.0	34.0	40.0	18.0	40.0
47	33.84122632985687	37.0	34.0	40.0	17.0	40.0
48	33.64505931837537	37.0	33.0	40.0	15.0	40.0
49	33.3618597826369	36.0	33.0	40.0	15.0	40.0
50	33.197072887645405	36.0	33.0	40.0	13.0	40.0
51	32.95366584964815	35.0	33.0	40.0	12.0	40.0
52	32.48515802113239	35.0	33.0	40.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	1.0
14	2.0
15	20.0
16	50.0
17	129.0
18	308.0
19	532.0
20	766.0
21	1233.0
22	1713.0
23	2428.0
24	3341.0
25	4589.0
26	6171.0
27	8351.0
28	10135.0
29	10376.0
30	10001.0
31	11113.0
32	14247.0
33	17445.0
34	28381.0
35	41336.0
36	53333.0
37	62499.0
38	93641.0
39	41754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.60014059952961	0.23095124074366769	0.1351737088315849	0.03373445089514247
8	98.90988183505976	0.86978266049224	0.11559357299734134	0.10474193145065216
9	95.97333327042527	2.508380534042307	0.6836534174414188	0.8346327780910076
10	42.08502497057082	42.50635174888358	5.529147273289156	9.879476007256445
11	28.275603386655785	33.96988433565543	23.81439918471145	13.940113092977334
12	36.318321109509576	24.961606420397313	22.327488387564017	16.392584082529094
13	23.9757583764057	26.21898140830717	26.083099983722533	23.722160231564597
14	27.157884307346798	33.10293253817537	21.203635771728642	18.535547382749193
15	27.33127466684281	28.78397920259307	25.84365615394233	18.04108997662179
16	28.594311380777025	32.375636649296176	24.867008414740305	14.163043555186494
17	30.305568071639705	27.59171406396335	23.46809027622146	18.634627588175483
18	28.959964519850246	29.265461819914652	23.66459935031694	18.109974309918165
19	26.514334782577926	27.82950655698645	28.540760888796623	17.115397771639
20	25.919617644769154	28.459373577196455	25.888006341133146	19.73300243690124
21	28.161897055666564	25.274416783243183	29.713209986341088	16.850476174749176
22	22.524233366910515	28.749065225442855	24.470451687784117	24.256249719862513
23	22.523997461659498	31.94345822943673	27.73655988808655	17.795984420817224
24	23.724283378823728	30.882592315622354	28.94533839428732	16.4477859112666
25	26.064463468892352	30.253904821667426	24.56292654618199	19.11870516325823
26	26.386474136527806	29.978839298983956	25.037096100722106	18.597590463766135
27	25.312869339158624	29.82762403308335	26.106690508824038	18.75281611893399
28	27.610350578793536	33.57285579819721	23.37773856508272	15.439055057926534
29	31.786109427009734	30.58700303610058	22.60538477325967	15.021502763630016
30	28.402048601199816	30.99464730985447	24.271819466429502	16.331484622516214
31	31.190684573447918	30.14279344843937	21.961599343239783	16.70492263487293
32	26.50277542527819	32.27726415962293	25.240210521846006	15.979749893252873
33	28.331277025895318	34.222067048990446	21.720268271451452	15.726387653662782
34	24.225346131979553	35.99607453662311	23.581088891457636	16.197490439939703
35	25.556559463457095	40.92696609333827	20.27723585099281	13.239238592211825
36	27.880697996456703	37.1390354777907	20.091106607941985	14.889159917810609
37	24.54334641034775	36.49359871101371	21.245862811660324	17.717192066978217
38	24.193970733594558	37.56154178235853	21.96914831127226	16.275339172774647
39	24.335277978952533	37.386500086105414	20.384572740204625	17.893649194737428
40	24.771466788079234	38.944890174310395	20.926683007037052	15.356960030573322
41	21.002880403114894	40.26572367474328	21.99037978386361	16.741016138278223
42	21.698564988358076	41.73824425157879	22.234305813413098	14.328884946650028
43	24.140656146865172	36.89204267997802	22.15339031231496	16.81391086084185
44	24.339524273470804	39.07275082036051	21.177686194116994	15.41003871205169
45	22.430814887508582	37.01825198927103	23.433884014824287	17.117049108396102
46	23.424919615285717	39.84156603341834	20.23005480078981	16.503459550506136
47	23.787034175593718	39.75262975378569	19.80330220170371	16.657033868916887
48	23.639829298960365	38.8458099688841	20.771693257120212	16.74266747503533
