##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568075
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.194420547486565	33.0	31.0	34.0	31.0	34.0
2	32.61514723466671	34.0	31.0	34.0	31.0	34.0
3	32.831610095180395	34.0	31.0	34.0	31.0	34.0
4	36.371139135564306	37.0	37.0	37.0	35.0	37.0
5	36.346999984056886	37.0	37.0	37.0	35.0	37.0
6	35.143400028697606	37.0	35.0	37.0	32.0	37.0
7	36.25768601629386	37.0	35.0	37.0	35.0	37.0
8	36.53918148047766	37.0	37.0	37.0	35.0	37.0
9	38.65368301898825	39.0	39.0	39.0	38.0	39.0
10	37.92071935334726	39.0	38.0	39.0	35.0	39.0
11	37.5205120928527	39.0	37.0	39.0	35.0	39.0
12	36.92165553305805	39.0	35.0	39.0	34.0	39.0
13	36.841742263603464	39.0	35.0	39.0	33.0	39.0
14	37.48210767979848	40.0	36.0	40.0	33.0	40.0
15	37.40189786840553	40.0	36.0	40.0	33.0	40.0
16	32.47544983498876	38.0	33.0	40.0	3.0	40.0
17	33.89189292603989	38.0	33.0	40.0	16.0	40.0
18	35.58127640578416	38.0	33.0	40.0	27.0	40.0
19	36.67964542512316	38.0	35.0	40.0	31.0	40.0
20	37.00874830604403	39.0	35.0	40.0	32.0	40.0
21	37.2555228544553	39.0	35.0	40.0	33.0	40.0
22	37.30021331887824	39.0	35.0	40.0	33.0	40.0
23	37.19973917063916	39.0	35.0	40.0	33.0	40.0
24	37.162566841509495	39.0	35.0	40.0	33.0	40.0
25	37.06196195972769	39.0	35.0	40.0	32.0	40.0
26	36.912081373658786	39.0	35.0	40.0	32.0	40.0
27	36.742519968751495	39.0	35.0	40.0	32.0	40.0
28	36.6411644851171	38.0	35.0	40.0	31.0	40.0
29	36.39448814629402	38.0	35.0	40.0	31.0	40.0
30	36.34549304083032	38.0	35.0	40.0	31.0	40.0
31	36.2598115523811	38.0	35.0	40.0	31.0	40.0
32	36.15649506560592	38.0	35.0	40.0	31.0	40.0
33	35.75791719146087	38.0	35.0	40.0	30.0	40.0
34	35.44114280248075	38.0	34.0	40.0	28.0	40.0
35	35.34448479823988	38.0	34.0	40.0	28.0	40.0
36	35.38315067837954	37.0	34.0	40.0	29.0	40.0
37	35.15633180810867	37.0	34.0	40.0	28.0	40.0
38	35.13806801332844	37.0	34.0	40.0	28.0	40.0
39	34.75177143950385	36.0	34.0	40.0	27.0	40.0
40	34.690011638473926	36.0	33.0	39.0	27.0	40.0
41	34.58417040001275	36.0	33.0	39.0	27.0	40.0
42	34.50253527414186	35.0	33.0	39.0	27.0	40.0
43	34.412359740446085	35.0	33.0	39.0	27.0	40.0
44	34.80262104810038	35.0	34.0	39.0	28.0	40.0
45	34.89913492658196	35.0	34.0	39.0	29.0	40.0
46	34.81674537251088	35.0	34.0	39.0	29.0	40.0
47	34.52602394655868	35.0	34.0	39.0	28.0	40.0
48	34.457261929435774	35.0	33.0	39.0	28.0	40.0
49	34.37446933341836	35.0	33.0	39.0	28.0	40.0
50	33.902206845973566	35.0	33.0	39.0	26.0	40.0
51	33.92122060488178	35.0	33.0	38.0	27.0	40.0
52	33.59136520893452	35.0	33.0	37.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	7.0
16	51.0
17	140.0
18	324.0
19	628.0
20	1276.0
21	2092.0
22	3551.0
23	5349.0
24	7364.0
25	10123.0
26	13381.0
27	17255.0
28	22153.0
29	27551.0
30	34802.0
31	46194.0
32	63372.0
33	94323.0
34	155911.0
35	168848.0
36	220348.0
37	276604.0
38	318569.0
39	77855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.39473558343829	0.5147075235559523	0.030164373515297417	0.06039251949045805
8	99.3940340863798	0.4960859652758956	0.06058383687004767	0.04929611147425984
9	98.31009358608485	1.0357285206383622	0.24845750362705865	0.4057203896497298
10	60.784975208456224	27.690894887043033	4.766289877716308	6.757840026784432
