FastQCFastQC Report
Fri 17 Jun 2016
SRR1527152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45668
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA22845.001313830253131No Hit
CATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT18794.114478409389507No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC12232.6780239992992905No Hit
CATCCCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA8401.8393623543838136No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG5061.1079968468073924No Hit
CATCCCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT4611.0094595778225453No Hit
CATCCCGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA4410.9656652360515022No Hit
CATCCCGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA4060.8890251379521766No Hit
CATCCCGGGGAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTATGTG3320.7269860733993168No Hit
CATCCCGGGGACATTAGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA2760.6043619164403959No Hit
CATCCCGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCT2490.5452395550494876No Hit
CATCCCGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG2410.5277218183410703No Hit
CATCCCGGGGACTTCAGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT2190.4795480423929228No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT1940.42480511517911884No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA1930.42261539809056675Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATG1770.38757992467373215No Hit
CATCCCGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGC1700.37225190505386707No Hit
CATCCCGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGC1640.3591136025225541No Hit
CATCCCGGGGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACT1580.34597529999124116No Hit
CATCCCGGGGGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGG1570.34378558290268896No Hit
CATCCCGGGAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTG1490.3262678461942717No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCTC1480.32407812910571954No Hit
CATCCCGGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTG1380.30218095822019797No Hit
CATCCCGGGACTTCTAATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC1340.2934220898659893No Hit
CATCCCGGGGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG1290.2824735044232285No Hit
CATCCCAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT1290.2824735044232285No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCA1230.26933520189191557No Hit
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTA1180.2583866164491548No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAG1120.2452483139178418No Hit
CATCCCGGGACATTTACTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA1120.2452483139178418No Hit
CATCCCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC1110.24305859682928968No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAA1100.24086887974073748No Hit
CATCCCGGGGGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA1090.23867916265218536No Hit
CATCCCAGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1030.22554086012087238No Hit
CATCCCGGGGGTCACGGTGTAGTGGGACCTCATTTCCCTCACCTTTGGCATG990.21678199176666377No Hit
CATCCCGGGGCTCATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAA980.2145922746781116No Hit
CATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGC960.21021284050100728No Hit
CATCCCGGGGAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAA950.2080231234124551No Hit
CATCCCAGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCG850.18612595252693354No Hit
CATCCCCTTCCCAGACTCGGCCCCAGCACAGGTTAGAGTGCCTTTCCTTTGT840.18393623543838136No Hit
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCG840.18393623543838136No Hit
CATCCCGGGGACATTGGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA840.18393623543838136No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC810.1773670841727249No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG780.1707979329070684No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA760.1664184987299641No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG720.15765963037575545No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA720.15765963037575545Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTGATCCCAGCCG720.15765963037575545No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC710.1554699132872033No Hit
CATCCCGGGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCTCAAGTAGC680.14890076202154684No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA680.14890076202154684Illumina Single End Adapter 2 (97% over 34bp)
CATCCCTGGGAAGGATACCTTGGCAGATTGAGGAAGGCTATGGAAAGTTCTT670.14671104493299464No Hit
CATCCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCT650.14233161075589035No Hit
CATCCCGGGGACTTCGGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT610.1335727424016817No Hit
CATCCCGGGAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCT600.13138302531312954No Hit
CATCCCGGGGTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA600.13138302531312954No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT570.12481387404747306No Hit
CATCCCGGGGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGAGAT560.1226241569589209No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA560.1226241569589209Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA540.1182447227818166No Hit
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG540.1182447227818166No Hit
CATCCCGGGACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGG520.11386528860471228No Hit
CATCCCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG500.10948585442760794No Hit
CATCCCGGGGACACAATTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA500.10948585442760794No Hit
CATCCCGGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG490.1072961373390558No Hit
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC480.10510642025050364Illumina Single End Adapter 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGA206.2476634E-446.00000424
GATGGAT206.2476634E-446.0000049
GATGGAG206.2476634E-446.0000049
CGCTGAA206.2476634E-446.00000442
CGTCAAC206.2476634E-446.0000046
AAGTAGC206.2476634E-446.00000446
CATGACC206.2476634E-446.00000441
CGTTATT206.2476634E-446.00000432
TGACCCG206.2476634E-446.00000443
GCGATGC206.2476634E-446.00000420
CACGTTT206.2476634E-446.00000422
CCACGTT206.2476634E-446.00000421
ATGACCC206.2476634E-446.00000442
TGCCGAC206.2476634E-446.00000411
AATCACT206.2476634E-446.00000422
GTCAACA206.2476634E-446.0000047
TACGGAT206.2476634E-446.00000413
TAGCTTC405.4096745E-946.00000415
GATGCGG206.2476634E-446.00000422
TTATTCC206.2476634E-446.00000434
Produced by FastQC (version 0.11.5)