##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1810163
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58718910948903	34.0	31.0	34.0	31.0	34.0
2	32.808752029513364	34.0	31.0	34.0	31.0	34.0
3	32.77437225266454	34.0	31.0	34.0	31.0	34.0
4	36.39298726136818	37.0	37.0	37.0	35.0	37.0
5	36.3577064606889	37.0	37.0	37.0	35.0	37.0
6	36.30332075067273	37.0	37.0	37.0	35.0	37.0
7	36.57418144111884	37.0	37.0	37.0	35.0	37.0
8	36.66693496663008	37.0	37.0	37.0	35.0	37.0
9	38.66110841951802	39.0	39.0	39.0	39.0	39.0
10	38.15870062530281	39.0	39.0	39.0	37.0	39.0
11	37.781203681657395	39.0	38.0	39.0	35.0	39.0
12	37.41214520460312	39.0	37.0	39.0	35.0	39.0
13	37.25979041666413	39.0	37.0	39.0	35.0	39.0
14	38.063772157534984	40.0	38.0	40.0	34.0	40.0
15	38.171399481704135	40.0	38.0	40.0	34.0	40.0
16	38.285128466331486	40.0	38.0	40.0	35.0	40.0
17	38.25623769793107	40.0	38.0	40.0	35.0	40.0
18	38.25304848237424	40.0	38.0	40.0	35.0	40.0
19	38.21334874262705	40.0	38.0	40.0	34.0	40.0
20	38.16902897694848	40.0	38.0	40.0	34.0	40.0
21	33.89055626482256	40.0	34.0	40.0	10.0	40.0
22	34.68280812280441	40.0	34.0	40.0	17.0	40.0
23	36.69714440080811	40.0	34.0	40.0	30.0	40.0
24	37.451489727720656	40.0	37.0	40.0	33.0	40.0
25	37.74267897421392	40.0	37.0	40.0	34.0	40.0
26	37.8180821285155	40.0	37.0	40.0	34.0	40.0
27	37.76904124103741	40.0	37.0	40.0	34.0	40.0
28	37.66059465363064	40.0	37.0	40.0	33.0	40.0
29	37.66851438240645	40.0	37.0	40.0	34.0	40.0
30	37.5274254307485	40.0	37.0	40.0	33.0	40.0
31	37.38774298226182	40.0	36.0	40.0	33.0	40.0
32	37.36770224559888	40.0	36.0	40.0	33.0	40.0
33	37.040459892285945	39.0	35.0	40.0	32.0	40.0
34	36.947752218999064	39.0	35.0	40.0	32.0	40.0
35	36.80668867941727	39.0	35.0	40.0	31.0	40.0
36	36.719568900701205	39.0	35.0	40.0	31.0	40.0
37	36.5756387684424	39.0	35.0	40.0	31.0	40.0
38	36.46759324988965	39.0	35.0	40.0	31.0	40.0
39	36.255349932575136	38.0	35.0	40.0	31.0	40.0
40	31.328380924811743	37.0	30.0	40.0	8.0	40.0
41	32.22622327381567	36.0	30.0	40.0	13.0	40.0
42	33.99301333636805	36.0	31.0	40.0	23.0	40.0
43	34.83978459398408	37.0	33.0	40.0	27.0	40.0
44	35.55174699736985	37.0	34.0	40.0	30.0	40.0
45	35.77370711919313	38.0	35.0	40.0	30.0	40.0
46	35.80526836533505	37.0	35.0	40.0	31.0	40.0
47	35.700705958524175	37.0	35.0	40.0	30.0	40.0
48	35.50631241495932	37.0	35.0	40.0	30.0	40.0
49	35.33906891257859	36.0	35.0	40.0	30.0	40.0
50	35.19886275434864	36.0	34.0	40.0	29.0	40.0
51	35.032806990309716	36.0	34.0	40.0	29.0	40.0
52	34.61643288477336	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	2.0
15	17.0
16	48.0
17	96.0
18	242.0
19	442.0
20	788.0
21	1313.0
22	2134.0
23	3352.0
24	4953.0
25	7258.0
26	10206.0
27	13824.0
28	18820.0
29	24614.0
30	32650.0
31	44578.0
32	62636.0
33	78677.0
34	135765.0
35	181505.0
36	234524.0
37	261229.0
38	447527.0
39	242959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	99.6849454993832	0.28809560244022225	0.02099258464569213	0.005966313530880921
8	99.26851891238525	0.6657963951312672	0.031157415105711475	0.034527277377783105
9	98.0211726789245	1.2525391359783622	0.34123998778010595	0.38504819731703716
10	62.85964302662247	26.12980157035582	3.9382088795318433	7.072346523489874