49	24.52919209528685	39.274213904727304	18.98895727519999	17.207636724785857
50	21.253647684943818	42.738954326384345	19.91229042767263	16.0951075609992
51	20.812740770796818	41.63822042514844	21.485306641440534	16.063732162614208
52	21.265914757996597	41.44949622433646	19.653030556807163	17.631558460859782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	4.5
2	9.0
3	24.5
4	40.0
5	86.5
6	133.0
7	1749.5
8	3366.0
9	3171.5
10	2977.0
11	3020.0
12	3063.0
13	2482.5
14	1828.5
15	1755.0
16	1824.0
17	1893.0
18	2282.0
19	2671.0
20	3098.0
21	3525.0
22	4141.5
23	4758.0
24	6075.0
25	7392.0
26	9637.5
27	11883.0
28	13299.5
29	14716.0
30	15714.0
31	16712.0
32	17793.0
33	18874.0
34	18552.5
35	18231.0
36	19009.5
37	19788.0
38	19827.5
39	19215.0
40	18563.0
41	18485.5
42	18408.0
43	21251.5
44	24095.0
45	25530.5
46	26966.0
47	30552.0
48	34138.0
49	34316.0
50	34494.0
51	32132.5
52	29771.0
53	25406.0
54	21041.0
55	18037.5
56	15034.0
57	14051.0
58	13068.0
59	11782.5
60	10497.0
61	8423.0
62	6349.0
63	5833.5
64	5701.0
65	6084.0
66	4284.5
67	2485.0
68	2264.5
69	2044.0
70	1534.5
71	1025.0
72	789.0
73	553.0
74	361.5
75	170.0
76	173.0
77	176.0
78	107.5
79	39.0
80	29.0
81	19.0
82	12.0
83	5.0
84	3.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	423899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.706871246036858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46108953085339	21.949586524939047
2	9.356053259729102	5.371660325962136
3	4.082077693221043	3.515510362668469
4	2.3484676973960346	2.696686392584984
5	1.4078268259844806	2.020715171512661
6	0.958293137836152	1.650575863429384
7	0.7234100840835713	1.4536788099310245
8	0.5241520718227085	1.2037412831326364
9	0.39517920658709504	1.0209922742346063
>10	2.668696626912322	15.707171398811653
>50	0.6043423659497503	12.24833254417484
>100	0.43816888365713774	21.376495583839485
>500	0.019841609825983597	4.165130813429217
>1k	0.012401006141239746	5.619722651349882
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2654	0.6260925361937631	No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG	2538	0.5987275270760252	No Hit
TATGTAGGGGTGGGGGGTGTCTCCTGTATTCTTTTAGTTGATGATGCTGTTA	2438	0.5751370019745269	No Hit
TATGTAGGGATGTGTGTCTGTCTGTATGGTGTATGTCTGTCTGTCTGGTGTG	2001	0.4720464072809797	No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1722	0.4062288422477996	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	1643	0.387592327417616	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	1532	0.3614068445549529	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1214	0.28638897473218855	No Hit
TATGTAGGGATTTATAAGTTCTGAGATTGTGCTGTGAGTTTTTTTTTCACAG	1200	0.28308630121797884	No Hit
TATGTAGGGCTTTGGGGGGCTGCCACTGGCTGTTGCGCCTTGTGCCGGCAGC	1148	0.2708192281651997	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAGTGTATACATGTCAGCATGTGT	1143	0.2696397019101248	No Hit
TATGTAGGGCTAAAGATGAGGCAGAAAATGGTTGAATCCCCTGTTTTTTTAT	1137	0.2682242704040349	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1131	0.266808838897945	No Hit
TATGTAGGGATTTGTGAGATGACAGCCTTTTTGTATCTCAAACTGGATGCTT	1102	0.25996758661851055	No Hit
TATGTAGGGATGCAGATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCATGA	1076	0.253834050092121	No Hit
TATGTAGGGAGTCTTGATAGCCTCTGGAAAGCCCTGGCCTGTCGTGTTCCTT	1000	0.23590525101498233	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	993	0.23425391425787745	No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	976	0.23024352499062276	No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC	899	0.2120788206624691	No Hit
TATGTAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA	881	0.20783252614419945	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	873	0.2059452841360796	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	866	0.20429394737897472	No Hit
TATGTAGGGATCCTCACAGGAGTGAAAGGGAAAGAGCTCTGGTCTCTCTTCT	847	0.19981174760969003	No Hit
TATGTAGGGATTTTGGATAGTGTGTCTTGGTACTAATAACTAAATGGATACA	805	0.18990372706706077	No Hit
