FastQCFastQC Report
Fri 17 Jun 2016
SRR1527149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547143
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA42280.7727413126001794No Hit
ACCTGAGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC34280.6265272515594643No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC21300.389294937520904No Hit
ACCTGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC13150.24033936283567547No Hit
ACCTGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG11870.2169451130691611No Hit
ACCTGAGGGGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTA10520.1922714902685404No Hit
ACCTGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.19007827935292967No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9870.1803915978089823No Hit
ACCTGAGGGGGGTTCTGATCAGGACAATTGAGACCCGGGATGGGGAGAAGGT9780.17874668962227425No Hit
ACCTGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG9650.17637071113036262No Hit
ACCTGAGGGATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTG8770.16028716441588398No Hit
ACCTGAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC8250.15078325044823748No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGGAAG8080.1476762016511223No Hit
ACCTGAGGGAGTTAATTCAAGTTTAGACCACTTCTAGTGTTTTTAAAAAGGG7990.14603129346441424No Hit
ACCTGAGGGGATTCGACATTTGTATTCATAGAAGTTCACATGGGTTTTGTAA7950.14530022315921068No Hit
ACCTGAGGGATCTACGCATGTCCCATGACAATTGTTTGTTATTTGTTAAGGC7860.1436553149725026No Hit
ACCTGAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCC7750.14164487163319278No Hit
ACCTGAGGGGATCAAGGTGAATCCTCATCCCCGTTGTTGCGCGGTGCTCAAA7400.1352480064626615No Hit
ACCTGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA7340.13415140100485615No Hit
ACCTGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG7040.12866837371582932No Hit
ACCTGAGGGGCCAATAACCAACCTTATTCAGTAAACCACTTGAATCTTGTCC6960.12720623310542217No Hit
ACCTGAGGGACAGAAGAATTTTATGGATCCTTAACTCACAATAAGTTTGACT6930.12665793037651948No Hit
ACCTGAGGGGGCTAAGTAATATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG6880.12574409249501503No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6820.12464748703720965No Hit
ACCTGAGGGGAGTCTGAGACTGTGAATGGGGTAAGTGCTTTCTTTTAGTGCA6730.12300257885050161No Hit
ACCTGAGGGGCTAGTTATTTTGTCAAATGCATGTAATGAACAGACCCGAAGG6700.12245427612159893No Hit
ACCTGAGGGTGCAGTGCTACTTGAGGGGTGGTATCAACGCAGGGTGCAGTCC6630.12117490308749265No Hit
ACCTGAGGGGCCCGCAACCTGACCCAGCAGCTCCAGACTACATGCCAGACTG6600.12062660035858999No Hit
ACCTGAGGGACAATATTTCCTGTGCTCTCCAGAGACGATGGACCGATTTCCT6520.11916445974818282No Hit
ACCTGAGGGAGCAGCAGCTTTGATAGTTCTCACGGAGTCTGGCAGGAGTGCT6370.11642294610366942No Hit
ACCTGAGGGGAACAGGGGACACCTTCGGTGGCAGTGAGGTAGACCCCAGGCC6300.11514357306956317No Hit
ACCTGAGGGATCAAAAACTGGTGTTCTTTGCTGAAGATGTGGGTTCGAACAA6260.11441250276435959No Hit
ACCTGAGGGAGTTCTGCACAAACTATTTCACAGGATAGAAGTAGAAGGTACT6000.10966054578053636No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA5980.10929501062793456No Hit
ACCTGAGGGATGACAGAGTCCCTTCTTCTGAGAAGGGAAAACATTGATCACC5960.10892947547533277No Hit
ACCTGAGGGGTGGCGCCTCGCTTTCAGCCTGGGCTGGCATTCTCCCTAGCAG5890.10765010244122651No Hit
ACCTGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA5840.10673626455972206No Hit
ACCTGAGGGGGACATGCGCTCACTCGCGCACACACATACACACAACATACAC5840.10673626455972206No Hit
ACCTGAGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA5700.10417751849150954No Hit
ACCTGAGGGGTGCAGTGCTACCTGAGGGAGCGTCATCCTCGCCAGAGTGTTC5660.10344644818630595No Hit
ACCTGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG5640.10308091303370416No Hit
ACCTGAGGGAGAGTGAGTATGGCTGCGGGGTCGCGGCTCTGAAATCCGGCGC5590.1021670751521997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT501.6370905E-1146.038
TCACGCC206.308541E-446.021
ACAACGA206.308541E-446.041
TTTAGGA206.308541E-446.025
TGTTTAG206.308541E-446.023
CGGAATA206.308541E-446.019
CGGGTAT900.046.019
CGGGTAA253.4147924E-546.031
GTCGCTA453.092282E-1046.015
CTGTCGA253.4147924E-546.044
TTGGCGC301.859602E-646.021
TCCATCG453.092282E-1046.046
ACAGTCG206.308541E-446.046
CGAACTT405.6024874E-946.024
ACACGTA206.308541E-446.039
ACACGGT501.6370905E-1146.031
ATTAGTC206.308541E-446.011
GCGACGT253.4147924E-546.038
GTCGATT206.308541E-446.028
CATATAT253.4147924E-546.038
Produced by FastQC (version 0.11.5)