##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1519094
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16878152372401	33.0	31.0	34.0	31.0	34.0
2	32.608240174735734	34.0	31.0	34.0	31.0	34.0
3	32.832049892896684	34.0	31.0	34.0	31.0	34.0
4	36.37913848649261	37.0	37.0	37.0	35.0	37.0
5	36.362131638990085	37.0	37.0	37.0	35.0	37.0
6	35.144709938950456	37.0	35.0	37.0	32.0	37.0
7	36.26860088974086	37.0	35.0	37.0	35.0	37.0
8	36.56918531703766	37.0	37.0	37.0	35.0	37.0
9	38.6002321120352	39.0	39.0	39.0	38.0	39.0
10	37.910538123381436	39.0	38.0	39.0	35.0	39.0
11	37.43692556221011	39.0	37.0	39.0	35.0	39.0
12	36.878727715335586	39.0	35.0	39.0	34.0	39.0
13	36.74105684045885	39.0	35.0	39.0	33.0	39.0
14	37.393744560902746	40.0	36.0	40.0	33.0	40.0
15	37.51404784694035	40.0	36.0	40.0	33.0	40.0
16	37.60797620160438	40.0	36.0	40.0	33.0	40.0
17	37.4495238609329	40.0	36.0	40.0	33.0	40.0
18	37.24575306070592	40.0	36.0	40.0	32.0	40.0
19	37.333111710006094	40.0	36.0	40.0	32.0	40.0
20	37.30022961054418	39.0	35.0	40.0	33.0	40.0
21	33.09490327787484	38.0	33.0	40.0	10.0	40.0
22	33.676351825495985	38.0	33.0	40.0	16.0	40.0
23	35.59860416801067	38.0	33.0	40.0	28.0	40.0
24	36.450405307374	38.0	35.0	40.0	31.0	40.0
25	36.73075662203919	38.0	35.0	40.0	31.0	40.0
26	36.76984044437013	39.0	35.0	40.0	32.0	40.0
27	36.700160095425296	39.0	35.0	40.0	32.0	40.0
28	36.60561690059996	38.0	35.0	40.0	31.0	40.0
29	36.545936591152355	38.0	35.0	40.0	31.0	40.0
30	36.42598680529316	38.0	35.0	40.0	31.0	40.0
31	36.2404143522389	38.0	35.0	40.0	31.0	40.0
32	36.16526034596937	38.0	35.0	40.0	31.0	40.0
33	35.779280939823344	38.0	35.0	40.0	30.0	40.0
34	35.46098003151879	38.0	34.0	40.0	28.0	40.0
35	35.35620837156884	38.0	34.0	40.0	28.0	40.0
36	35.331808959814204	37.0	34.0	40.0	29.0	40.0
37	35.312374349447765	37.0	34.0	40.0	29.0	40.0
38	35.23265380549196	37.0	34.0	40.0	29.0	40.0
39	35.052938132860774	36.0	34.0	40.0	29.0	40.0
40	30.337219421576282	35.0	28.0	39.0	8.0	40.0
41	31.05237332251987	35.0	28.0	39.0	12.0	40.0
42	32.69060308315351	35.0	30.0	39.0	22.0	40.0
43	33.574888716563954	35.0	32.0	38.0	26.0	40.0
44	34.457529290484985	35.0	33.0	39.0	27.0	40.0
45	34.75744884780007	35.0	33.0	39.0	28.0	40.0
46	34.7834551383917	35.0	34.0	39.0	29.0	40.0
47	34.73448910995633	35.0	34.0	39.0	29.0	40.0
48	34.57580636879614	35.0	34.0	39.0	28.0	40.0
49	34.44500274505725	35.0	34.0	39.0	28.0	40.0
50	34.350644528909996	35.0	34.0	39.0	28.0	40.0
51	34.134685542830134	35.0	33.0	38.0	27.0	40.0
52	33.7419909498688	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	5.0
15	19.0
16	55.0
17	136.0
18	321.0
19	680.0
20	1371.0
21	2314.0
22	3728.0
23	5586.0
24	7595.0
25	10421.0
26	13711.0
27	17976.0
28	22417.0
29	29130.0
30	37735.0
31	52123.0
32	72275.0
33	90220.0
34	167400.0
35	177591.0
36	196239.0
37	217547.0
38	313309.0
39	79186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.40220947485804	0.5176111550700615	0.02712142895699674	0.05305794111490138
8	99.41037223502957	0.4924645874448849	0.044566037388074735	0.05259714013747668
9	98.23335488126476	1.0556950392799918	0.2976774314163574	0.4132726480388969
