##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527144_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1747609 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66018543049389 34.0 31.0 34.0 31.0 34.0 2 32.877828507406406 34.0 31.0 34.0 31.0 34.0 3 32.8558161465179 34.0 31.0 34.0 31.0 34.0 4 36.45488664798591 37.0 37.0 37.0 35.0 37.0 5 36.40063824345148 37.0 37.0 37.0 35.0 37.0 6 36.29792304800444 37.0 37.0 37.0 35.0 37.0 7 36.57814820134252 37.0 37.0 37.0 35.0 37.0 8 36.684705789452906 37.0 37.0 37.0 35.0 37.0 9 38.748569044906496 39.0 39.0 39.0 39.0 39.0 10 38.26376723855279 39.0 39.0 39.0 37.0 39.0 11 37.956392419585846 39.0 38.0 39.0 35.0 39.0 12 37.537092106987316 39.0 37.0 39.0 35.0 39.0 13 37.390200554014086 39.0 37.0 39.0 35.0 39.0 14 38.1555874340313 40.0 38.0 40.0 35.0 40.0 15 38.1622594069955 40.0 38.0 40.0 34.0 40.0 16 38.101756743070105 40.0 38.0 40.0 34.0 40.0 17 38.169346804691436 40.0 38.0 40.0 34.0 40.0 18 38.14390690366095 40.0 38.0 40.0 34.0 40.0 19 38.105391423367585 40.0 38.0 40.0 34.0 40.0 20 37.87825537634562 40.0 38.0 40.0 34.0 40.0 21 38.09982438863613 40.0 38.0 40.0 34.0 40.0 22 37.94112184132721 40.0 38.0 40.0 34.0 40.0 23 37.956808416527956 40.0 38.0 40.0 34.0 40.0 24 37.99221450564743 40.0 38.0 40.0 34.0 40.0 25 37.961142337902814 40.0 38.0 40.0 34.0 40.0 26 37.840393932510075 40.0 37.0 40.0 34.0 40.0 27 37.70410372114129 40.0 37.0 40.0 34.0 40.0 28 37.53795557244212 40.0 37.0 40.0 33.0 40.0 29 37.74625273731138 40.0 37.0 40.0 34.0 40.0 30 37.734002857618606 40.0 37.0 40.0 34.0 40.0 31 37.683169976808315 40.0 37.0 40.0 34.0 40.0 32 36.99188376805109 40.0 36.0 40.0 32.0 40.0 33 37.18430381166497 40.0 36.0 40.0 33.0 40.0 34 37.173799745824155 40.0 35.0 40.0 33.0 40.0 35 37.11456967777117 40.0 35.0 40.0 32.0 40.0 36 36.944276437120664 40.0 35.0 40.0 32.0 40.0 37 36.82510218246759 40.0 35.0 40.0 32.0 40.0 38 36.58528767018252 39.0 35.0 40.0 31.0 40.0 39 36.500033474306896 39.0 35.0 40.0 31.0 40.0 40 36.34583937253699 39.0 35.0 40.0 31.0 40.0 41 36.21034625021959 39.0 35.0 40.0 30.0 40.0 42 36.07313649677931 39.0 35.0 40.0 30.0 40.0 43 35.935359110647745 38.0 35.0 40.0 30.0 40.0 44 35.78865524267728 38.0 35.0 40.0 30.0 40.0 45 35.65400098076858 38.0 35.0 40.0 29.0 40.0 46 35.36084101191972 37.0 35.0 40.0 29.0 40.0 47 35.231407597466024 37.0 35.0 40.0 28.0 40.0 48 35.065070619343345 37.0 34.0 40.0 28.0 40.0 49 34.74784004888966 36.0 34.0 40.0 27.0 40.0 50 34.23424289987062 35.0 33.0 39.0 26.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 1.0 14 8.0 15 9.0 16 52.0 17 116.0 18 215.0 19 448.0 20 751.0 21 1276.0 22 1990.0 23 3085.0 24 4224.0 25 6094.0 26 8297.0 27 11370.0 28 15652.0 29 20645.0 30 26724.0 31 35197.0 32 43814.0 33 58635.0 34 102502.0 35 129259.0 36 153555.0 37 280867.0 38 525331.0 39 317489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.67309621316896 0.28518965054540235 0.03215822303501527 0.009555913250618416 8 99.27901492839646 0.647971027844329 0.04016916827505466 0.0328448754841615 9 98.05482805364359 1.2370043871369398 0.326903786831036 0.38126377238844616 10 62.63832470535458 26.336497465966357 3.958551369328036 7.066626459351033 11 34.36312127026125 25.458555088695466 19.949084720895808 20.22923892014747 12 29.863716655155702 