FastQCFastQC Report
Fri 17 Jun 2016
SRR1527148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532376
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA39800.7475919275098802No Hit
ACCTGAGGGATACAAAAACTACTCCAGTGTCTTGTACTGACTGCTGCCAATC31580.5931897756472869No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC20440.38393917081160683No Hit
ACCTGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC12000.22540460125926037No Hit
ACCTGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG10700.20098576945617383No Hit
ACCTGAGGGGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTA10390.19516281725697626No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9940.186710144709754No Hit
ACCTGAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9930.18652230754203797No Hit
ACCTGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG9250.17374938013734653No Hit
ACCTGAGGGGGGTTCTGATCAGGACAATTGAGACCCGGGATGGGGAGAAGGT9140.17168317129247No Hit
ACCTGAGGGAGTTAATTCAAGTTTAGACCACTTCTAGTGTTTTTAAAAAGGG8810.16548454475784033No Hit
ACCTGAGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC8350.156844035042902No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACCTGAGGGAAG8150.153087291688581No Hit
ACCTGAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA7590.14256841029648218No Hit
ACCTGAGGGATCTACAAATGGACTGTTTACCTTGACTACCAATGGGGATCTG7390.13881166694216118No Hit
ACCTGAGGGGCTAGTTATTTTGTCAAATGCATGTAATGAACAGACCCGAAGG7350.13806031827129697No Hit
ACCTGAGGGGATTCGACATTTGTATTCATAGAAGTTCACATGGGTTTTGTAA7210.13543059792327228No Hit
ACCTGAGGGATCTACGCATGTCCCATGACAATTGTTTGTTATTTGTTAAGGC7190.1350549235878402No Hit
ACCTGAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG7090.13317655191067967No Hit
ACCTGAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCC6900.1296076457240747No Hit
ACCTGAGGGGATCAAGGTGAATCCTCATCCCCGTTGTTGCGCGGTGCTCAAA6650.12491171653117344No Hit
ACCTGAGGGACAGAAGAATTTTATGGATCCTTAACTCACAATAAGTTTGACT6620.12434820502802531No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA6490.12190632184771666No Hit
ACCTGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC6460.12134281034456851No Hit
ACCTGAGGGGCCAATAACCAACCTTATTCAGTAAACCACTTGAATCTTGTCC6420.1205914616737043No Hit
ACCTGAGGGGGCTAAGTAATATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG6300.1183374156611117No Hit
ACCTGAGGGGAGTCTGAGACTGTGAATGGGGTAAGTGCTTTCTTTTAGTGCA6270.11777390415796354No Hit
ACCTGAGGGTGCAGTGCTACTTGAGGGGTGGTATCAACGCAGGGTGCAGTCC6230.11702255548709933No Hit
ACCTGAGGGGCCCGCAACCTGACCCAGCAGCTCCAGACTACATGCCAGACTG6130.11514418380993885No Hit
ACCTGAGGGGAACAGGGGACACCTTCGGTGGCAGTGAGGTAGACCCCAGGCC6000.11270230062963019No Hit
ACCTGAGGGGCTTGGCACAGGGCTTGACACATTTAGGGAAGGGCACACTTAC5940.11157527762333388No Hit
ACCTGAGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACG5700.10706718559814866No Hit
ACCTGAGGGAGTTCTGCACAAACTATTTCACAGGATAGAAGTAGAAGGTACT5680.10669151126271657No Hit
ACCTGAGGGATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTA5680.10669151126271657No Hit
ACCTGAGGGGTGGCGCCTCGCTTTCAGCCTGGGCTGGCATTCTCCCTAGCAG5670.10650367409500053No Hit
ACCTGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA5570.10462530241784002No Hit
ACCTGAGGGACAATATTTCCTGTGCTCTCCAGAGACGATGGACCGATTTCCT5570.10462530241784002No Hit
ACCTGAGGGTGCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGGGGCA5460.10255909357296347No Hit
ACCTGAGGGAAGGTTTGCTGTCCAAGGTGTAAATACTCAACGGGAATAAAAC5400.10143207056666717No Hit
ACCTGAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC5400.10143207056666717No Hit
ACCTGAGGGAGCAGCAGCTTTGATAGTTCTCACGGAGTCTGGCAGGAGTGCT5380.10105639623123507No Hit
ACCTGAGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC5360.10068072189580296No Hit
ACCTGAGGGGGACATGCGCTCACTCGCGCACACACATACACACAACATACAC5340.10030504756037087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATCG351.0181793E-746.00000420
AACCATA551.8189894E-1246.00000444
CGATTAA351.0181793E-746.00000430
TCGATTA351.0181793E-746.00000429
ACGGGTA551.8189894E-1246.00000418
ATCACAT351.0181793E-746.00000437
TACGCAT1400.046.00000413
CGGTGTG700.046.00000433
TCGTAGA700.046.00000415
TTACGTT351.0181793E-746.00000412
CAATCGC351.0181793E-746.00000432
ATCGCGC351.0181793E-746.00000434
ATCGCAA351.0181793E-746.00000428
TCGCGCG351.0181793E-746.00000435
AATCGCG351.0181793E-746.00000433
TACCGCC551.8189894E-1246.00000444
CCATTGA351.0181793E-746.00000411
GCGAGAC206.308387E-446.021
TTTAGTA301.8595074E-646.030
GCCCCGT206.308387E-446.021
Produced by FastQC (version 0.11.5)