11	34.50716869143828	25.4385378554307	19.812359439453793	20.24193401367722
12	29.884159603306443	17.260821263057526	29.02108815614947	23.83393097748656
13	24.74826852609406	16.88312047036648	31.23735265829652	27.13125834524294
14	25.75022249377542	16.873508076344507	31.132776440574688	26.243492989305384
15	24.26908515973423	18.43027395875399	30.63519694082798	26.665443940683797
16	29.997077611242744	17.67299408948255	27.511776563768013	24.818151735506692
17	31.397835443548455	18.431047369767253	23.70874888062567	26.462368306058625
18	29.31769127973558	19.620940213671364	24.291569322762648	26.769799183830408
19	30.171758012952427	19.006465163634434	23.559259580490817	27.262517242922325
20	29.246592710159252	20.448324266930655	24.100813020705868	26.204270002204222
21	36.20646317486326	18.12234588818797	23.848073350300496	21.823117586648273
22	31.81265996487609	18.420053884650166	24.71108955381366	25.05619659666008
23	29.43027782580906	20.996396457114635	24.23527604972591	25.338049667350397
24	29.219026131900826	20.454124849530125	24.06683817976613	26.260010838802916
25	28.80917353851559	19.59320790448153	23.861221337525958	27.73639721947692
26	29.36492459518839	19.800095350529208	22.82772324923225	28.00725680505015
27	28.73293731006545	18.37033460522616	23.743828594441496	29.152899490266897
28	29.916035185781613	19.40609768291585	22.528634161674944	28.149232969627597
29	30.172365693034276	20.504009859885546	22.21993268009566	27.10369176698452
30	30.106018076825126	20.31027040106333	23.152003438364392	26.431708083747154
31	30.789381950686206	21.489225003494163	22.23120238343177	25.490190662387864
32	32.8722330530455	21.21455360649842	21.96128193980321	23.95193140065287
33	33.4814047132772	19.76601002230186	22.3730128170778	24.37957244734314
34	29.366250442639696	23.279174306402243	24.864114447151998	22.490460803806066
35	29.703623375353487	23.920387280040526	25.150939445784715	21.225049898821265
36	31.6465423279561	22.476152700060712	21.652083265429688	24.225221706553498
37	30.106404782331758	25.307113226819904	20.984850535559506	23.60163145528883
38	31.993859116554695	23.897019218711243	21.18151790750336	22.927603757230703
39	31.454183960229	22.56990116359687	20.35010106824634	25.625813807927795
40	30.21473756783229	28.156911836116414	20.0357094913552	21.5926411046961
41	28.511355054765787	22.39511027459958	23.330606138784187	25.76292853185045
42	27.077782498040232	21.55557261970331	22.609787074423686	28.756857807832777
43	26.602410943102917	21.462210861673782	25.13320623612349	26.80217195909982
44	26.840234829681087	20.641179827452003	24.40426635612373	28.114318986743182
45	26.940115337679536	21.06395943348748	23.944528752383075	28.051396476449913
46	26.445905700204897	22.747398991140578	23.69996514126076	27.106730167393767
47	27.505534032018108	21.66699904925689	24.923390876954173	25.904076041770825
48	28.39777412310383	22.22391022244958	23.667537122347547	25.71077853209904
49	27.429518778143187	22.34655111169547	25.71635814012329	24.507571970038057
50	26.15123610415195	23.02013686060316	24.79754585636763	26.031081178877262
51	26.189906654815065	23.507606773533656	23.258126478112747	27.04436009353854
