##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1864261
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60094536119138	34.0	31.0	34.0	31.0	34.0
2	32.812095516668535	34.0	31.0	34.0	31.0	34.0
3	32.77953355243713	34.0	31.0	34.0	31.0	34.0
4	36.381569962574986	37.0	37.0	37.0	35.0	37.0
5	36.33295767062659	37.0	37.0	37.0	35.0	37.0
6	36.31036373125866	37.0	37.0	37.0	35.0	37.0
7	36.57317510799185	37.0	37.0	37.0	35.0	37.0
8	36.644952611249174	37.0	37.0	37.0	35.0	37.0
9	38.7129001786767	39.0	39.0	39.0	39.0	39.0
10	38.16557391910253	39.0	39.0	39.0	37.0	39.0
11	37.856441775051884	39.0	38.0	39.0	35.0	39.0
12	37.44955561479857	39.0	37.0	39.0	35.0	39.0
13	37.35405396561962	39.0	37.0	39.0	35.0	39.0
14	38.13659031648466	40.0	38.0	40.0	35.0	40.0
15	38.05876215830294	40.0	38.0	40.0	34.0	40.0
16	33.03568759953676	40.0	35.0	40.0	3.0	40.0
17	34.76047774426435	40.0	35.0	40.0	17.0	40.0
18	36.829502950498885	40.0	35.0	40.0	31.0	40.0
19	37.693749963122116	40.0	37.0	40.0	34.0	40.0
20	37.9373794763716	40.0	38.0	40.0	34.0	40.0
21	38.132672946545576	40.0	38.0	40.0	34.0	40.0
22	38.170107082645615	40.0	38.0	40.0	34.0	40.0
23	38.093524458216955	40.0	38.0	40.0	34.0	40.0
24	38.05941228186396	40.0	38.0	40.0	34.0	40.0
25	38.02608003922197	40.0	38.0	40.0	34.0	40.0
26	37.93475377106532	40.0	38.0	40.0	34.0	40.0
27	37.808934478595006	40.0	37.0	40.0	34.0	40.0
28	37.69213913717017	40.0	37.0	40.0	34.0	40.0
29	37.505502716626054	40.0	37.0	40.0	33.0	40.0
30	37.453539499029375	40.0	36.0	40.0	33.0	40.0
31	37.41694376484838	40.0	36.0	40.0	33.0	40.0
32	37.370309200267556	40.0	36.0	40.0	33.0	40.0
33	37.01970324970591	39.0	35.0	40.0	32.0	40.0
34	36.940089933759275	39.0	35.0	40.0	32.0	40.0
35	36.79743394299403	39.0	35.0	40.0	31.0	40.0
36	36.778495607642924	39.0	35.0	40.0	32.0	40.0
37	36.41689548834632	39.0	35.0	40.0	31.0	40.0
38	36.38234131379673	39.0	35.0	40.0	31.0	40.0
39	35.95162211728937	38.0	35.0	40.0	30.0	40.0
40	35.87559038138973	38.0	35.0	40.0	30.0	40.0
41	35.82518005794253	38.0	35.0	40.0	30.0	40.0
42	35.73057688810741	38.0	35.0	40.0	30.0	40.0
43	35.59370334947735	38.0	34.0	40.0	30.0	40.0
44	35.840089987399836	38.0	35.0	40.0	30.0	40.0
45	35.89046008042865	38.0	35.0	40.0	31.0	40.0
46	35.828274045318764	37.0	35.0	40.0	31.0	40.0
47	35.47867546443336	37.0	35.0	40.0	30.0	40.0
48	35.386229181428995	37.0	35.0	40.0	29.0	40.0
49	35.27596833276027	36.0	34.0	40.0	29.0	40.0
50	34.75478058061613	36.0	34.0	40.0	27.0	40.0
51	34.830304876838596	36.0	34.0	40.0	28.0	40.0
52	34.46928246634994	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	12.0
16	37.0
17	97.0
18	215.0
19	397.0
20	738.0
21	1280.0
22	2124.0
23	3157.0
24	4806.0
25	6773.0
26	9575.0
27	13223.0
28	18131.0
29	23431.0
30	30342.0
31	40147.0
32	53864.0
33	80828.0
34	120324.0
35	157136.0
36	221378.0
37	371720.0
38	465346.0
39	239176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.68561269049773	0.2838121915332671	0.023226361544869522	0.0073487564241273085
8	99.24844214409893	0.6685222723642237	0.047740096477907334	0.03529548705894722
9	98.10579098098388	1.2242920921480416	0.2993679533069672	0.37054897356110544
10	62.96258946574541	26.090016365734197	3.9006340850342305	7.046760083486165
