##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412839
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.111187474298205	31.0	31.0	34.0	31.0	34.0
2	32.62249414122911	34.0	31.0	34.0	31.0	34.0
3	32.8491859298901	34.0	31.0	34.0	31.0	34.0
4	36.40401418703759	37.0	37.0	37.0	35.0	37.0
5	36.37143581115753	37.0	37.0	37.0	35.0	37.0
6	35.24275448228708	37.0	35.0	37.0	32.0	37.0
7	36.255426839151525	37.0	35.0	37.0	35.0	37.0
8	36.52412766068887	37.0	37.0	37.0	35.0	37.0
9	38.66600865349838	39.0	39.0	39.0	38.0	39.0
10	37.84629529620856	39.0	38.0	39.0	35.0	39.0
11	37.29093973198645	39.0	37.0	39.0	34.0	39.0
12	36.595665889743984	38.0	35.0	39.0	33.0	39.0
13	36.42596644062062	38.0	35.0	39.0	33.0	39.0
14	37.03827400008069	39.0	36.0	40.0	33.0	40.0
15	36.989967717482315	39.0	35.0	40.0	33.0	40.0
16	36.86303251821333	39.0	35.0	40.0	32.0	40.0
17	36.63781577377182	38.0	35.0	40.0	30.0	40.0
18	36.51662432874517	38.0	35.0	40.0	30.0	40.0
19	36.46483923504377	38.0	35.0	40.0	30.0	40.0
20	36.129716832561954	38.0	34.0	40.0	30.0	40.0
21	36.35718648763235	38.0	35.0	40.0	30.0	40.0
22	36.26971650697638	38.0	35.0	40.0	30.0	40.0
23	36.301626016835606	38.0	35.0	40.0	31.0	40.0
24	36.32484805416612	38.0	35.0	40.0	31.0	40.0
25	36.202076811299804	38.0	35.0	40.0	31.0	40.0
26	35.97202158207694	38.0	34.0	40.0	30.0	40.0
27	35.780792432824974	38.0	34.0	40.0	30.0	40.0
28	35.546315609917336	38.0	34.0	40.0	29.0	40.0
29	36.11663112357459	38.0	35.0	40.0	30.0	40.0
30	36.27477936268747	38.0	35.0	40.0	31.0	40.0
31	36.26693133470976	38.0	35.0	40.0	31.0	40.0
32	35.55037481269982	38.0	34.0	40.0	29.0	40.0
33	35.68207700948233	38.0	34.0	40.0	29.0	40.0
34	35.44857835889298	38.0	34.0	40.0	27.0	40.0
35	35.43288371852702	38.0	34.0	40.0	28.0	40.0
36	35.377149838021175	38.0	34.0	40.0	28.0	40.0
37	35.38155161345348	37.0	34.0	40.0	29.0	40.0
38	35.21372782036736	37.0	34.0	40.0	28.0	40.0
39	35.168339067650315	37.0	34.0	40.0	28.0	40.0
40	35.00103691928096	37.0	34.0	40.0	28.0	40.0
41	34.8423507561725	36.0	34.0	40.0	28.0	40.0
42	34.71381240183772	36.0	33.0	40.0	27.0	40.0
43	34.61681975087041	36.0	33.0	40.0	27.0	40.0
44	34.454561347754414	35.0	33.0	39.0	27.0	40.0
45	34.304949820892546	35.0	33.0	39.0	26.0	40.0
46	33.949883178479645	35.0	33.0	39.0	25.0	40.0
47	33.83304396325413	35.0	33.0	39.0	24.0	40.0
48	33.70692343572056	35.0	33.0	39.0	24.0	40.0
49	33.40895317867075	35.0	33.0	39.0	24.0	40.0
50	32.93275171480968	35.0	31.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	8.0
15	22.0
16	89.0
17	239.0
18	607.0
19	1220.0
20	2136.0
21	2831.0
22	3670.0
23	5059.0
24	6709.0
25	9151.0
26	11972.0
27	15805.0
28	20560.0
29	26691.0
30	33950.0
31	44280.0
32	60601.0
33	88414.0
34	148239.0
35	127040.0
36	176115.0
37	241365.0
38	335030.0
39	51030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.38697898345104	0.5177518457517098	0.0288780250262061	0.06639114577103265
8	99.37530037038898	0.5255375877930889	0.04437872963586085	0.05478331218206745
9	98.23858203234764	1.0783252727310046	0.2683957620082685	0.414696932913092
10	59.9514877491349	28.50650357188611	4.728564259621939	6.813444419357054
11	37.62750037336172	21.840846692369055	22.67753084392489	17.854122090344333
12	33.902589042346655	14.119513971514094	26.197747938724795	25.780149047414465
13	26.152378296465486	14.60923714591684	29.973478931428136	29.264905626189535
14	25.049492546567585	16.835747031331948	33.09152706005426	25.023233362046206
15	28.121038561364742	18.213681813709844	29.09397319864471	24.5713064262807
16	35.7458280809066	19.212946414984298	23.76866720128762	21.27255830282148
17	34.243179866920435	18.416889680989836	23.99275501313313	23.347175438956597
18	32.895326360611506	19.95917439991393	23.86188376736486	23.2836154721097
19	31.934141115866705	23.270096592746945	21.641602475582854	23.1541598158035
20	31.355377364299823	23.63623880711107	22.90664399836075	22.101739830228357
21	32.625585788614266	21.350486502708378	24.661762592906904	21.362165115770445
22	30.21844668783917	19.4265588648105	27.41600422978131	22.93899021756902
23	32.14471004834946	19.48919869850705	26.20298561973445	22.163105633409046
