FastQCFastQC Report
Fri 17 Jun 2016
SRR1527146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568075
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC149410.9528243228161919No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT98130.6257991486376608No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTG60920.38850182548666357No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44980.28684852446470993No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTGCA39900.2544521148542002No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA35920.2290706758286434No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT35780.22817786139055848No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT34100.2174640881335395No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC33770.21535959695805368No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATTTG30700.19578145178004877No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC29790.18997815793249687No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG25530.1628110900307702No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT24960.15917605981856736No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT22860.14578384324729365No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22760.14514611864866156No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC22380.14272276517385968No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG20400.13009581812094445No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20280.12933054860258597No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC19040.12142276357954818No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC17250.11000749326403392No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGA17070.10885958898649618No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT16810.10720150503005278No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16690.10643623551169427No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16480.1050970138545669No Hit
TGCGGAGGGAACCTTTGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCC16190.10324761251853387No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGTCT15740.10037785182468952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG351.0198892E-746.00000434
ATACCGT351.0198892E-746.00000430
AATCGTC351.0198892E-746.00000432
AATCGAA351.0198892E-746.00000446
TCGTTGA301.8618393E-646.031
GATACAC206.312162E-446.09
AAATCGT453.110472E-1046.031
GTCGACA301.8618393E-646.010
CGAAACT301.8618393E-646.018
GACACGC206.312162E-446.045
CGCATTA301.8618393E-646.019
ATTACGA253.417729E-546.041
ACCGATT301.8618393E-646.044
GCACGTT253.417729E-546.025
AGGCGTA253.417729E-546.036
TATTGCG253.417729E-546.045
CGCCTAT405.6134013E-946.041
TACGACA253.417729E-546.034
TTAGTCG206.312162E-446.018
CGTAAGG206.312162E-446.033
Produced by FastQC (version 0.11.5)