17.21712350989266 29.111431676078574 23.807728158873065 13 24.52625272586717 16.983776119257797 31.212874275653192 27.27709687922184 14 25.601149913968168 16.98108673049864 31.227236756047834 26.190526599485352 15 24.27247742487021 18.457961706537333 30.672707682324823 26.596853186267637 16 30.102385602271447 17.77325477266368 27.452536579978705 24.67182304508617 17 31.305972903549932 18.491264350320925 23.807957043022782 26.39480570310636 18 29.212712912327643 19.71934225561896 24.314363224268128 26.753581607785264 19 30.16567206966776 18.99337895375911 23.57460965238792 27.266339324185218 20 29.262037446591314 20.470425592910086 24.123988832742334 26.143548127756265 21 31.93895201958791 18.596894385414586 25.21107410181568 24.25307949318183 22 31.864793555080112 18.41178432933225 24.761488410737183 24.961933704850455 23 29.44531642947593 21.04217819889918 24.281346685671682 25.231158685953208 24 28.972785102388464 20.463501847381195 24.176861071326595 26.386851978903746 25 28.755116276009108 19.66915940579386 23.843720191415816 27.732004126781217 26 29.28326645147742 19.888544863296083 22.97218657033696 27.856002114889545 27 28.662761521598938 18.35393386049168 23.75468425717652 29.228620360732865 28 29.877964693475484 19.462362576525987 22.601737574022565 28.057935155975965 29 30.08716480631537 20.534856481054973 22.31019638832256 27.067782324307093 30 29.930894153097178 20.324511947466508 23.34120504071563 26.40338885872069 31 30.79870840674316 21.486900101796223 22.225452031890427 25.488939459570194 32 32.744567005548724 21.54400669715022 21.948731094884497 23.76269520241656 33 33.2250520568388 19.868860826420555 22.415082549929647 24.491004566810997 34 29.351817254317186 23.32873085455614 24.924911693633987 22.394540197492688 35 29.59489222131495 23.860657618494756 25.22469270872375 21.319757451466547 36 31.521581772581854 22.532843444958225 21.680021103118605 24.265553679341316 37 29.996297798878352 25.23756744214524 21.098598141804032 23.667536617172377 38 31.979693398237245 23.930352842083096 21.251836080038498 22.838117679641158 39 31.442044530555748 22.583598505157617 20.39735432811344 25.577002636173198 40 30.034979220180258 23.878110034910556 21.813231678252972 24.273679066656214 41 28.514902360882783 22.40598440497846 23.324439276748976 25.754673957389784 42 27.01519619090998 21.498802077581427 22.691460160711006 28.794541570797588 43 26.600000343326226 21.43683169404598 25.11780381080665 26.845364151821144 44 26.887249951219065 20.620058605786536 24.479674801400083 28.013016641594312 45 27.023951009636594 21.080916841238516 23.85882654529703 28.03630560382786 46 26.763080299998453 22.687454688090984 23.634005089239068 26.91545992267149 47 27.56629200238726 21.651639468553892 24.924396704297127 25.85767182476172 48 28.336429945142193 22.16617103711414 23.80091885541903 25.696480162324637 49 27.35451694286308 22.46183213750902 25.601436019155315 24.582214900472586 50 26.28940455216241 22.969211076390657 24.839309021640425 25.90207534980651 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 29.0 4 58.0 5 32.0 6 6.0 7 20.0 8 34.0 9 549.0 10 1064.0 11 1492.0 12 1920.0 13 2767.5 14 3615.0 15 3341.5 16 3068.0 17 4409.0 18 5750.0 19 5696.0 20 5642.0 21 4834.5 22 4027.0 23 5653.5 24 7280.0 