24	32.584038237902554	19.94784968421738	23.139933141709708	24.328178936170364
25	29.71216111673021	21.80113940795802	21.893152722992497	26.593546752319263
26	27.668049933502687	20.064989712203584	24.970714993003448	27.296245361290282
27	25.941667805036527	19.40865165811533	26.01365052918273	28.63603000766542
28	25.7502093302917	22.313936690592488	22.688714000675237	29.247139978440572
29	30.56434597289571	22.07548064570698	22.719503071475238	24.64067030992208
30	29.896187746799175	19.535700812336014	25.018986593659996	25.549124847204812
31	31.504509714128787	20.286812580909785	23.73108330106969	24.47759440389174
32	35.031167740981104	20.384629812738748	23.096120647858672	21.488081798421476
33	35.051623008708	20.036040907704276	23.881489681414514	21.03084640217321
34	29.117896660553676	22.16076991079663	27.955343814829575	20.765989613820114
35	26.92734274747512	26.28600994168479	27.307853194879243	19.47879411596084
36	27.32215064844614	28.660448925886108	22.360226466002143	21.65717395966561
37	29.225339900724713	26.97547278918546	21.04811659361045	22.751070716479372
38	30.56696481340054	27.53710790826131	20.18793365698427	21.707993621353882
39	31.58732169765982	24.165810824871055	20.64531061217874	23.601556865290384
40	29.069766618843335	23.34328256793591	21.25465109612631	26.332299717094447
41	25.631299815477917	24.43760400158829	22.748593434920753	27.182502748013043
42	24.4110616991745	23.046362678266952	22.207130465679388	30.335445156879164
43	25.13704675479655	20.51528872008771	24.790156557116557	29.557507967999186
44	26.814449487875123	20.72741480097874	23.966778946504167	28.491356764641974
45	28.21821877793577	20.842360665298735	24.010025204570372	26.92939535219512
46	26.854793787544086	24.406036356584153	23.886231906112446	24.852937949759315
47	25.794446500981355	23.36225146672763	24.153707534970366	26.689594497320645
48	27.087870592473735	21.21600550381183	26.123429491966178	25.57269441174826
49	28.40748308901439	20.37585315807392	25.507648075966195	25.709015676945494
50	27.242736079624073	21.046063988890452	25.015589178951036	26.69561075253444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	10.0
10	17.0
11	34.5
12	52.0
13	126.0
14	200.0
15	761.5
16	1323.0
17	2728.0
18	4133.0
19	3610.0
20	3087.0
21	3052.0
22	3017.0
23	3545.0
24	4073.0
25	3898.5
26	3724.0
27	5062.0
28	6400.0
29	6888.5
30	7377.0
31	7791.5
32	8206.0
33	10688.5
34	13171.0
35	15823.0
36	18475.0
37	19989.5
38	21504.0
39	22306.5
40	23109.0
41	25016.0
42	26923.0
43	31044.5
44	35166.0
45	35294.0
46	35422.0
47	40834.0
48	46246.0
49	44088.0
50	41930.0
51	48617.0
52	55304.0
53	61710.5
54	68117.0
55	68100.5
56	68084.0
57	79145.0
58	90206.0
59	104911.5
60	119617.0
61	127404.5
62	135192.0
63	140243.0
64	145294.0
65	145756.0
66	146218.0
67	116503.0
68	86788.0
69	73035.5
70	59283.0
71	50259.5
72	41236.0
73	38407.5
74	35579.0
75	29105.0
76	22631.0
77	21606.5
78	20582.0
79	14853.0
80	9124.0
81	6223.0
82	3322.0
83	2492.0
84	1662.0
85	1132.0
86	602.0
87	456.0
88	310.0
89	200.0
90	90.0
91	62.0
92	34.0
93	18.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1412839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.620297969140129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.60799080442314	5.914213237981534
2	11.02069489833605	1.9000334770127847
3	4.736213321060487	1.2248271021885644
4	2.5561267815411917	0.8813829801513693
5	1.3395318319050689	0.5773581765084911
6	0.9110670179519658	0.47122014987611305
7	0.5711744217444508	0.3446585595451935
8	0.4084593305536422	0.2816832910118318
9	0.32964756291875147	0.2557494195444456
>10	3.6744228753367594	8.155703223868144
>50	2.7515799967783043	17.584746491550472
>100	2.910047954707945	45.18206227750349
>500	0.13558755758685825	7.868469301990172
>1k	0.044913378450646795	6.8520471257636135
>5k	8.47422234917864E-4	0.45532265519765247
>10k+	0.001694844469835728	2.0505225303061296