52	27.20959383215766	22.835788821227702	24.328803538686845	25.625813807927795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	20.5
4	41.0
5	23.5
6	6.0
7	19.0
8	32.0
9	476.5
10	921.0
11	1275.0
12	1629.0
13	2593.0
14	3336.5
15	3116.0
16	4129.0
17	5142.0
18	5472.0
19	5802.0
20	4872.0
21	3942.0
22	5014.0
23	6086.0
24	6460.5
25	6835.0
26	7683.5
27	8532.0
28	11505.5
29	14479.0
30	14906.5
31	15334.0
32	18738.0
33	22142.0
34	24553.0
35	26964.0
36	26697.5
37	26431.0
38	30378.0
39	33470.5
40	32616.0
41	37491.0
42	42366.0
43	47199.5
44	52033.0
45	51819.5
46	51606.0
47	58678.0
48	65750.0
49	66742.5
50	67735.0
51	80598.0
52	93461.0
53	101742.5
54	110024.0
55	133070.0
56	156116.0
57	168717.0
58	181318.0
59	168431.5
60	155545.0
61	152060.0
62	148575.0
63	135732.5
64	116237.5
65	109585.0
66	92522.0
67	75459.0
68	62994.0
69	50529.0
70	46747.5
71	42966.0
72	39643.5
73	36321.0
74	28001.0
75	19681.0
76	13426.5
77	7172.0
78	4704.0
79	2236.0
80	1410.0
81	584.0
82	406.0
83	228.0
84	129.0
85	30.0
86	19.0
87	8.0
88	6.0
89	4.0
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1810163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.009938975272252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67613947330935	7.074638431356569
2	9.72846124228928	1.9476260671723393
3	4.30074374276416	1.2915054724005968
4	2.272085866866904	0.9097376349566508
5	1.3429179908394753	0.6721263568549184
6	0.8629234071839003	0.5182686387746911
7	0.5850694291791603	0.40995564976665355
8	0.3922072027993063	0.31407761325466277
9	0.3154213860376451	0.2841613943159356
>10	4.316426823880422	11.418882162979152
>50	2.850989976875779	20.650092985981374
>100	2.2240539509635178	39.21109994083029
>500	0.08901338876588798	6.037580423880709
>1k	0.040344197785978	6.959560306844137
>5k	0.002561536367363683	1.5430647113047071
>10k+	6.403840918409207E-4	0.7576222093266174
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11819	0.6529246261248296	No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7235	0.39968776292521724	No Hit
TGATCAGGGGGTTCTTCACTTTACCTACCGTATGCTAAGGCCCGCCCCTCAC	5747	0.31748522094419124	No Hit
TGATCAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5702	0.3149992569729908	No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5388	0.2976527528183926	No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4953	0.2736217677634556	No Hit
TGATCAGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAGGAGCGCCGACTGCT	3774	0.2084895117180055	No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3202	0.1768901474618584	No Hit
TGATCAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2928	0.16175338905943829	No Hit
TGATCAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2889	0.1595988869510646	No Hit
TGATCAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2878	0.15899120686921564	No Hit
TGATCAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2526	0.1395454442500482	No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGATCAGGGAAG	2455	0.13562314553993204	No Hit
TGATCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2365	0.1306512175975313	No Hit
TGATCAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2302	0.12717086803785074	No Hit
TGATCAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2285	0.12623172609317504	No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2102	0.11612213927696013	No Hit
TGATCAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2066	0.11413336809999984	No Hit
TGATCAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA	2060	0.11380190623717311	No Hit
TGATCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2034	0.11236557149825735	No Hit
TGATCAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1997	0.11032155667749259	No Hit
TGATCAGGGGGAGCTGCCTTCCAAAACACTTACTTCACAGCGTTAATCAGAG	1978	0.10927192744520797	No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1968	0.10871949100716345	No Hit
TGATCAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1919	0.10601255246074524	No Hit
TGATCAGGGATGTTGATTTCAGAAGAGACTAGCTTTGTGGAATATAGATGCT	1889	0.10435524314661167	No Hit
TGATCAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	1854	0.1024217156134558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.030439247736253585	0.0	0.0	0.0	0.0
7	0.03110217146190702	0.0	0.0	0.0	0.0
8	0.03126790239332038	0.0	0.0	0.0	0.0
9	0.031709851543756	0.0	0.0	0.0	0.0
10	0.04065932184007739	0.0	0.0	0.0	0.0
11	0.054746451010212895	0.0	0.0	0.0	0.0
12	0.06253580478664077	0.0	0.0	0.0	0.0
13	0.06839163102991277	0.0	0.0	0.0	0.0
14	0.08286546570667945	0.0	0.0	0.0	0.0
15	0.0958477220007259	0.0	0.0	0.0	0.0
16	0.11153691684119055	0.0	0.0	0.0	0.0
17	0.12125979815077427	0.0	0.0	0.0	0.0
18	0.12960158836524666	0.0	0.0	0.0	0.0
19	0.13949020060624376	0.0	0.0	0.0	0.0
20	0.1476662598893028	0.0	0.0	0.0	0.0
21	0.15617378103518853	0.0	0.0	0.0	0.0
22	0.16606239327618563	0.0	0.0	0.0	0.0
23	0.17567478729816044	0.0	0.0	0.0	0.0
24	0.18777314529133565	0.0	0.0	0.0	0.0
25	0.19523103720493679	0.0	0.0	0.0	0.0
26	0.2053406240211517	0.0	0.0	0.0	0.0
27	0.216720814644869	0.0	0.0	0.0	0.0
28	0.2313051366092446	0.0	0.0	0.0	0.0
29	0.23959168317991253	1.1048728760890594E-4	0.0	0.0	0.0
30	0.2493698081333007	1.1048728760890594E-4	0.0	0.0	0.0
31	0.2580430602105998	1.1048728760890594E-4	0.0	0.0	0.0
32	0.2721301893807353	1.1048728760890594E-4	0.0	0.0	0.0
33	0.28218453255314574	1.1048728760890594E-4	0.0	0.0	0.0
34	0.29268082487599184	1.1048728760890594E-4	0.0	0.0	0.0
35	0.3120161002075504	1.1048728760890594E-4	0.0	0.0	0.0
36	0.3241144582007256	1.1048728760890594E-4	0.0	0.0	0.0
37	0.3354946488244429	1.1048728760890594E-4	0.0	0.0	0.0
38	0.3465986212291379	1.1048728760890594E-4	0.0	0.0	0.0
39	0.3585864919347042	1.1048728760890594E-4	0.0	0.0	0.0
40	0.3739994685561466	1.1048728760890594E-4	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTG	95	0.0	46.0	45
CGTATAG	50	1.6370905E-11	46.0	21
ATAATCC	20	6.3124206E-4	46.0	10
ACCGATA	30	1.8620012E-6	46.0	11
CGACAAT	20	6.3124206E-4	46.0	28
CCCCGTA	25	3.4179397E-5	46.0	34
TCGTACC	20	6.3124206E-4	46.0	14
TTTATCG	50	1.6370905E-11	46.0	45
CGACTAA	25	3.4179397E-5	46.0	37
TCGGTTA	20	6.3124206E-4	46.0	35
TAATGCG	30	1.8620012E-6	46.0	41
TACGTTT	45	3.110472E-10	45.999996	46
TACCGTA	655	0.0	45.297707	26
CGTTAAT	295	0.0	45.22034	41
ATTCGTA	105	0.0	43.809525	46
TAGCGTA	100	0.0	43.7	42
CAGGGAT	14535	0.0	43.420708	5
TGATCAG	191245	0.0	43.40469	1
GATCAGG	190030	0.0	43.382046	2
TCAGGGG	117940	0.0	43.34195	4
>>END_MODULE