FastQCFastQC Report
Fri 17 Jun 2016
SRR1527147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412839
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC155781.1026026320054867No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG124920.8841771780082515No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAG62330.44116845585378095No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT42330.2996095096468883No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTG41300.2923192239172333No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC37480.2652814651917168No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG35870.2538859700220619No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC34450.2438352848413726No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC33400.23640344016551074No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC30320.21460336244964925No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATT28790.20377410306482197No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT27070.19160003369102918No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG25910.18338961481102942No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC24640.17440062172689175No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC23360.1653408491696506No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT22730.1608817423641335No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT20270.1434699919806857No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA20190.14290375619585813No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAA18120.12825240526344472No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGT17420.1232978421462035No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG17220.12188225268413458No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC17150.12138679637241044No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC16090.11388417222344513No Hit
TGCGGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG15640.11069909593379006No Hit
TGCGGAGGGAACCTTTGATGACTCTGTATTTCGTTTTTGAGACTGTATTC15310.10836337332137633No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCT14900.10546141492413501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.857094E-444.00000410
CGAACGC207.857094E-444.00000427
GTCGATA207.857094E-444.00000444
TTTACGG207.857094E-444.00000413
CGAAAAC207.857094E-444.00000413
CAACGAA207.857094E-444.00000419
CGCATTA207.857094E-444.00000419
CCAATCG302.5281297E-644.00000428
CGTCTTA408.314601E-944.00000416
TTCGTAC207.857094E-444.00000419
CGACACG207.857094E-444.00000438
CGTTATG207.857094E-444.00000424
GTAATCG207.857094E-444.00000444
TTATACG302.5281297E-644.00000441
CGTTAAC207.857094E-444.00000425
AAACGTA302.5281297E-644.00000435
GTTACGT650.044.00000444
ACGATAT207.857094E-444.00000426
TACGCGA207.857094E-444.00000414
CGCTATC207.857094E-444.00000433
Produced by FastQC (version 0.11.5)