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	15578	1.1026026320054867	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	12492	0.8841771780082515	No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAG	6233	0.44116845585378095	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4233	0.2996095096468883	No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTG	4130	0.2923192239172333	No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3748	0.2652814651917168	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	3587	0.2538859700220619	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	3445	0.2438352848413726	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3340	0.23640344016551074	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3032	0.21460336244964925	No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATT	2879	0.20377410306482197	No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2707	0.19160003369102918	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2591	0.18338961481102942	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC	2464	0.17440062172689175	No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2336	0.1653408491696506	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2273	0.1608817423641335	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2027	0.1434699919806857	No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2019	0.14290375619585813	No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAA	1812	0.12825240526344472	No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGT	1742	0.1232978421462035	No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1722	0.12188225268413458	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	1715	0.12138679637241044	No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1609	0.11388417222344513	No Hit
TGCGGAGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	1564	0.11069909593379006	No Hit
TGCGGAGGGAACCTTTGATGACTCTGTATTTCGTTTTTGAGACTGTATTC	1531	0.10836337332137633	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCT	1490	0.10546141492413501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007007167837241186	0.0	0.0	0.0	0.0
7	0.007148726783448078	0.0	0.0	0.0	0.0
8	0.007219506256551525	0.0	0.0	0.0	0.0
9	0.0072902857296549715	0.0	0.0	0.0	0.0
10	0.01387277672827548	0.0	0.0	0.0	0.0
11	0.020313708780689096	0.0	0.0	0.0	0.0
12	0.02576372820965446	0.0	0.0	0.0	0.0
13	0.03022283501517158	0.0	0.0	0.0	0.0
14	0.04260924280827469	0.0	0.0	0.0	0.0
15	0.048696277495171074	0.0	0.0	0.0	0.0
16	0.057048255321377736	0.0	0.0	0.0	0.0
17	0.06271061316965344	0.0	0.0	0.0	0.0
18	0.06808985312551537	0.0	0.0	0.0	0.0
19	0.07913145092965299	0.0	0.0	0.0	0.0
20	0.0854308240358597	0.0	0.0	0.0	0.0
21	0.09059772557241129	0.0	0.0	0.0	0.0
22	0.0942782581737905	0.0	0.0	0.0	0.0
23	0.10078996969930756	0.0	0.0	0.0	0.0
24	0.10708934280551428	0.0	0.0	0.0	0.0
25	0.11190234697654863	0.0	0.0	0.0	0.0
26	0.11756470482482434	0.0	0.0	0.0	0.0
27	0.12202381163034146	0.0	0.0	0.0	0.0
28	0.12881864104827231	2.1233841931033896E-4	0.0	0.0	0.0
29	0.13285307101516874	2.1233841931033896E-4	0.0	0.0	0.0
30	0.13894010570206514	2.1233841931033896E-4	0.0	0.0	0.0
31	0.14502714038896153	2.1233841931033896E-4	0.0	0.0	0.0
32	0.1520343082262027	2.1233841931033896E-4	0.0	0.0	0.0
33	0.1578382250206853	2.1233841931033896E-4	0.0	0.0	0.0
34	0.16413759812689202	2.1233841931033896E-4	0.0	0.0	0.0
35	0.17319737068413316	2.1233841931033896E-4	0.0	0.0	0.0
36	0.18515910163861557	2.1233841931033896E-4	0.0	0.0	0.0
37	0.19513900734620152	2.1233841931033896E-4	0.0	0.0	0.0
38	0.20193383676413237	2.1233841931033896E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.857094E-4	44.000004	10
CGAACGC	20	7.857094E-4	44.000004	27
GTCGATA	20	7.857094E-4	44.000004	44
TTTACGG	20	7.857094E-4	44.000004	13
CGAAAAC	20	7.857094E-4	44.000004	13
CAACGAA	20	7.857094E-4	44.000004	19
CGCATTA	20	7.857094E-4	44.000004	19
CCAATCG	30	2.5281297E-6	44.000004	28
CGTCTTA	40	8.314601E-9	44.000004	16
TTCGTAC	20	7.857094E-4	44.000004	19
CGACACG	20	7.857094E-4	44.000004	38
CGTTATG	20	7.857094E-4	44.000004	24
GTAATCG	20	7.857094E-4	44.000004	44
TTATACG	30	2.5281297E-6	44.000004	41
CGTTAAC	20	7.857094E-4	44.000004	25
AAACGTA	30	2.5281297E-6	44.000004	35
GTTACGT	65	0.0	44.000004	44
ACGATAT	20	7.857094E-4	44.000004	26
TACGCGA	20	7.857094E-4	44.000004	14
CGCTATC	20	7.857094E-4	44.000004	33
>>END_MODULE