FastQCFastQC Report
Fri 17 Jun 2016
SRR1527140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96087
Sequences flagged as poor quality0
Sequence length52
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG54165.636558535493876No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC8590.8939814959359746Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA6360.6619001529863561No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT6360.6619001529863561Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG5870.6109047009480991No Hit
GCGTCCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG5220.5432576727340847No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA4970.5172395849594639No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA4610.4797735385640097No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.4235744689708285No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGAAG3880.4038007222621166No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG3830.3985971047071924No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT3530.36737539937764735No Hit
GCGTCCGGGGCTACTTCCGACTGGCGCAGGTCGAGCTACCGGCAGCCGCTTC3330.3465609291579506No Hit
GCGTCCGGGGTCGCGTCGGTAGGCTTGCGCGATCTGGCGGAATATCGCGCCT3240.3371944175590871No Hit
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA3210.33407224702613253No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG3180.33095007649317804No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCGTA3130.3257464589382539No Hit
GCGTCCGGGAGTCGTCCTGCCATCGAGTTGCAGGTTGCCTTGCAGAACCTTG3080.32054284138332967No Hit
GCGTCCGGGGCGCAGTGGCTCACGCCTGTAATCCCAACACTTTGGGAGGCCG3060.31846139436136No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA3020.3142985003174207No Hit
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2940.305972712229542Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA2880.29972837116363293Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC2750.2861989655208301No Hit
GCGTCCGGGAATCCAGCAACGCCTGGGTCGCCGACTCCGACAACATCGGCTC2640.2747510068999969No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCGC2610.2716288363670423No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT2590.2695473893450727Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGTGTATTGCGTGAAAACGCCATCCGAGGACATGGCCTTGGTGGC2520.26226232476817884No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2500.2601808777462092No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA2470.25705870721325463No Hit
GCGTCCGCCGCCAGCCCCCTGCAACTGCATGACGAAGCACTTGATGCACTGC2460.2560179837022698No Hit
GCGTCCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC2330.24248857805946694Illumina Single End Adapter 2 (96% over 28bp)
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2270.23624423699355793Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2250.23416278997158824Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG2190.22791844890567922No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC2110.21959266081780054Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCCTCTGGGGCTGTGGTCACCCTGGAGTGTGTTGAGAAACTGATCCGG2040.21230759624090667No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT2020.210226149218937Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC2020.210226149218937Illumina Single End Adapter 2 (95% over 21bp)
GCGTCCGGGAAGCAGGGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG1980.20606325517499766No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT1970.20502253166401282No Hit
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA1810.18837095548825544No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGAAGCA1750.1821266144223464No Hit
GCGTCCGGGCAGAGAGCAGTGCTGCGTCCGGGGCCCTCGGTGGCGGCGCTGC1730.18004516740037674No Hit
GCGTCCGGGAGCTACTCCAGAGGCTGAAGCAGGAGAATGGCGTGAACCTGGG1700.17692299686742224No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG1690.17588227335643739Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT1620.16859720877954354No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAGAGCTCGTAT1580.16443431473560421No Hit
GCGTCCGGGTACCACATGGTACATGATCCGGGACTCGGGCCTGTCTGTCCGC1550.1613121442026497No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA1500.1561085266477255No Hit
GCGTCCGGGGGGGTGGAACTGGCACTGGCGCACCCCGAAGTGGGCAGCGAGT1470.152986356114771No Hit
GCGTCCGGGAAGCAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA1450.1509049090928013Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGTTTCTCCTTTTTCTTCTTTCTCCCAGCCAGCAAGAAGGATAGA1360.1415383974939378No Hit
GCGTCCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTCTT1310.1363347799390136Illumina Single End Adapter 2 (96% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCAGCGTCCGGGAAG1240.12904971536211973No Hit
GCGTCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC1230.1280089918511349No Hit
GCGTCCGGGAAGCAGTGGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1220.12696826834015007Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGTAG1210.12592754482916524No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGAAG1210.12592754482916524No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGGAG1200.12488682131818041No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAGCT1190.12384609780719558No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1170.12176465078522589Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAAGACGGCATACGAGCTCTTCCG1160.12072392727424106Illumina Single End Adapter 1 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAGGGGCTCGTATGCCGTC1160.12072392727424106No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCAG1120.1165610332303017No Hit
GCGTCCGGGCTGCGTCCGGGGCTCAACGCGCGCGGCGTGATCTCGGTCGCGG1120.1165610332303017No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAACGCCGG1090.1134388626973472No Hit
GCGTCCGGGATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCT1070.11135741567537753No Hit
GCGTCCGGGAAGCAGAGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1070.11135741567537753Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAA1060.11031669216439267No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCGGATGCCGTCT1050.10927596865340786No Hit
GCGTCCGGGAAGCAGAGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG1030.10719452163143817No Hit
GCGTCCAGGAAAGGAAGTTCTCGGTGAGGTTCTCTCTGTGAAAGGTTAGAAA1010.1051130746094685No Hit
GCGTCCGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA970.10095018056552915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC351.0065378E-746.00000438
CCGGATC551.8189894E-1246.0000045
ACCGGCA551.8189894E-1246.00000438
TGCGTTG750.046.00000440
GAGTGAA351.0065378E-746.00000429
GTCGTCC351.0065378E-746.00000411
AGCTACT351.0065378E-746.00000410
CTCCAGA351.0065378E-746.00000415
ACTCCGA351.0065378E-746.00000434
ACTCCAG351.0065378E-746.00000414
GCTACTC351.0065378E-746.00000411
TGCCTCG551.8189894E-1246.00000437
TCCGGAT551.8189894E-1246.0000044
AGTGCCT700.046.00000435
AGCGGGC351.0065378E-746.00000444
GACAACA351.0065378E-746.00000439
CGCAGGT551.8189894E-1246.00000425
TCGTCCT351.0065378E-746.00000412
GCTTGGT351.0065378E-746.00000438
TGGCGGA351.0065378E-746.00000435
Produced by FastQC (version 0.11.5)