11	37.585829759418395	21.579898920651118	21.961130685713375	18.873140634217116
12	33.6780447363806	13.996141766178278	26.86516907673421	25.460644420706917
13	26.107169618800118	14.517928032779045	30.41034389298981	28.96455845543102
14	26.272850469524734	16.37472697415621	32.46585781930074	24.88656473701832
15	27.728201776063006	18.031918116161535	28.85397701002822	25.385903097747235
16	33.99926661671157	26.860449914704336	20.817499162986465	18.32278430559763
17	34.90572836120721	18.09824147441927	23.684007461377803	23.312022702995712
18	32.60966471629227	20.822537187315657	23.63362721808587	22.934170878306205
19	32.78835514882898	22.986081660634856	21.312118361685506	22.91344482885066
20	32.081054796486136	23.36724965323725	22.67429810436363	21.877397445912983
21	33.376082138928304	21.121438706694516	24.23914672448703	21.263332429890152
22	30.593498397716946	19.257943657031714	27.30239306155637	22.84616488369498
23	32.408207515584394	19.248632877891684	25.871402834685842	22.47175677183808
24	32.590915613092484	19.797968847153356	23.22771551105655	24.383400028697608
25	29.687227970600894	21.599732155668576	21.838623790316152	26.874416083414378
26	27.570173620521977	19.955231733176028	25.070803373563127	27.40379127273887
27	26.096519618002965	19.201505030052772	25.91087798734117	28.791097364603097
28	26.012276198523665	22.32520765907243	22.452369944039667	29.210146198364235
29	30.777737034261754	21.971908231430255	22.58986336750474	24.660491366803246
30	30.083254946351417	19.35628079014078	24.928080608389266	25.632383655118534
31	31.732346985954113	20.14654911276565	23.509334693812477	24.611769207467756
32	35.09168885416833	19.951660475423687	23.126062210034597	21.830588460373388
33	35.46029367217767	19.99776796390479	23.65868979481211	20.88324856910543
34	29.234953685251025	21.995440269119783	27.89943083079572	20.870175214833473
35	27.179949938619007	25.93778996540344	27.19136520893452	19.69089488704303
36	27.396266122475012	28.45686590246002	22.493184318352117	21.65368365671285
37	29.472251008402022	26.829584044130545	20.973869234571048	22.724295712896385
38	30.599429236484227	27.438292173524864	20.229708400427278	21.73257018956364
39	31.806578129234893	24.077993718412706	20.483777880522297	23.63165027183011
40	29.078200978907258	23.297992761825807	21.047271335873603	26.57653492339333
41	25.87012738548858	24.36854104554948	22.53393492020471	27.227396648757235
42	24.462414106468124	22.84482566203785	22.16246034150152	30.530299889992506
43	25.44954801269072	20.26816319372479	24.67209795449835	29.610190839086144
44	26.808475359915818	20.65405034835706	23.942987420882293	28.594486870844825
45	28.33512427658116	20.68874256652265	23.94828053505094	27.027852621845255
46	26.684246608102292	24.366691644213446	23.83616855061142	25.112893197072843
47	26.174449563955804	23.22293257656681	24.207834446694196	26.39478341278319
48	27.199272993957557	21.175517752658514	26.043907338615817	25.581301914768105
49	28.324920683003047	20.192529056327025	25.615483953254785	25.867066307415143
50	27.111713406565375	21.283484527206927	24.957352167466478	26.647449898761224
51	23.64255536246672	21.48137047016246	27.438100856145276	27.437973311225548
