FastQCFastQC Report
Fri 17 Jun 2016
SRR1527138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671144
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC120750.7225589177234277No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG75800.4535814986619945No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT41660.24929030652056317No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC41200.246537701119712No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGA40960.24510155917144183No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT40440.24198991828352315No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC32960.1972301608957696No Hit
GCCTAGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA31050.18580086455745284No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC31020.1856213468139191No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTG30440.18215067043893285No Hit
GCCTAGGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT30330.18149243871264237No Hit
GCCTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC28250.16904587516096756No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG26820.16048886271919116No Hit
GCCTAGGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG23500.1406222324347872No Hit
GCCTAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA23420.14014351845203046No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA22660.1355957356158416No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT21700.12985116782276093No Hit
GCCTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21130.1264403306956193No Hit
GCCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG20950.12536322423441665No Hit
GCCTAGGGGAAATTAAAGGAGCATCCCATCTTCTTCATGTTCATTCAGAT20910.1251238672430383No Hit
GCCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA19670.1177038005103091No Hit
GCCTAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA18010.10777048536810713No Hit
GCCTAGGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT18010.10777048536810713No Hit
GCCTAGGGTGATAGTCTTTCTGAAGTGGGCTTTCATCAGGCTCAGAGCCC17900.10711225364181663No Hit
GCCTAGGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG17880.10699257514612745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA302.5284098E-644.00000411
CGCATTC302.5284098E-644.00000434
TACGACA302.5284098E-644.00000437
CGAATAG302.5284098E-644.00000410
ACCGTCA302.5284098E-644.0000049
TATACGC302.5284098E-644.00000419
AACGTAT207.8575086E-444.036
GATACGG254.4437107E-544.09
ACACGTG254.4437107E-544.040
TTAATCG254.4437107E-544.032
AATCACG254.4437107E-544.025
CATGCGA254.4437107E-544.023
ACGTTAC254.4437107E-544.031
TAAACGA408.3164196E-944.034
CGCGATT207.8575086E-444.033
ATAGACG254.4437107E-544.010
TAACGCT207.8575086E-444.018
TAACGCG207.8575086E-444.010
ATCACGT254.4437107E-544.040
TCGATAC502.7284841E-1144.025
Produced by FastQC (version 0.11.5)