TATGTAGGGGTGTGCAGCTGAGGGAGAATTTCTCGCTTTCCTGTTGTGGCCA	794	0.187308769305896	No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC	765	0.1804675170264615	No Hit
TATGTAGGGAGCTCTGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTG	743	0.17527760150413188	No Hit
TATGTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	736	0.173626264747027	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	704	0.16607729671454757	No Hit
TATGTAGGGGATTGTTTTCCCCCTCCCAGGGAAATCTATGTACCCTCCCAGC	700	0.16513367571048765	No Hit
TATGTAGGGATAGGCCCTGTTCATGTTTCTTCCTCTCATAGACATGCCCTTC	648	0.15286660265770854	No Hit
TATGTAGGGATTCCCACCACTCCAAATTCTTCCTTGCCTACTTTCTCTCTTC	571	0.1347018983295549	No Hit
TATGTAGGGGGGCTCGCGGACAGCTCCGCGGCCATCTGATTCTTTCGGTTTT	567	0.13375827732549497	No Hit
TATGTAGGGATTCCAGCCCCAGGGCCTTTGCACTTGCTGTTTTTGTTGGCTG	564	0.13305056157245004	No Hit
TATGTAGGGAGACTGTGGCTTCCTTAAGAACCGAGCTTTAGGACAGCAAGTG	558	0.13163513006636016	No Hit
TATGTAGGGACGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAA	528	0.12455797253591067	No Hit
TATGTAGGGATGTGTAAGGGTGTTTTGCAGGCATGTATACTATGTGGGTGTC	519	0.12243482527677585	No Hit
TATGTAGGGATTTAGGGTGCATCTTCTTTCCTCAATTAAACCCTGTACAAAC	507	0.11960396226459605	No Hit
TATGTAGGGATTTCAGGCTGTCATGATGTTGGCCTTCGCGATGCCTTCGCTA	506	0.11936805701358107	No Hit
TATGTAGGGAGCCCTAGCTGCCCTGGAACTTACTTTATAGACCAGGCTAGCC	454	0.10710098396080198	No Hit
TATGTAGGGGCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGA	445	0.10497783670166715	No Hit
TATGTAGGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA	441	0.10403421569760722	No Hit
TATGTAGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAAA	436	0.1028546894425323	No Hit
TATGTAGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA	435	0.10261878419151732	No Hit
TATGTAGGGATGGTACACTGGGTCTCCAGGCTAGCTCCGACATGGTACACTG	433	0.10214697368948736	No Hit
TATGTAGGGAGGGATTGAACTTAGGGCTTGCAAAGTACAGACAAGCACAGTA	428	0.10096744743441244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.1389481928478246	0.0	0.0	0.0	0.0
7	0.14059952960492947	0.0	0.0	0.0	0.0
8	0.14366629786812424	0.0	0.0	0.0	0.0
9	0.15192298165364862	0.0	0.0	0.0	0.0
10	0.23684887201904228	0.0	0.0	0.0	0.0
11	0.3724943913526571	0.0	0.0	0.0	0.0
12	0.46685649175865007	0.0	0.0	0.0	0.0
13	0.5185197417309312	0.0	0.0	0.0	0.0
14	0.6050969688534297	0.0	0.0	0.0	0.0
15	0.6845970384454788	0.0	0.0	0.0	0.0
16	0.7445169722032843	0.0	0.0	0.0	0.0
17	0.7829695281187264	0.0	0.0	0.0	0.0
18	0.827083810058528	0.0	0.0	0.0	0.0
19	0.8752084812655845	0.0	0.0	0.0	0.0
20	0.9238049629746709	0.0	0.0	0.0	0.0
21	0.9660320029063527	0.0	0.0	0.0	0.0
22	1.0176952528786338	0.0	0.0	0.0	0.0
23	1.0771433761344094	0.0	0.0	0.0	0.0
24	1.135647878386125	0.0	0.0	0.0	0.0
25	1.1858956968523162	0.0	0.0	0.0	0.0
26	1.2517132618854963	0.0	0.0	0.0	0.0
27	1.321777121436946	0.0	0.0	0.0	0.0
28	1.4378425049363173	0.0	0.0	0.0	0.0
29	1.480777260621044	0.0	0.0	0.0	0.0
30	1.5425844363869696	0.0	0.0	0.0	0.0
31	1.5829242343105314	0.0	0.0	0.0	0.0
32	1.6239717479871385	0.0	0.0	0.0	0.0
33	1.668793745679985	0.0	0.0	0.0	0.0
34	1.7275341531827157	0.0	0.0	0.0	0.0
35	1.8525639362206563	0.0	0.0	0.0	0.0
36	1.9011604179297428	0.0	0.0	0.0	0.0
37	1.9459824156225893	0.0	0.0	0.0	0.0
38	2.003071486368215	0.0	0.0	0.0	0.0
39	2.058037409854706	0.0	0.0	0.0	0.0
40	2.1217318276287513	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAT	25	3.4134813E-5	46.0	20
TGATCCG	20	6.3069246E-4	46.0	35
TCGCCTA	20	6.3069246E-4	46.0	19
ATCCTTG	20	6.3069246E-4	46.0	37
CTTCGTC	20	6.3069246E-4	46.0	23
TCGCCAC	20	6.3069246E-4	46.0	30
ATCCTGC	25	3.4134813E-5	46.0	26
GTCATAC	20	6.3069246E-4	46.0	44
TTTAGAA	20	6.3069246E-4	46.0	25
AGACTTA	25	3.4134813E-5	46.0	46
GTGCAAG	40	5.5970304E-9	46.0	20
GAAATCA	20	6.3069246E-4	46.0	29
TGTCAAT	20	6.3069246E-4	46.0	23
TTAGGAC	40	5.5970304E-9	46.0	38
TCGTTCT	25	3.4134813E-5	46.0	12
GTCGCGT	25	3.4134813E-5	46.0	10
AGATCTT	25	3.4134813E-5	46.0	44
TGCCCGT	30	1.8586034E-6	46.0	46
CGAACCT	45	3.092282E-10	46.0	38
ACCGGAC	20	6.3069246E-4	46.0	40
>>END_MODULE