FastQCFastQC Report
Fri 17 Jun 2016
SRR1527151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44201
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA23295.269111558562024No Hit
CATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT18464.176376100088233No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC11872.6854596049863124No Hit
CATCCCGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA7421.6786950521481416No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGAAG4981.126671342277324No Hit
CATCCCGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT4511.0203389063595847No Hit
CATCCCGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA4410.9977149838238953No Hit
CATCCCGGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA3550.8031492500169679No Hit
CATCCCGGGGAGTACGGATTCCACGTTTGAGTCCCAACATCTCCAGTATGTG3020.683242460577815No Hit
CATCCCGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCT2210.49998868803873214No Hit
CATCCCGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG1940.4389040971923712No Hit
CATCCCGGGGGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGG1760.39818103662813065No Hit
CATCCCGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTGC1730.3913938598674238No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT1680.3800818985995792No Hit
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCATCCCGGGATG1660.3755571140924413No Hit
CATCCCGGGAACGATGGCCTGAATCACTTGGACAGCCTCAAGGGCACCTTTG1570.355195583810321No Hit
CATCCCGGGGGGTGCCCTGTCTGCTCTGAGCGACCTGCATGCCCACAAGCTG1530.34614601479604534No Hit
CATCCCAGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT1510.3416212302889075No Hit
CATCCCGGGATGAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA1500.33935883803533856Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGACATTTGCTTGTGACATAGTTGTGTTGACACACAACCCCAGA1450.32804687676749394No Hit
CATCCCGGGGCCCAGGTCAAGGGTCACGGCAAGAAGGTCGCCGATGCTCTGG1430.3235220922603561No Hit
CATCCCGGGAAGGGCACCTTTGCCAGCCTCAGTGAGCTCCACTGTGACAAGC1420.32125970000678716No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCA1340.3031605619782358No Hit
CATCCCGGGACTTCTGATTCTGACAGACTCAGGAGGAAACCATGGTGCTCTC1320.29863577747109793No Hit
CATCCCGGGGCTCATCCCGGGGACTTCTGATTCTGACAGACTCAGGAAGAAA1300.2941109929639601No Hit
CATCCCGGGGGGTAATGCCAAAGTGAAGGCCCATGGCAAGAAAGTGATAACT1230.27827424718897764No Hit
CATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTA1160.2624375014139952No Hit
CATCCCGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC1100.24886314789258163No Hit
CATCCCGGGGGACAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACA1040.23528879437116806No Hit
CATCCCGGGGGTCACGGTGTAGTGGGACCTCATTTCCCTCACCTTTGGCATG1000.2262392253568924No Hit
CATCCCAGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA990.22397683310332347No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAG950.21492726408904778No Hit
CATCCCGGGGAGACTCAGGAAGAAACCATGGTGCTCTCTGGGGAAGACAAAA940.21266487183547886No Hit
CATCCCGGGGACTTCTGATTGTGACAGACTCAGGAAGAAACCATGGTGCTCT920.20814008732834097No Hit
CATCCCAGGGGGAATTCGGGCTGGAGATCATCCTACAAGGGCGCGACCCGCG870.19682812606049635No Hit
CATCCCGGGGACATTTGCTTGTGACATAGTTGTGTTGACGCACAACCCCAGA850.19230334155335851No Hit
CATCCCCTTCCCAGACTCGGCCCCAGCACAGGTTAGAGTGCCTTTCCTTTGT840.1900409492997896No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAA840.1900409492997896No Hit
CATCCCGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCCG790.17872898803194498No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG770.17420420352480712No Hit
CATCCCGAAGTTGCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGC760.1719418112712382No Hit
CATCCCGGGATGAGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA740.16741702676410036Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC730.16515463451053145No Hit
CATCCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCT720.16289224225696253No Hit
CATCCCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA650.14705549648198005No Hit
CATCCCGGGATCGGTGCCACCCACTCCTCCCGATTCATCCCCCTCAAGTAGC640.14479310422841113No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC630.14253071197484218No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT620.14026831972127327No Hit
CATCCCGGGGACTTCTGATTGTGACAGACTCAGGAAGAAGCCATGGTGCTCT610.13800592746770435No Hit
CATCCCGGGACCTGACTGATGCTGAGAAGGCTGCTGTCTCTGGCCTGTGGGG590.1334811429605665No Hit
CATCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG590.1334811429605665No Hit
CATCCCGGGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGCGGTACTTTG580.1312187507069976No Hit
CATCCCGGGACTTCTGATTCGGACAGACTCAGGAAGAAAACATGGTGCTCTC580.1312187507069976No Hit
CATCCCGGGGTCACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA580.1312187507069976No Hit
CATCCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTGATCCCAGCCG580.1312187507069976No Hit
CATCCCTGGGAAGGATACCTTGGCAGATTGAGGAAGGCTATGGAAAGTTCTT570.12895635845342865No Hit
CATCCCGGGATGAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA560.12669396619985973Illumina Single End Adapter 2 (100% over 34bp)
CATCCCGGGACTTCTGATTCGGACAGACTCAGGAAGAAAGCATGGTGCTCTC550.12443157394629081No Hit
CATCCCGGGATGAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC540.12216918169272188Illumina Single End Adapter 2 (96% over 31bp)
CATCCCGGGAGGCCCATGGCAAGAAAGTGATAACTGCCTTTAACGATGGCCT520.11764439718558403No Hit
CATCCCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG520.11764439718558403No Hit
CATCCCGGGATGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA510.11538200493201511Illumina Single End Adapter 2 (97% over 34bp)
CATCCCGGGGGACCCAGCGGTACTTTGATAGCTTTGGAGACCTATCCTCTGC490.11085722042487725No Hit
CATCCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA450.10180765141060157Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC359.9005774E-846.00000419
CAGGGGA359.9005774E-846.0000046
CGGGATC700.046.0000046
GACAAGC359.9005774E-846.00000446
CCTTTGC359.9005774E-846.00000417
CCTCAGT359.9005774E-846.00000427
GGGATGT1400.046.0000047
CGCCGGA359.9005774E-846.00000446
CGTTTGA301.8208266E-646.024
TTATGAC206.245493E-446.019
GGATGTG452.9649527E-1046.08
GGATGTA405.4042175E-946.08
TTTGACA206.245493E-446.020
ACTGTGA405.4042175E-946.041
GGATGCT405.4042175E-946.08
GGATGCA650.046.08
GTTGACG253.3637607E-546.034
GTTGACA206.245493E-446.034
TTGCAAC550.046.040
CCGGACT206.245493E-446.05
Produced by FastQC (version 0.11.5)