FastQCFastQC Report
Fri 17 Jun 2016
SRR1527150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511891
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC38860.7591459900642911No Hit
ACCTGAGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAA37460.731796417596715No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23850.4659195023940643No Hit
ACCTGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT13780.2691979347165705No Hit
ACCTGAGGGGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCT12180.2379412804679121No Hit
ACCTGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA11360.22192224516547468No Hit
ACCTGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.21528020613763477No Hit
ACCTGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA10430.20375431488344198No Hit
ACCTGAGGGGGGTTCTGATCAGGACAATTGAGACCCGGGATGGGGAGAAG10240.20004258719141377No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG9750.19047023682776215No Hit
ACCTGAGGGAGTTAATTCAAGTTTAGACCACTTCTAGTGTTTTTAAAAAG8790.1717162442785671No Hit
ACCTGAGGGGATTCGACATTTGTATTCATAGAAGTTCACATGGGTTTTGT8610.16819987067559306No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGGA8430.16468349707261898No Hit
ACCTGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA8250.1611671234696449No Hit
ACCTGAGGGATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATC8250.1611671234696449No Hit
ACCTGAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG8190.1599949989353202No Hit
ACCTGAGGGGCCAATAACCAACCTTATTCAGTAAACCACTTGAATCTTGT8190.1599949989353202No Hit
ACCTGAGGGGCTAGTTATTTTGTCAAATGCATGTAATGAACAGACCCGAA8120.1586275203119414No Hit
ACCTGAGGGATCTACGCATGTCCCATGACAATTGTTTGTTATTTGTTAAG7770.15179012719504736No Hit
ACCTGAGGGGATCAAGGTGAATCCTCATCCCCGTTGTTGCGCGGTGCTCA7770.15179012719504736No Hit
ACCTGAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGC7280.14221777683139575No Hit
ACCTGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA7250.1416317145642334No Hit
ACCTGAGGGGTGGCGCCTCGCTTTCAGCCTGGGCTGGCATTCTCCCTAGC7140.13948281958463812No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT6860.13401290509112293No Hit
ACCTGAGGGGGCTAAGTAATATTGTGTGTGTGTGTGTGTGTGTGTGTGTG6790.1326454264677441No Hit
ACCTGAGGGGAACAGGGGACACCTTCGGTGGCAGTGAGGTAGACCCCAGG6770.13225471828963586No Hit
ACCTGAGGGACAATATTTCCTGTGCTCTCCAGAGACGATGGACCGATTTC6740.13166865602247355No Hit
ACCTGAGGGGAGTCTGAGACTGTGAATGGGGTAAGTGCTTTCTTTTAGTG6650.1299104692209865No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT6650.1299104692209865No Hit
ACCTGAGGGTGCAGTGCTACTTGAGGGGTGGTATCAACGCAGGGTGCAGT6620.12932440695382416No Hit
ACCTGAGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACAC6520.12737086606328302No Hit
ACCTGAGGGACAGAAGAATTTTATGGATCCTTAACTCACAATAAGTTTGA6500.12698015788517478No Hit
ACCTGAGGGGGACATGCGCTCACTCGCGCACACACATACACACAACATAC6320.12346378428220071No Hit
ACCTGAGGGGACAGTGAACGGGTATGTGTTTGCCATTTGCTGGAGTTGCC6240.12190095156976778No Hit
ACCTGAGGGAGCAGCAGCTTTGATAGTTCTCACGGAGTCTGGCAGGAGTG6180.12072882703544309No Hit
ACCTGAGGGAAGGTTTGCTGTCCAAGGTGTAAATACTCAACGGGAATAAA6100.11916599432301017No Hit
ACCTGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG6080.11877528614490193No Hit
ACCTGAGGGGCCCGCAACCTGACCCAGCAGCTCCAGACTACATGCCAGAC6080.11877528614490193No Hit
ACCTGAGGGAGTTCTGCACAAACTATTTCACAGGATAGAAGTAGAAGGTA6060.11838457796679372No Hit
ACCTGAGGGCGAGTGTTAGCTGTAGGCTCCTGTACTGTTTGTGTGCACTT6030.11779851569963136No Hit
ACCTGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA6010.11740780752152315No Hit
ACCTGAGGGGTTTAATGCTGGCCTAACTATTACCAAGGCCATGCTGGAGA5980.11682174525436079No Hit
ACCTGAGGGATGACAGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCA5930.11584497480909021No Hit
ACCTGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT5910.11545426663098197No Hit
ACCTGAGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGA5800.11330537165138672No Hit
ACCTGAGGGATCAAAAACTGGTGTTCTTTGCTGAAGATGTGGGTTCGAAC5770.11271930938422438No Hit
ACCTGAGGGACTGCACAATCAAAATGGCATCTATAGTAATGACCTCCAGA5750.11232860120611615No Hit
ACCTGAGGGGCTTGGCACAGGGCTTGACACATTTAGGGAAGGGCACACTT5750.11232860120611615No Hit
ACCTGAGGGTGCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGG5680.11096112258273734No Hit
ACCTGAGGGATCTGCCTCCCCTTAGCTCTTCAGAAGCGCCCCTCCCCTGT5670.11076576849368322No Hit
ACCTGAGGGAATTCTTACATTTTGAGATAACTGTAAAAAGAGTGAAATTA5550.10842151942503384No Hit
ACCTGAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT5540.10822616533597972No Hit
ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA5450.10646797853449269No Hit
ACCTGAGGGGTGCAGTGCTACCTGAGGGAGCGTCATCCTCGCCAGAGTGT5390.105295854000168No Hit
ACCTGAGGGATACTAAAGAAAGCTTAGTTACTGTATTGTTCAGTGAAAGT5390.105295854000168No Hit
ACCTGAGGGGAGGGGTTGGTGCCTCTGAAGCCACCAACCCCTATGCTGGG5330.1041237294658433No Hit
ACCTGAGGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAAC5330.1041237294658433No Hit
ACCTGAGGGTGCTGTGAACTCATCTTTCTGGTATGCTCCTTTGTGTCTCA5290.10334231310962685No Hit
ACCTGAGGGGCTGCCATTGCCACCATCAAGACCAAGCGTACCATCCAGTT5230.10217018857530216No Hit
ACCTGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT5200.10158412630813982No Hit
ACCTGAGGGTGATTGGCTTAGGGTACACTCAGACCCTAAAACCAGGTATC5190.1013887722190857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT207.852385E-444.027
ATGGGTT1150.044.040
AAGTATG408.296411E-944.018
TTCTACG254.439368E-544.036
AACCGTT207.852385E-444.019
CGGGTAA207.852385E-444.031
ACTATAC408.296411E-944.023
TGATACC254.439368E-544.033
CGGCGCA502.7284841E-1144.027
TCTGACG207.852385E-444.044
GTACCGG207.852385E-444.041
ACACGAT254.439368E-544.023
CGTATTA207.852385E-444.027
TCCGCGT207.852385E-444.012
GTCGATT207.852385E-444.028
GTACATC302.5249556E-644.032
ATAGGTA207.852385E-444.042
GTCGAGG207.852385E-444.014
GTCGACC254.439368E-544.010
CTCAACG700.044.036
Produced by FastQC (version 0.11.5)