FastQCFastQC Report
Fri 17 Jun 2016
SRR1527144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747609
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT128310.7342031312496101No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC74350.42543841328352056No Hit
TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTC61800.35362601131031024No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG60580.3466450447439902No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC53340.3052170136455008No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG50140.28690628166826787No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG40850.23374793789686363No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40320.23071522291313448No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC31460.18001738375117088No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC31110.17801464744116102No Hit
TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA30870.17664134254286856No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA28520.16319439874708816No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC27200.1556412218064796No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA26840.15358126445904088No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG25360.14511255091957068No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG24640.1409926362246933No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT24280.13893267887725458No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC23250.13303891202208273No Hit
TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG22930.13120783882435946No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT22120.12657293479262238No Hit
TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAG21480.12291078839717579No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC21110.12079361001230825No Hit
TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA21030.12033584171287744No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG20570.11770367399115018No Hit
TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATG20360.1165020322051443No Hit
TGATCAGGGGGGGGACTGTTCTGAGGCATCACGTGCAACGATGCTCTGAA20020.11455651693256327No Hit
TGATCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA19900.11386986448341704No Hit
TGATCAGGGGGTTATCTATCTTTCTAGCTTGTCATGGACTTCACACCCAA19230.11003605497568392No Hit
TGATCAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT18940.10837664489024719No Hit
TGATCAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC18550.10614502443052193No Hit
TGATCAGGGAGAGCAAGTGGGGTTAACCAGAGCAAGCAGAGCACCTAAAA17890.10236843596021766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.8576064E-444.00000439
TCGCAAA207.8576064E-444.00000432
ATATGTC207.8576064E-444.00000410
CGTCTAA207.8576064E-444.00000424
CCGTCTA207.8576064E-444.00000435
CATTACG207.8576064E-444.00000421
GTTACGA207.8576064E-444.00000441
CGGATTA207.8576064E-444.00000440
AATTGCG207.8576064E-444.00000432
CGCTATA408.3164196E-944.00000435
TACGACG207.8576064E-444.00000428
GTATCGC207.8576064E-444.00000415
TTACGAC207.8576064E-444.00000427
CGGTCAA207.8576064E-444.00000414
CGGTATG207.8576064E-444.00000417
ATTGCGA207.8576064E-444.00000433
TCGACGT207.8576064E-444.00000441
TAATGCG207.8576064E-444.00000441
ATCGCAA207.8576064E-444.00000426
ACGAAAT207.8576064E-444.00000422
Produced by FastQC (version 0.11.5)