FastQCFastQC Report
Fri 17 Jun 2016
SRR1527141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86050
Sequences flagged as poor quality0
Sequence length50
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGA48865.678094131319No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG9241.073794305636258Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC7140.8297501452643812Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGG6970.8099941894247531No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG6550.7611853573503776No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAA5190.6031377106333528No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.5671121441022662No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC4080.47414294015107494No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAG3700.4299825682742592No Hit
GCGTCCGGGGCTACTTCCGACTGGCGCAGGTCGAGCTACCGGCAGCCGCT3540.4113887274840209No Hit
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA3520.4090644973852411No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGA3510.40790238233585124No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGC3490.4055781522370715No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAA3360.3904706565950029No Hit
GCGTCCGGGGCGCAGTGGCTCACGCCTGTAATCCCAACACTTTGGGAGGC3270.3800116211504939No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA3260.378849506101104Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT3220.37420104590354447Illumina Single End Adapter 2 (96% over 33bp)
GCGTCCGGGAGTCGTCCTGCCATCGAGTTGCAGGTTGCCTTGCAGAACCT3180.36955258570598487No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGA3130.3637420104590354No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGA2980.3463102847181871No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT2950.34282393957001744Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGGTCGCGTCGGTAGGCTTGCGCGATCTGGCGGAATATCGCGC2930.34049970947123764No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCG2890.3358512492736781No Hit
GCGTCCGGGTGTATTGCGTGAAAACGCCATCCGAGGACATGGCCTTGGTG2870.3335270191748983No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC2860.3323649041255084No Hit
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2860.3323649041255084Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCG2790.3242300987797792No Hit
GCGTCCGGGAATCCAGCAACGCCTGGGTCGCCGACTCCGACAACATCGGC2700.3137710633352702No Hit
GCGTCCCTCTGGGGCTGTGGTCACCCTGGAGTGTGTTGAGAAACTGATCC2550.2963393375944218No Hit
GCGTCCGCCGCCAGCCCCCTGCAACTGCATGACGAAGCACTTGATGCACT2550.2963393375944218No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCG2510.2916908773968623No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATG2390.27774549680418364No Hit
GCGTCCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCT2360.27425915165601394Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA2320.2696106914584544No Hit
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA2290.2661243463102847Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGCAGAGAGCAGTGCTGCGTCCGGGGCCCTCGGTGGCGGCGCT2160.25101685066821616No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTAT2100.24404416037187684No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC2070.24055781522370712Illumina Single End Adapter 2 (100% over 21bp)
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTT2030.23590935502614757Illumina Single End Adapter 2 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAG2030.23590935502614757No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGT1990.23126089482858803No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1990.23126089482858803Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGTACCACATGGTACATGATCCGGGACTCGGGCCTGTCTGTCC1910.2219639744334689No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATT1820.21150493898895992No Hit
GCGTCCGGGGGGGTGGAACTGGCACTGGCGCACCCCGAAGTGGGCAGCGA1680.19523532829750145No Hit
GCGTCCGGGAAGCAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA1670.19407321324811155Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAGCTACTCCAGAGGCTGAAGCAGGAGAATGGCGTGAACCTG1660.19291109819872168No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGG1620.1882626380011621No Hit
GCGTCCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTC1550.1801278326554329Illumina Single End Adapter 2 (96% over 25bp)
GCGTCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.178965717606043No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGT1510.17547937245787334No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCGGCCTGGA1490.17315514235909354No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTT1490.17315514235909354No Hit
GCGTCCGGGGTTAGCGGGGGCGCAGAGTGCCGTTCTGCGGCGGAGACGGC1450.16850668216153397No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGAAG1440.1673445671121441No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAGAGCTCGT1440.1673445671121441No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAGGGGCTCGTATGCCG1400.16269610691458455No Hit
GCGTCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT1370.15920976176641488No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAAGACGGCATACGAGCTCTTC1330.15456130156885534Illumina Single End Adapter 1 (100% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTCGTATGCCG1320.15339918651946544No Hit
GCGTCCGGGTTTCTCCTTTTTCTTCTTTCTCCCAGCCAGCAAGAAGGATA1320.15339918651946544No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTGCGGGA1310.15223707147007554No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTTCGGGA1300.15107495642068564No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAAGAGCTCG1280.14875072632190586No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAA1280.14875072632190586No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAACGCC1190.13829169087739687No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTTCTGCTTGA1180.13712957582800697No Hit
GCGTCCGGGGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.12899477048227775No Hit
GCGTCCGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG1100.12783265543288785No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGG1060.12318419523532831No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCGGGAGCGGGC1050.12202208018593842No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACGTCCGGGA1050.12202208018593842No Hit
GCGTCCGGGAAGCAGTGGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCT1040.12085996513654851Illumina Single End Adapter 2 (96% over 32bp)
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGA1020.11853573503776874Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAACTGGTTCAACAGTTGATAAAAAGTTATCCGCAACTTTTC1010.11737361998837885No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGGATGCCGT990.11504938988959906No Hit
GCGTCCGGGCTGCGTCCGGGGCTCAACGCGCGCGGCGTGATCTCGGTCGC990.11504938988959906No Hit
GCGTCCGGGATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTG980.11388727484020918No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGA980.11388727484020918No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTACGGGA970.11272515979081929No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGAAGAGCTCGT960.1115630447414294No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAA950.11040092969203952No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCG950.11040092969203952No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGCCCGGGA940.10923881464264963No Hit
GCGTCCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA940.10923881464264963Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.10923881464264963No Hit
GCGTCCAGGAAAGGAAGTTCTCGGTGAGGTTCTCTCTGTGAAAGGTTAGA930.10807669959325975No Hit
GCGTCCGGGAAGCAGTGGTATCAGATCGGAAGAGCTCGTATGCCGTCTTC930.10807669959325975Illumina Single End Adapter 2 (100% over 28bp)
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAA930.10807669959325975No Hit
GCGTCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.10691458454386985No Hit
GCGTCCGGGCAGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAA910.10575246949447996No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCTGGA900.10459035444509006No Hit
GCGTCCGGGAAGCAGTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG900.10459035444509006Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCCGGAAGAGCTC890.10342823939570017No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGGGAAAAAAAAAAAAAAAAA880.10226612434631027No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCCGGGAGAGCTC870.10110400929692039No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAG207.815899E-444.033
AGGGGTA351.4253055E-744.014
TGAGAAA502.7284841E-1144.037
TTATGAA351.4253055E-744.07
ATCCCAA351.4253055E-744.031
CGACAAC254.40849E-544.038
TAGCGGG302.5004156E-644.012
GTTGATG207.815899E-444.036
GGTGATC302.5004156E-644.017
GAGGTAT207.815899E-444.015
TTTTGAC351.4253055E-744.016
TGTTGAG502.7284841E-1144.034
GTATGGG207.815899E-444.043
CATGATC302.5004156E-644.022
AACGCGA351.4253055E-744.023
TGGTCAC454.6929927E-1044.018
AACGCCT254.40849E-544.018
AACGCCA207.815899E-444.023
GGGTTTC207.815899E-444.07
GTATGAA408.1599865E-944.018
Produced by FastQC (version 0.11.5)