10	60.83343097925474	27.6741926437732	4.767051940169601	6.725324436802463
11	37.66870252927074	21.56561740089817	21.919380894138214	18.84629917569288
12	33.65538933074583	14.02941490125035	26.881417476469526	25.4337782915343
13	26.319701084988818	14.501999217954914	30.343678534705553	28.834621162350714
14	26.236954395185553	16.381869719714516	32.53070580227425	24.850470082825684
15	27.65589226209833	17.964589419746243	28.946003341465378	25.433514976690052
16	36.32184710096939	18.878028614424124	23.594194960943828	21.205929323662655
17	34.961825930455916	18.078933890858632	23.649096105968425	23.310144072717026
18	32.596271198490676	20.829323267684554	23.65245337023252	22.921952163592245
19	32.8002743740677	22.949205249971364	21.271297233745905	22.97922314221503
20	32.00631428996494	23.420933793432138	22.726111748186746	21.846640168416172
21	37.36957686621105	20.23706235427169	23.106865012961674	19.286495766555593
22	30.78920725116418	19.329811058433513	27.154474969949195	22.726506720453113
23	32.32821668705162	19.322833215061085	25.8415213278441	22.507428770043198
24	32.72621707412445	19.766123755343646	23.191125763119334	24.316533407412578
25	29.743978976942838	21.643756080927183	21.83057796291737	26.78168697921261
26	27.607376502046616	19.961042568794294	25.044335636899362	27.387245292259728
27	26.065207288028258	19.25489798524647	25.908995756681286	28.770898970043984
28	26.054082235859006	22.33015205115681	22.477213391666346	29.13855232131784
29	30.63839367412418	21.968949913566902	22.633819895279686	24.758836517029227
30	30.018089729799474	19.396561371449035	24.947304116795934	25.63804478195556
31	31.733651768751635	20.156422183222368	23.61242951390763	24.497496534118362
32	35.11303447976228	19.99968402218691	23.093304298483176	21.79397719956764
33	35.43256704325078	20.044184230863923	23.647845360458273	20.875403365427026
34	29.177786233109998	21.976586044049938	27.86187029900717	20.98375742383289
35	27.198909349915144	26.03170047409838	27.10253611692232	19.666854059064153
36	27.471176898862087	28.432210251636832	22.493538912009395	21.60307393749169
37	29.326559120107117	26.834876577749633	20.96493041246954	22.873633889673712
38	30.48323540215418	27.386060375460637	20.30644581572964	21.82425840665555
39	31.538601297878866	24.15531889402499	20.536517160886685	23.769562647209455
40	29.294829681375873	27.597304709254335	19.584239026683008	23.523626582686784
41	25.770163005054332	24.415605617558885	22.507560427465318	27.306670949921468
42	24.44529436624725	22.87811024202584	22.18993689659758	30.486658495129333
43	25.376178169356205	20.339294342548914	24.73329497713769	29.55123251095719
44	26.832243429307205	20.648228483556647	23.927354067621884	28.59217401951426
45	28.29989454240488	20.692136233834113	23.937820832680533	27.070148391080473
46	26.5907178884256	24.397107749750838	23.884367919299265	25.127806442524292
47	25.9519819050039	23.285458306069277	24.232996773076586	26.52956301585024
48	27.144929806845397	21.32685666588111	26.017349815087147	25.510863712186342
49	28.360786100136004	20.308552334483583	25.542066521229103	25.788595044151318
