FastQCFastQC Report
Fri 17 Jun 2016
SRR1527153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43144
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA25015.7968663081772664No Hit
CATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT20424.732987205636937No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC13683.1707769330613758No Hit
CATCCCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG7991.8519376970146484No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGA7661.7754496569627294No Hit
CATCCCGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA5101.1820878917114779No Hit
CATCCCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG5081.1774522529204525No Hit
CATCCCGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC3970.9201743000185426No Hit
CATCCCGGGGAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTATG3900.9039495642499537No Hit
CATCCCGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCC2600.6026330428333024No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG2560.5933617652512516Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG2460.5701835712961246No Hit
CATCCCGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT2400.5562766549230485No Hit
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATT2000.4635638791025403No Hit
CATCCCGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCT1980.4589282403115149No Hit
CATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTA1850.42879658816984983No Hit
CATCCCGGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGC1720.3986649360281847No Hit
CATCCCGGGGGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG1690.3917114778416466No Hit
CATCCCGGGAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTT1630.3778045614685704No Hit
CATCCCAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT1610.373168922677545No Hit
CATCCCGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAA1590.36853328388651957No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTC1460.33840163174485444No Hit
CATCCCGGGGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAA1400.3244947153717782No Hit
CATCCCGGGGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCT1360.3152234377897274No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1230.2850917856480623No Hit
CATCCCGGGGGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGA1210.2804561468570369No Hit
CATCCCGGGGCTCATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGA1130.2619135916929353No Hit
CATCCCAGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1130.2619135916929353No Hit
CATCCCGGGGAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAA1070.24800667531985907No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1030.23873539773780825No Hit
CATCCCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGT1010.23409975894678287No Hit
CATCCCAGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCG960.22251066196921934No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATA950.22019284257370664No Hit
CATCCCGGGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCTCAAGTA930.21555720378268126No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC910.21092156499165582No Hit
CATCCCGGGGGTCACGGTGTAGTGGGACCTCATTTCCCTCACCTTTGGCA910.21092156499165582No Hit
CATCCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCAT870.20165028740960506No Hit
CATCCCCTTCCCAGACTCGGCCCCAGCACAGGTTAGAGTGCCTTTCCTTT850.19701464861857965No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT840.19469682922306694No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA840.19469682922306694Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGC810.18774337103652883No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCAC780.18078991284999074No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA740.17151863526793992Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC710.16456517708140184No Hit
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCT690.15992953829037643Illumina Single End Adapter 2 (96% over 29bp)
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTGATCCCAGC650.1506582607083256No Hit
CATCCCGGGGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGAG620.14370480252178752No Hit
CATCCCGGGGTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCC610.1413869831262748No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT580.1344335249397367No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATA580.1344335249397367No Hit
CATCCCGGGAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGC570.132115705544224No Hit
CATCCCGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCC570.132115705544224No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA570.132115705544224Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCT560.1297978861487113No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA550.1274800667531986Illumina Single End Adapter 2 (97% over 34bp)
CATCCCTGGGAAGGATACCTTGGCAGATTGAGGAAGGCTATGGAAAGTTC550.1274800667531986No Hit
CATCCCGGGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTT540.12516224735768589No Hit
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT540.12516224735768589No Hit
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAG530.12284442796217318No Hit
CATCCCGGGATGAGCACAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA530.12284442796217318Illumina Single End Adapter 2 (96% over 26bp)
CATCCCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC480.11125533098460967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA302.4712626E-644.0000049
GGATGGT551.8189894E-1244.0000048
GGATGAA600.044.0000048
CGGGGCT600.044.0000046
GATGTGG302.4712626E-644.0000049
GCTGCAA302.4712626E-644.00000442
CGGGAGC551.8189894E-1244.0000046
CGGGACA1100.044.0000046
CGGGAAC302.4712626E-644.0000046
TGTTGTG407.996277E-944.028
CGTTTGC407.996277E-944.012
CGTTTGA454.5656634E-1044.024
GGATGTA254.3717075E-544.08
GGATGGG700.044.08
GGATGAC254.3717075E-544.08
CATGACC207.772342E-444.041
CCGGACT254.3717075E-544.05
GCCTCAG254.3717075E-544.026
CGTTATT207.772342E-444.032
AGCTCCA254.3717075E-544.035
Produced by FastQC (version 0.11.5)