FastQCFastQC Report
Fri 17 Jun 2016
SRR1527145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519094
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC141690.9327270070186572No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT98080.6456479980830679No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTG56860.37430205109097925No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG41810.27522984094466835No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTGCA39200.258048547357833No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT36340.23922153599448093No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA34240.22539750667173986No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT33670.22164527014128158No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC31230.2055830646424777No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC27900.183662103859274No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATTTG27370.18017318217305842No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT25510.16792904191577349No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG25030.16476926378486123No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22240.14640305339893384No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT21940.1444281920671137No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20550.1352780012296803No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC19930.13119662114391867No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG19380.12757604203558173No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17860.11757007795435964No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGA16970.11171132266995987No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC16910.11131635040359583No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16640.10953897520495769No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16390.1078932574284409No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGTCT15820.1041410208979826No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT15760.1037460486316186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT551.8189894E-1246.00000437
GATACAC301.8618011E-646.09
CGTATAG253.4176785E-546.034
ATTACGA301.8618011E-646.041
ATTCGAC206.3120987E-446.044
CTACGAC206.3120987E-446.033
TACGACA206.3120987E-446.034
CGCTAAT206.3120987E-446.037
TTACGCG453.110472E-1046.016
CCGAATT453.110472E-1046.025
GCGTATA206.3120987E-446.040
TACGTCA301.8618011E-646.031
ATCGAAC650.046.041
ATATACG351.0198528E-745.99999632
GTACGTC5200.045.11538723
AGGGATT56150.043.9928746
AGGGATC46800.043.8867536
TGCGGAG1599400.043.4014631
CGGAGGG1568700.043.271433
GCGGAGG1595800.043.2399442
Produced by FastQC (version 0.11.5)