50	27.083643276847912	21.226467881513585	25.08830921588789	26.60157962575061
51	23.669700492530417	21.641847048306424	27.325366303862697	27.363086155300465
52	24.962642206473067	21.78739432846157	26.161185548754716	27.08877791631064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	8.5
10	15.0
11	42.0
12	69.0
13	131.5
14	699.0
15	1204.0
16	2533.0
17	3862.0
18	3526.5
19	3191.0
20	3129.0
21	3067.0
22	3462.0
23	3857.0
24	3836.0
25	3815.0
26	4148.5
27	4482.0
28	5749.0
29	7016.0
30	7644.5
31	8273.0
32	9501.0
33	10729.0
34	11254.0
35	11779.0
36	16416.0
37	21053.0
38	21265.0
39	22573.0
40	23669.0
41	25559.5
42	27450.0
43	32677.0
44	37904.0
45	37392.5
46	36881.0
47	40529.0
48	44177.0
49	44616.5
50	45056.0
51	51994.0
52	58932.0
53	64364.5
54	69797.0
55	72849.5
56	75902.0
57	85537.0
58	95172.0
59	108823.5
60	122475.0
61	132585.5
62	142696.0
63	145949.5
64	151255.0
65	153307.0
66	125122.0
67	96937.0
68	84692.0
69	72447.0
70	61018.5
71	49590.0
72	45253.5
73	40917.0
74	34438.5
75	27960.0
76	26060.0
77	24160.0
78	18360.0
79	12560.0
80	8759.5
81	4959.0
82	3387.5
83	1816.0
84	1306.0
85	796.0
86	487.5
87	179.0
88	109.5
89	28.5
90	17.0
91	12.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1519094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.915859878546398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5673644485076	7.195678176268107
2	9.241595443304208	1.832767309400348
3	3.878258155481953	1.1536879332776653
4	2.109065036048934	0.8365277348881053
5	1.239365731519835	0.6144688466011418
6	0.8444611521491717	0.5024135074552222
7	0.5312033549276317	0.36871366241333015
8	0.3793285651241842	0.3009095119761178
9	0.310946854839903	0.27749748980404665
>10	3.744725909446696	9.547490002191804
>50	2.5678301088653384	18.69542332383192
>100	2.4416541924308905	43.02389326944199
>500	0.10289345566380562	6.781193474168506
>1k	0.039054450324948115	6.66024967911876
>5k	0.0015020942432672352	1.1538812564659862
>10k+	7.510471216336176E-4	1.0552048226969508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14169	0.9327270070186572	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	9808	0.6456479980830679	No Hit
TGCGGAGGGGTTTGGGTGAGTTCCCTTCTGTGTCTATGTGTGTGTTAGAGTG	5686	0.37430205109097925	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4181	0.27522984094466835	No Hit
TGCGGAGGGAAGTTATGACCTGGTACGTCTGAGCTGCACAGTGATTGCTGCA	3920	0.258048547357833	No Hit
TGCGGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3634	0.23922153599448093	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3424	0.22539750667173986	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3367	0.22164527014128158	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC	3123	0.2055830646424777	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2790	0.183662103859274	No Hit
TGCGGAGGGATGGTAACAAACCTGACAAGTTCAGACCCCAGTATATTATTTG	2737	0.18017318217305842	No Hit
TGCGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2551	0.16792904191577349	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2503	0.16476926378486123	No Hit
TGCGGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2224	0.14640305339893384	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	2194	0.1444281920671137	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2055	0.1352780012296803	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	1993	0.13119662114391867	No Hit
TGCGGAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1938	0.12757604203558173	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1786	0.11757007795435964	No Hit
TGCGGAGGGAGATGGGGTCAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGA	1697	0.11171132266995987	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1691	0.11131635040359583	No Hit
TGCGGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1664	0.10953897520495769	No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1639	0.1078932574284409	No Hit
TGCGGAGGGCTGCTCTGTGGGGTTTGTCATTTTGTTAGGCCCTTGCCTGTCT	1582	0.1041410208979826	No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1576	0.1037460486316186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.008689389860008664	0.0	0.0	0.0	0.0
7	0.008821047282130007	0.0	0.0	0.0	0.0
8	0.008886875993190678	0.0	0.0	0.0	0.0
9	0.009084362126372694	0.0	0.0	0.0	0.0
10	0.013955686744862398	0.0	0.0	0.0	0.0
11	0.02099935882835427	0.0	0.0	0.0	0.0
12	0.026660627979572034	0.0	0.0	0.0	0.0
13	0.030083720954726962	0.0	0.0	0.0	0.0
14	0.039168083081099654	0.0	0.0	0.0	0.0
15	0.046540898719894884	0.0	0.0	0.0	0.0
16	0.054177029202932804	0.0	0.0	0.0	0.0
17	0.05924583995460452	0.0	0.0	0.0	0.0
18	0.06319556261824483	0.0	0.0	0.0	0.0
19	0.07366232767689163	0.0	0.0	0.0	0.0
20	0.08103514331568686	0.0	0.0	0.0	0.0
21	0.08847378766554276	0.0	0.0	0.0	0.0
22	0.09262099646236507	0.0	0.0	0.0	0.0
23	0.09768980721403679	0.0	0.0	0.0	0.0
24	0.10637919707404545	0.0	0.0	0.0	0.0
25	0.11190880880314187	0.0	0.0	0.0	0.0
26	0.1188208234645124	0.0	0.0	0.0	0.0
27	0.12421877777148747	0.0	0.0	0.0	0.0
28	0.13119662114391867	0.0	0.0	0.0	0.0
29	0.1352780012296803	0.0	0.0	0.0	0.0
30	0.14192670104680816	0.0	0.0	0.0	0.0
31	0.1469955117984799	0.0	0.0	0.0	0.0
32	0.1535125541934864	0.0	0.0	0.0	0.0
33	0.15891050850046146	0.0	0.0	0.0	0.0
34	0.1648350924959219	0.0	0.0	0.0	0.0
35	0.1729978526674452	0.0	0.0	0.0	0.0
36	0.1828063306154853	0.0	0.0	0.0	0.0
37	0.19050828980958387	0.0	0.0	0.0	0.0
38	0.19847356384792514	0.0	0.0	0.0	0.0
39	0.2069654675747518	0.0	0.0	0.0	0.0
40	0.21499657032415373	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	55	1.8189894E-12	46.000004	37
GATACAC	30	1.8618011E-6	46.0	9
CGTATAG	25	3.4176785E-5	46.0	34
ATTACGA	30	1.8618011E-6	46.0	41
ATTCGAC	20	6.3120987E-4	46.0	44
CTACGAC	20	6.3120987E-4	46.0	33
TACGACA	20	6.3120987E-4	46.0	34
CGCTAAT	20	6.3120987E-4	46.0	37
TTACGCG	45	3.110472E-10	46.0	16
CCGAATT	45	3.110472E-10	46.0	25
GCGTATA	20	6.3120987E-4	46.0	40
TACGTCA	30	1.8618011E-6	46.0	31
ATCGAAC	65	0.0	46.0	41
ATATACG	35	1.0198528E-7	45.999996	32
GTACGTC	520	0.0	45.115387	23
AGGGATT	5615	0.0	43.992874	6
AGGGATC	4680	0.0	43.886753	6
TGCGGAG	159940	0.0	43.401463	1
CGGAGGG	156870	0.0	43.27143	3
GCGGAGG	159580	0.0	43.239944	2
>>END_MODULE