11	34.44303131374845	25.499165621122792	19.78848455232395	20.26931851280481
12	29.893721962750924	17.170664408041578	29.08943543849279	23.846178190714713
13	24.711454029237323	16.789119120123203	31.21826825750257	27.281158593136904
14	25.793008596972207	16.856545301328516	31.150734795181577	26.199711306517703
15	24.326851229522045	18.49338692382665	30.53746229739291	26.642299549258393
16	28.547236679842573	25.975064650282338	24.155845131127027	21.321853538748062
17	31.412822560789504	18.38567668368324	23.706229975309252	26.495270780218007
18	29.34921666011358	19.59258923509101	24.31344109006196	26.74475301473345
19	30.26566559081588	18.903254426284732	23.509476409150864	27.321603573748526
20	29.256686697838987	20.37735059629526	24.13803646592403	26.227926239941723
21	32.107360503706296	18.479976784366567	25.144118768777545	24.26854394314959
22	31.750114388489592	18.386374010935164	24.81363929192318	25.04987230865206
23	29.479187731760735	20.918476543788664	24.274444404512032	25.327891319938573
24	29.086967972832127	20.411626912755242	24.142166788877738	26.35923832553489
25	28.794090527023847	19.633302418491834	23.77896657174076	27.793640482743566
26	29.424206159974382	19.76005505666857	22.863107687174704	27.952631096182344
27	28.819194308093127	18.323078152683557	23.753326385093075	29.104401154130244
28	29.943661322100283	19.360808384662878	22.572590425911393	28.122939867325442
29	30.264378217427712	20.527919642153112	22.143734165977833	27.06396797444135
30	30.027394232889066	20.27613086365053	23.175134812131994	26.521340091328415
31	30.7819559600292	21.43701981643128	22.26297712605692	25.518047097482594
32	32.916689240401425	21.176004861980164	21.995418023549277	23.911887874069134
33	33.57324966836725	19.713065928000425	22.424757048503402	24.288927355128923
34	29.45054367387399	23.241005417159936	24.87425312228277	22.434197786683303
35	29.63302885164685	23.885496719611684	25.128241163656806	21.353233265084665
36	31.554755476835055	22.526727748957896	21.618914948067893	24.29960182613915
37	30.237397016834016	25.239545321175523	20.957205026549396	23.56585263544107
38	31.961029061917834	23.834109065200636	21.213553252468405	22.99130862041313
39	31.668312537783066	22.570981209176182	20.36308220790973	25.397624045131018
40	30.220446600556468	23.777947401141795	21.745989429591674	24.255616568710067
41	28.479005890269654	22.38071815051648	23.366577963064184	25.77369799614968
42	27.032266404757703	21.474943690824407	22.610085175841796	28.882704728576098
43	26.617839454883196	21.390191609436663	25.20033407339423	26.791634862285914
44	26.916295518706878	20.537038536985968	24.42812460272462	28.118541341582535
45	26.993698843670494	20.951519127418315	23.94476953602527	28.11001249288592
46	26.467377690141024	22.677189513700068	23.729778180201162	27.125654615957746
47	27.65310222120186	21.672019100329837	24.93175580028762	25.743122878180685
48	28.52481492666531	22.14764992670018	23.691747024692358	25.635788121942156
49	27.443099437256908	22.248386894324344	25.75047163460481	24.558042033813933
50	26.243589282831103	23.033309177202117	24.665376790052466	26.057724749914307
51	26.178845129517807	23.506579819027486	23.274584406367993	27.039990645086714