52	24.91545366133635	21.66981808905824	26.245045677024375	27.16968257258103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	12.0
10	20.0
11	39.0
12	58.0
13	125.5
14	754.5
15	1316.0
16	2724.0
17	4132.0
18	3736.5
19	3341.0
20	3179.5
21	3018.0
22	3556.5
23	4095.0
24	3950.5
25	3806.0
26	4171.5
27	4537.0
28	5987.0
29	7437.0
30	7999.5
31	8562.0
32	9776.0
33	10990.0
34	11667.5
35	12345.0
36	17316.0
37	22287.0
38	22451.5
39	23774.0
40	24932.0
41	26666.5
42	28401.0
43	33997.0
44	39593.0
45	38567.5
46	37542.0
47	41476.5
48	45411.0
49	46220.0
50	47029.0
51	53888.0
52	60747.0
53	66122.5
54	71498.0
55	74895.5
56	78293.0
57	88578.5
58	98864.0
59	113630.0
60	128396.0
61	138320.5
62	148245.0
63	152478.0
64	156018.5
65	155326.0
66	126289.5
67	97253.0
68	85766.5
69	74280.0
70	62462.5
71	50645.0
72	46457.0
73	42269.0
74	35641.5
75	29014.0
76	26978.5
77	24943.0
78	18655.5
79	12368.0
80	8556.0
81	4744.0
82	3230.0
83	1716.0
84	1264.5
85	813.0
86	501.5
87	190.0
88	126.0
89	40.5
90	19.0
91	13.5
92	8.0
93	5.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1568075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.092648922180068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05421818460502	7.373105764215613
2	9.934975736366635	2.0054044431505167
3	3.9415554490967155	1.193422060651168
4	1.9691104198137201	0.7949416062474594
5	1.08814599597261	0.549113775671376
6	0.7362312739495048	0.44583142641010376
7	0.47337196090159644	0.3344303907688634
8	0.35766003024627513	0.28877896950175674
9	0.27566280305005403	0.2503951102879438
>10	3.1821704585056314	8.48145816976297
>50	2.401573693548091	17.872251827126227
>100	2.439320848280609	44.13810846007522
>500	0.10327051766443453	6.994443048662687
>1k	0.04059599659912254	7.061076644803832
>5k	0.001424420933302545	1.14326574608945
>10k+	7.122104666512725E-4	1.0739725565748177
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14941	0.9528243228161919	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9813	0.6257991486376608	No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTG	6092	0.38850182548666357	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4498	0.28684852446470993	No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTGCA	3990	0.2544521148542002	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3592	0.2290706758286434	No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3578	0.22817786139055848	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3410	0.2174640881335395	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC	3377	0.21535959695805368	No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATTTG	3070	0.19578145178004877	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2979	0.18997815793249687	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2553	0.1628110900307702	No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2496	0.15917605981856736	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	2286	0.14578384324729365	No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2276	0.14514611864866156	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	2238	0.14272276517385968	No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2040	