FastQCFastQC Report
Fri 17 Jun 2016
SRR1527139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93948
Sequences flagged as poor quality0
Sequence length52
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGAAG52445.581811214714523No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC8480.9026269851407162Illumina Single End Adapter 2 (100% over 25bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT6530.6950653553029336Illumina Single End Adapter 2 (95% over 24bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA5800.6173627964405842No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGGAG5110.5439179120364457No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAA4670.49708349299612553No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGGAAG3790.40341465491548517No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.39489930599906325No Hit
GCGTCCGGGGCTACTTCCGACTGGCGCAGGTCGAGCTACCGGCAGCCGCTTC3550.3778686081662196No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGAAGCA3540.3768041895516669No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT3350.35658023587516496No Hit
GCGTCCGGGGTCGCGTCGGTAGGCTTGCGCGATCTGGCGGAATATCGCGCCT3280.34912930557329586No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGACCGGGAAG3260.3470004683441904No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAA3070.32677651466768853No Hit
GCGTCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2990.3182611657512666No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGCGGAAGGGCTCGTATGCCGTC2820.3001660493038702No Hit
GCGTCCGGGAAGCAGTGGTAGCAACGCAGAGTGCAGTGCAGCGTCCGGGAAG2730.29058628177289564No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT2700.2873930259292375Illumina Single End Adapter 2 (96% over 26bp)
GCGTCCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA2700.2873930259292375Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAGTCGTCCTGCCATCGAGTTGCAGGTTGCCTTGCAGAACCTTG2700.2873930259292375No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGAAGAGCTCGTA2660.28313535147102653No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA2640.2810065142419211No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGAAG2570.2735555839400519No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2560.2724911653254992No Hit
GCGTCCGGGGCGCAGTGGCTCACGCCTGTAATCCCAACACTTTGGGAGGCCG2520.26823349086728826No Hit
GCGTCCGGGTGTATTGCGTGAAAACGCCATCCGAGGACATGGCCTTGGTGGC2480.26397581640907736No Hit
GCGTCCGGGAATCCAGCAACGCCTGGGTCGCCGACTCCGACAACATCGGCTC2420.25758930472176095No Hit
GCGTCCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA2390.2543960488781028Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCCTCTGGGGCTGTGGTCACCCTGGAGTGTGTTGAGAAACTGATCCGG2320.24694511857623366No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCAGCGTCCGGGAAG2270.24162302550347No Hit
GCGTCCGCCGCCAGCCCCCTGCAACTGCATGACGAAGCACTTGATGCACTGC2270.24162302550347No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG2260.24055860688891728No Hit
GCGTCCGGGCAGAGAGCAGTGCTGCGTCCGGGGCCCTCGGTGGCGGCGCTGC2220.23630093243070635No Hit
GCGTCCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT2210.2352365138161536Illumina Single End Adapter 2 (100% over 29bp)
GCGTCCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC2200.23417209520160087Illumina Single End Adapter 2 (96% over 28bp)
GCGTCCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA2170.2309788393579427Illumina Single End Adapter 2 (100% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCGC2110.22459232767062628No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG1990.2118193042959935Illumina Single End Adapter 2 (100% over 23bp)
GCGTCCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1960.20862604845233532Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC1900.20223953676501896Illumina Single End Adapter 2 (95% over 22bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC1860.197981862306808Illumina Single End Adapter 2 (95% over 21bp)
GCGTCCTTATGAATGTTTTGACCATTTTCATGGTGGAAGAATTTTATATTTA1830.19478860646314983No Hit
GCGTCCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGAGTCCGGAAGCA1640.17456465278664793No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGGAGAGCTCGTAT1630.1735002341720952No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT1630.1735002341720952No Hit
GCGTCCGGGTACCACATGGTACATGATCCGGGACTCGGGCCTGTCTGTCCGC1560.1660493038702261No Hit
GCGTCCGGGAGCTACTCCAGAGGCTGAAGCAGGAGAATGGCGTGAACCTGGG1480.15753395495380423No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGGAAAAAAAAAAAAAAAAAAAAA1440.1532762804955933No Hit
GCGTCCGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC1360.14476093157917144No Hit
GCGTCCGGGGGGGTGGAACTGGCACTGGCGCACCCCGAAGTGGGCAGCGAGT1360.14476093157917144No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT1350.14369651296461874No Hit
GCGTCCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1340.142632094350066Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGGCCGGGGAG1330.14156767573551327No Hit
GCGTCCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTCTT1320.14050325712096054Illumina Single End Adapter 2 (96% over 27bp)
GCGTCCGGGAAGCAGTGGTAGCAACGCAGAGTGCAGTGCGGCGTCCGGGAAG1310.1394388385064078No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAAGACGGCATACGAGCTCTTCCG1250.1330523268190914Illumina Single End Adapter 1 (100% over 27bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGATCGGAAGAGCT1240.13198790820453868No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTCCGGGAAG1210.12879465236088047No Hit
GCGTCCGGGTTTCTCCTTTTTCTTCTTTCTCCCAGCCAGCAAGAAGGATAGA1210.12879465236088047No Hit
GCGTCCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAACGCCGG1150.12240814067356409No Hit
GCGTCCGGGAACTGGTTCAACAGTTGATAAAAAGTTATCCGCAACTTTTCTC1130.12027930344445864No Hit
GCGTCCGGGAAGCAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA1130.12027930344445864Illumina Single End Adapter 2 (97% over 34bp)
GCGTCCGGGAAGCAGTGGTATCAGATCGGAAGAGCTCGTATGCCGTCTTCTG1110.11815046621535318Illumina Single End Adapter 2 (100% over 30bp)
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCGGCCTGGAAA1090.11602162898624771No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTCGGATGCCGTCT1080.11495721037169497No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGTGG1010.10750628006982586No Hit
GCGTCCGGGCTGCGTCCGGGGCTCAACGCGCGCGGCGTGATCTCGGTCGCGG990.1053774428407204No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGTAG980.10431302422616766No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGTAAAAAAAAAAAAAAAAAAAAA960.1021841869970622No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCGTCCGGGCAG940.10005534976795674No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAA351.0062104E-746.00000437
TGTTGAG351.0062104E-746.00000434
TGGTCAC351.0062104E-746.00000418
GTTGAGA351.0062104E-746.00000435
AAACTGA351.0062104E-746.00000441
GAGAAAC351.0062104E-746.00000438
TTGCAGG351.0062104E-746.00000428
CAGTTCG351.0062104E-746.00000434
GTCACCC351.0062104E-746.00000420
CAACATT351.0062104E-746.00000424
AACCTTG351.0062104E-746.00000446
CTGATCC351.0062104E-746.00000444
GGTCACC351.0062104E-746.00000419
TTGAGAA351.0062104E-746.00000436
GCAACAT351.0062104E-746.00000423
ACATTAA351.0062104E-746.00000426
CGGGTGT351.0062104E-746.0000046
CGGGTAC351.0062104E-746.0000046
CATTAAC351.0062104E-746.00000427
TTGGGAA351.0062104E-746.00000445
Produced by FastQC (version 0.11.5)