52	27.195816465612914	22.791765745247044	24.275517215668835	25.736900573471207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	28.0
4	56.0
5	32.0
6	8.0
7	23.0
8	38.0
9	526.0
10	1014.0
11	1418.0
12	1822.0
13	2727.0
14	3602.5
15	3573.0
16	4468.0
17	5363.0
18	5662.0
19	5961.0
20	5025.0
21	4089.0
22	5045.0
23	6001.0
24	6541.5
25	7082.0
26	8028.5
27	8975.0
28	12270.5
29	15566.0
30	15638.0
31	15710.0
32	19532.0
33	23354.0
34	25373.5
35	27393.0
36	27231.5
37	27070.0
38	31195.5
39	34579.5
40	33838.0
41	38716.0
42	43594.0
43	48581.0
44	53568.0
45	53465.0
46	53362.0
47	60224.5
48	67087.0
49	68521.0
50	69955.0
51	83469.0
52	96983.0
53	105198.0
54	113413.0
55	137990.5
56	162568.0
57	174732.0
58	186896.0
59	173329.0
60	159762.0
61	155681.5
62	151601.0
63	139635.5
64	120008.5
65	112347.0
66	94165.5
67	75984.0
68	63912.0
69	51840.0
70	48230.0
71	44620.0
72	40795.0
73	36970.0
74	28468.0
75	19966.0
76	13630.0
77	7294.0
78	4676.5
79	2059.0
80	1319.5
81	580.0
82	404.0
83	228.0
84	129.0
85	30.0
86	17.5
87	5.0
88	5.5
89	4.0
90	2.0
91	3.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1864261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.826772730302487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.27136251285157	6.905407088624902
2	10.194347801087444	2.0035507794989043
3	4.341276275684561	1.2798220596181855
4	2.28646523628287	0.8987429693075656
5	1.3633740351011931	0.6698783394667435
6	0.8235922422848443	0.48559522724240334
7	0.5891736184221128	0.4052772672846842
8	0.42783100293031184	0.3363358426218842
9	0.32272301418965366	0.285419314375192
>10	4.0996306962399975	10.60933253262741
>50	2.8260439945990377	20.11738482223293
>100	2.305308170744269	39.748424104241955
>500	0.10364464527869331	6.772357018107103
>1k	0.04208600747680273	7.22654289004104
>5k	0.002512597461301656	1.491319742582761
>10k+	6.28149365325414E-4	0.7646100021263518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	12387	0.6644455899683575	No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6640	0.3561733040598929	No Hit
TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCAC	5949	0.3191076785922143	No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5905	0.3167474940472391	No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5666	0.3039274007233966	No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4821	0.25860112934830476	No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	3734	0.2002938429758494	No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3408	0.18280702111989686	No Hit
TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3070	0.16467651256986013	No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3056	0.1639255447600953	No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2985	0.16011706515343077	No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2629	0.14102102656226784	No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG	2530	0.13571061133607365	No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2500	0.1341013946008633	No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2371	0.12718176263945874	No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2278	0.12219319076030663	No Hit
TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	2260	0.12122766071918042	No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2224	0.11929660063692799	No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2222	0.1191893195212473	No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2130	0.11425438819993552	No Hit
TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT	2124	0.11393254485289345	No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2121	0.11377162317937242	No Hit
TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1996	0.10706655344932925	No Hit
TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAGAG	1992	0.10685199121796787	No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1932	0.10363355774754715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.02429917270167643	0.0	0.0	0.0	0.0
7	0.02499649995360092	0.0	0.0	0.0	0.0
8	0.025050140511441263	0.0	0.0	0.0	0.0
9	0.02553290553200437	0.0	0.0	0.0	0.0
10	0.03261345916692995	0.0	0.0	0.0	0.0
11	0.04779373703574768	0.0	0.0	0.0	0.0
12	0.054337885092269805	0.0	0.0	0.0	0.0
13	0.060184705896867446	0.0	0.0	0.0	0.0
14	0.07879797946746726	0.0	0.0	0.0	0.0
15	0.0922081189275536	0.0	0.0	0.0	0.0
16	0.11033862747759031	0.0	0.0	0.0	0.0
17	0.1201548495623735	0.0	0.0	0.0	0.0
18	0.1306683988990812	0.0	0.0	0.0	0.0
19	0.14161107269851164	0.0	0.0	0.0	0.0
20	0.1500862808372862	0.0	0.0	0.0	0.0
21	0.1588296917652625	0.0	0.0	0.0	0.0
22	0.1673585404618774	0.0	0.0	0.0	5.3640557840345316E-5
23	0.1765847164104168	0.0	0.0	0.0	5.3640557840345316E-5
24	0.186561860168721	0.0	0.0	0.0	5.3640557840345316E-5
25	0.1941251788242097	0.0	0.0	0.0	5.3640557840345316E-5
26	0.202868589752186	0.0	0.0	0.0	5.3640557840345316E-5
27	0.2135430607624147	0.0	0.0	0.0	5.3640557840345316E-5
28	0.22727504356954312	0.0	0.0	0.0	5.3640557840345316E-5
29	0.2360184544975194	0.0	0.0	0.0	5.3640557840345316E-5
30	0.24658564439206743	0.0	0.0	0.0	5.3640557840345316E-5
31	0.25763559930717855	0.0	0.0	0.0	5.3640557840345316E-5
32	0.27351320442792076	0.0	0.0	0.0	5.3640557840345316E-5
33	0.2831148642813426	0.0	0.0	0.0	5.3640557840345316E-5
34	0.29566675481598337	0.0	0.0	0.0	5.3640557840345316E-5
35	0.3172838996256426	0.0	0.0	0.0	5.3640557840345316E-5
36	0.33031855518084646	0.0	0.0	0.0	5.3640557840345316E-5
37	0.34211947790572245	0.0	0.0	0.0	5.3640557840345316E-5
38	0.3530085111473125	0.0	0.0	0.0	5.3640557840345316E-5
39	0.3657749639133147	0.0	0.0	0.0	5.3640557840345316E-5
40	0.3788096194685186	0.0	0.0	0.0	5.3640557840345316E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	35	1.0200347E-7	46.000004	41
AACGTTC	20	6.312471E-4	46.0	39
CGACGTA	25	3.41798E-5	46.0	25
CAACGAA	20	6.312471E-4	46.0	11
TCCGATA	40	5.6152203E-9	46.0	25
CCGATAG	30	1.8620303E-6	46.0	12
ACCGATA	25	3.41798E-5	46.0	11
CGTCTAT	50	1.6370905E-11	46.0	33
CGTTAAT	245	0.0	46.0	41
CGTAGTC	25	3.41798E-5	46.0	38
GCGATAA	25	3.41798E-5	46.0	9
TTACGCA	20	6.312471E-4	46.0	14
TCATACG	25	3.41798E-5	46.0	18
CGGTCAA	20	6.312471E-4	46.0	14
TATACGA	25	3.41798E-5	46.0	17
CAATACG	25	3.41798E-5	46.0	12
CGATAAT	20	6.312471E-4	46.0	10
ACGACGT	30	1.8620303E-6	46.0	13
AATCGTC	25	3.41798E-5	46.0	25
CGAAGTA	20	6.312471E-4	46.0	10
>>END_MODULE