25 7706.5 26 8133.0 27 9042.5 28 9952.0 29 12320.5 30 14689.0 31 18651.0 32 22613.0 33 24161.5 34 25710.0 35 24452.5 36 23195.0 37 28490.5 38 33786.0 39 35148.5 40 36511.0 41 37595.5 42 38680.0 43 45080.0 44 51480.0 45 52160.5 46 52841.0 47 57955.0 48 63069.0 49 69195.5 50 75322.0 51 81765.0 52 88208.0 53 98059.5 54 107911.0 55 139820.0 56 171729.0 57 166464.5 58 161200.0 59 162035.5 60 162871.0 61 153437.5 62 144004.0 63 133870.5 64 123737.0 65 111104.0 66 98471.0 67 80831.5 68 63192.0 69 56468.0 70 49744.0 71 41149.0 72 32554.0 73 33147.0 74 33740.0 75 24382.5 76 15025.0 77 9920.5 78 4816.0 79 3017.0 80 1218.0 81 851.0 82 484.0 83 353.0 84 222.0 85 126.0 86 30.0 87 18.0 88 6.0 89 3.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1747609.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 8.34165715652468 #Duplication Level Percentage of deduplicated Percentage of total 1 66.31973097013953 5.5321645846585605 2 11.054649263159815 1.8442818827781475 3 4.888350457426047 1.2233083073036608 4 2.761887598163817 0.9215487779495987 5 1.5860975546175846 0.6615341008711038 6 1.0344122518183265 0.5177227417906294 7 0.7227004482517122 0.4219963556327744 8 0.5119528688599424 0.34164282498631043 9 0.3992363374053831 0.2997263385956085 >10 4.52962256236991 9.891598373261994 >50 3.1802377655558836 19.34765873968104 >100 2.828390820335528 41.823200233366265 >500 0.12468710482350692 6.936491114341623 >1k 0.053744441734270225 7.675617573975497 >5k 0.0035829627822846818 1.7945261176440412 >10k+ 7.165925564569364E-4 0.7669819331631422 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 12831 0.7342031312496101 No Hit TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 7435 0.42543841328352056 No Hit TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTC 6180 0.35362601131031024 No Hit TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 6058 0.3466450447439902 No Hit TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 5334 0.3052170136455008 No Hit TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 5014 0.28690628166826787 No Hit TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTG 4085 0.23374793789686363 No Hit TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 4032 0.23071522291313448 No Hit TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 3146 0.18001738375117088 No Hit TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 3111 0.17801464744116102 No Hit TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 3087 0.17664134254286856 No Hit TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGA 2852 0.16319439874708816 No Hit TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 2720 0.1556412218064796 No Hit TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 2684 0.15358126445904088 No Hit TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG 2536 0.14511255091957068 No Hit TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 2464 0.1409926362246933 No Hit TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT 2428 0.13893267887725458 No Hit TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC 2325 0.13303891202208273 No Hit TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG 2293 0.13120783882435946 No Hit TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT 2212 0.12657293479262238 No Hit TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAG 2148 0.12291078839717579 No Hit TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 2111 0.12079361001230825 No Hit TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA 2103 0.12033584171287744 No Hit TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 2057 0.11770367399115018 No Hit TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATG 2036 0.1165020322051443 No Hit TGATCAGGGGGGGGACTGTTCTGAGGCATCACGTGCAACGATGCTCTGAA 2002 0.11455651693256327 No Hit TGATCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA 1990 0.11386986448341704 No Hit TGATCAGGGGGTTATCTATCTTTCTAGCTTGTCATGGACTTCACACCCAA 1923 0.11003605497568392 No Hit TGATCAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT 1894 0.10837664489024719 No Hit TGATCAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC 1855 0.10614502443052193 No Hit TGATCAGGGAGAGCAAGTGGGGTTAACCAGAGCAAGCAGAGCACCTAAAA 1789 0.10236843596021766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.025921129955270315 0.0 0.0 0.0 0.0 7 0.026321677217272285 0.0 0.0 0.0 0.0 8 0.026493340329558843 0.0 0.0 0.0 0.0 9 0.02706555070384737 0.0 0.0 0.0 0.0 10 0.03570592735560414 0.0 0.0 0.0 0.0 11 0.0523572492474003 0.0 0.0 0.0 0.0 12 0.059509878926006905 0.0 0.0 0.0 0.0 13 0.0665480665297558 0.0 0.0 0.0 0.0 14 0.08474435643213099 0.0 0.0 0.0 0.0 15 0.09796241607819597 0.0 0.0 0.0 0.0 16 0.11335487514655739 0.0 0.0 0.0 0.0 17 0.12319689358432007 0.0 0.0 0.0 0.0 18 0.13326779617179815 0.0 0.0 0.0 0.0 19 0.1444831195078533 0.0 0.0 0.0 0.0 20 0.15278016993503696 0.0 0.0 0.0 0.0 21 0.16279385148508618 0.0 0.0 0.0 0.0 22 0.1722353226608469 0.0 0.0 0.0 0.0 23 0.18087569931260367 0.0 0.0 0.0 0.0 24 0.19254879094808966 0.0 0.0 0.0 0.0 25 0.20113194656241756 0.0 0.0 0.0 0.0 26 0.21063063877560714 0.0 0.0 0.0 0.0 27 0.22018655202622556 0.0 0.0 0.0 0.0 28 0.2343201482711522 0.0 0.0 0.0 0.0 29 0.2427888618106224 0.0 0.0 0.0 0.0 30 0.25400418514667755 0.0 0.0 0.0 0.0 31 0.26361731943472483 0.0 0.0 0.0 0.0 32 0.2803830834013787 0.0 0.0 0.0 5.7221037428852796E-5 33 0.29182729088714926 0.0 0.0 0.0 5.7221037428852796E-5 34 0.30384370874720834 0.0 0.0 0.0 5.7221037428852796E-5 35 0.32610269230703204 0.0 0.0 0.0 5.7221037428852796E-5 36 0.3411518251508204 0.0 0.0 0.0 5.7221037428852796E-5 37 0.35282491678630634 0.0 0.0 0.0 5.7221037428852796E-5 38 0.36524188190836737 0.0 0.0 0.0 5.7221037428852796E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTTC 20 7.8576064E-4 44.000004 39 TCGCAAA 20 7.8576064E-4 44.000004 32 ATATGTC 20 7.8576064E-4 44.000004 10 CGTCTAA 20 7.8576064E-4 44.000004 24 CCGTCTA 20 7.8576064E-4 44.000004 35 CATTACG 20 7.8576064E-4 44.000004 21 GTTACGA 20 7.8576064E-4 44.000004 41 CGGATTA 20 7.8576064E-4 44.000004 40 AATTGCG 20 7.8576064E-4 44.000004 32 CGCTATA 40 8.3164196E-9 44.000004 35 TACGACG 20 7.8576064E-4 44.000004 28 GTATCGC 20 7.8576064E-4 44.000004 15 TTACGAC 20 7.8576064E-4 44.000004 27 CGGTCAA 20 7.8576064E-4 44.000004 14 CGGTATG 20 7.8576064E-4 44.000004 17 ATTGCGA 20 7.8576064E-4 44.000004 33 TCGACGT 20 7.8576064E-4 44.000004 41 TAATGCG 20 7.8576064E-4 44.000004 41 ATCGCAA 20 7.8576064E-4 44.000004 26 ACGAAAT 20 7.8576064E-4 44.000004 22 >>END_MODULE