FastQCFastQC Report
Fri 17 Jun 2016
SRR1527143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864261
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG123870.6644455899683575No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT66400.3561733040598929No Hit
TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCAC59490.3191076785922143No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG59050.3167474940472391No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA56660.3039274007233966No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT48210.25860112934830476No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT37340.2002938429758494No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC34080.18280702111989686No Hit
TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG30700.16467651256986013No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC30560.1639255447600953No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC29850.16011706515343077No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG26290.14102102656226784No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG25300.13571061133607365No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC25000.1341013946008633No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23710.12718176263945874No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT22780.12219319076030663No Hit
TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA22600.12122766071918042No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT22240.11929660063692799No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG22220.1191893195212473No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA21300.11425438819993552No Hit
TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT21240.11393254485289345No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT21210.11377162317937242No Hit
TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG19960.10706655344932925No Hit
TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAGAG19920.10685199121796787No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19320.10363355774754715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG351.0200347E-746.00000441
AACGTTC206.312471E-446.039
CGACGTA253.41798E-546.025
CAACGAA206.312471E-446.011
TCCGATA405.6152203E-946.025
CCGATAG301.8620303E-646.012
ACCGATA253.41798E-546.011
CGTCTAT501.6370905E-1146.033
CGTTAAT2450.046.041
CGTAGTC253.41798E-546.038
GCGATAA253.41798E-546.09
TTACGCA206.312471E-446.014
TCATACG253.41798E-546.018
CGGTCAA206.312471E-446.014
TATACGA253.41798E-546.017
CAATACG253.41798E-546.012
CGATAAT206.312471E-446.010
ACGACGT301.8620303E-646.013
AATCGTC253.41798E-546.025
CGAAGTA206.312471E-446.010
Produced by FastQC (version 0.11.5)