0.13009581812094445	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2028	0.12933054860258597	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1904	0.12142276357954818	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1725	0.11000749326403392	No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGA	1707	0.10885958898649618	No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1681	0.10720150503005278	No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1669	0.10643623551169427	No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1648	0.1050970138545669	No Hit
TGCGGAGGGAACCTTTGATGACTCTGTATTTCGTTTTTGAGACTGTATTCCC	1619	0.10324761251853387	No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGTCT	1574	0.10037785182468952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.006185928606731183	0.0	0.0	0.0	0.0
7	0.006313473526457599	0.0	0.0	0.0	0.0
8	0.006377245986320807	0.0	0.0	0.0	0.0
9	0.006568563365910432	0.0	0.0	0.0	0.0
10	0.011415270315514245	0.0	0.0	0.0	0.0
11	0.017409881542655804	0.0	0.0	0.0	0.0
12	0.02193772619294358	0.0	0.0	0.0	0.0
13	0.025381439025556812	0.0	0.0	0.0	0.0
14	0.03507485292476444	0.0	0.0	0.0	0.0
15	0.03966647003491542	0.0	0.0	0.0	0.0
16	0.04935988393412305	0.0	0.0	0.0	0.0
17	0.05560958500071744	0.0	0.0	0.0	0.0
18	0.06026497457073163	0.0	0.0	0.0	0.0
19	0.07276437670392041	0.0	0.0	0.0	0.0
20	0.07984311974873651	0.0	0.0	0.0	0.0
21	0.08666677295409977	0.0	0.0	0.0	0.0
22	0.0900467133268498	0.0	0.0	0.0	0.0
23	0.09585000717440173	0.0	0.0	0.0	0.0
24	0.10292875021921782	0.0	0.0	0.0	0.0
25	0.10828563684772731	0.0	0.0	6.377245986320807E-5	0.0
26	0.11581078711158586	0.0	0.0	6.377245986320807E-5	0.0
27	0.12110390128023213	0.0	0.0	6.377245986320807E-5	0.0
28	0.12767246464614257	0.0	0.0	6.377245986320807E-5	0.0
29	0.13315689619437845	0.0	0.0	6.377245986320807E-5	0.0
30	0.13953414218069926	0.0	0.0	6.377245986320807E-5	0.0
31	0.14552875340784083	0.0	0.0	6.377245986320807E-5	0.0
32	0.15260749645265692	0.0	0.0	6.377245986320807E-5	0.0
33	0.15694402372335506	0.0	0.0	6.377245986320807E-5	0.0
34	0.16402276676817115	0.0	0.0	6.377245986320807E-5	0.0
35	0.17237695901025143	0.0	0.0	6.377245986320807E-5	0.0
36	0.18200660044959585	0.0	0.0	6.377245986320807E-5	0.0
37	0.1920188766481195	0.0	0.0	6.377245986320807E-5	0.0
38	0.19999043413102052	0.0	0.0	6.377245986320807E-5	0.0
39	0.20891857851186965	0.0	0.0	6.377245986320807E-5	0.0
40	0.21593354909682252	0.0	0.0	6.377245986320807E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	35	1.0198892E-7	46.000004	34
ATACCGT	35	1.0198892E-7	46.000004	30
AATCGTC	35	1.0198892E-7	46.000004	32
AATCGAA	35	1.0198892E-7	46.000004	46
TCGTTGA	30	1.8618393E-6	46.0	31
GATACAC	20	6.312162E-4	46.0	9
AAATCGT	45	3.110472E-10	46.0	31
GTCGACA	30	1.8618393E-6	46.0	10
CGAAACT	30	1.8618393E-6	46.0	18
GACACGC	20	6.312162E-4	46.0	45
CGCATTA	30	1.8618393E-6	46.0	19
ATTACGA	25	3.417729E-5	46.0	41
ACCGATT	30	1.8618393E-6	46.0	44
GCACGTT	25	3.417729E-5	46.0	25
AGGCGTA	25	3.417729E-5	46.0	36
TATTGCG	25	3.417729E-5	46.0	45
CGCCTAT	40	5.6134013E-9	46.0	41
TACGACA	25	3.417729E-5	46.0	34
TTAGTCG	20	6.312162E-4	46.0	18
CGTAAGG	20	6.312162E-4	46.0	33
>>END_MODULE