FastQCFastQC Report
Fri 17 Jun 2016
SRR1527142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810163
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG118190.6529246261248296No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT72350.39968776292521724No Hit
TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCAC57470.31748522094419124No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG57020.3149992569729908No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA53880.2976527528183926No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT49530.2736217677634556No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT37740.2084895117180055No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC32020.1768901474618584No Hit
TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG29280.16175338905943829No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC28890.1595988869510646No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28780.15899120686921564No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG25260.1395454442500482No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG24550.13562314553993204No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC23650.1306512175975313No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT23020.12717086803785074No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG22850.12623172609317504No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT21020.11612213927696013No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG20660.11413336809999984No Hit
TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA20600.11380190623717311No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT20340.11236557149825735No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19970.11032155667749259No Hit
TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAGAG19780.10927192744520797No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA19680.10871949100716345No Hit
TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG19190.10601255246074524No Hit
TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT18890.10435524314661167No Hit
TGATCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT18540.1024217156134558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG950.046.045
CGTATAG501.6370905E-1146.021
ATAATCC206.3124206E-446.010
ACCGATA301.8620012E-646.011
CGACAAT206.3124206E-446.028
CCCCGTA253.4179397E-546.034
TCGTACC206.3124206E-446.014
TTTATCG501.6370905E-1146.045
CGACTAA253.4179397E-546.037
TCGGTTA206.3124206E-446.035
TAATGCG301.8620012E-646.041
TACGTTT453.110472E-1045.99999646
TACCGTA6550.045.29770726
CGTTAAT2950.045.2203441
ATTCGTA1050.043.80952546
TAGCGTA1000.043.742
CAGGGAT145350.043.4207085
TGATCAG1912450.043.404691
GATCAGG1900300.043.3820462
TCAGGGG1179400.043.341954
Produced by FastQC (version 0.11.5)