##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1671144
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33728511726099	33.0	31.0	34.0	31.0	34.0
2	32.580003279190784	34.0	31.0	34.0	31.0	34.0
3	32.96556550482783	34.0	31.0	34.0	31.0	34.0
4	36.4117490772788	37.0	37.0	37.0	35.0	37.0
5	35.842843584993275	37.0	35.0	37.0	35.0	37.0
6	36.18519888172413	37.0	37.0	37.0	35.0	37.0
7	36.60220842728095	37.0	37.0	37.0	35.0	37.0
8	36.654188388313635	37.0	37.0	37.0	35.0	37.0
9	38.6460245197302	39.0	39.0	39.0	38.0	39.0
10	38.09007063424816	39.0	39.0	39.0	37.0	39.0
11	37.009319364459316	39.0	35.0	39.0	34.0	39.0
12	36.748057019622486	39.0	35.0	39.0	33.0	39.0
13	36.68417922094086	39.0	35.0	39.0	33.0	39.0
14	37.46544163758479	40.0	35.0	40.0	33.0	40.0
15	37.51281098457105	40.0	35.0	40.0	33.0	40.0
16	37.343795029034005	40.0	35.0	40.0	33.0	40.0
17	37.37151077345818	40.0	35.0	40.0	33.0	40.0
18	37.35478510529314	40.0	35.0	40.0	33.0	40.0
19	37.21188539108539	40.0	35.0	40.0	33.0	40.0
20	37.04358810491496	40.0	35.0	40.0	32.0	40.0
21	37.224992579933264	40.0	35.0	40.0	33.0	40.0
22	37.02078755630873	39.0	35.0	40.0	32.0	40.0
23	37.079774693264014	39.0	35.0	40.0	33.0	40.0
24	37.13849195521152	39.0	35.0	40.0	33.0	40.0
25	37.05391037516815	39.0	35.0	40.0	33.0	40.0
26	36.90129037354052	39.0	35.0	40.0	32.0	40.0
27	36.815051246331855	39.0	35.0	40.0	32.0	40.0
28	36.574685365234835	39.0	35.0	40.0	31.0	40.0
29	36.85532126495383	39.0	35.0	40.0	32.0	40.0
30	36.88569447037479	39.0	35.0	40.0	32.0	40.0
31	36.83725639442203	39.0	35.0	40.0	32.0	40.0
32	36.09364064377456	39.0	35.0	40.0	30.0	40.0
33	36.30753483841009	39.0	35.0	40.0	31.0	40.0
34	36.16129310221022	39.0	35.0	40.0	30.0	40.0
35	36.219261176774715	39.0	35.0	40.0	31.0	40.0
36	36.05725239716027	38.0	35.0	40.0	30.0	40.0
37	35.962804521932284	38.0	35.0	40.0	30.0	40.0
38	35.78410657609398	38.0	35.0	40.0	30.0	40.0
39	35.729174146572646	38.0	35.0	40.0	30.0	40.0
40	35.572050044759756	38.0	35.0	40.0	30.0	40.0
41	35.441603476420944	37.0	35.0	40.0	29.0	40.0
42	35.299729406921244	37.0	35.0	40.0	29.0	40.0
43	35.160703685618955	37.0	34.0	40.0	28.0	40.0
44	35.03810922338231	36.0	34.0	40.0	28.0	40.0
45	34.97535400899025	36.0	34.0	40.0	28.0	40.0
46	34.646214808538346	36.0	34.0	40.0	26.0	40.0
47	34.55635839879747	35.0	34.0	40.0	26.0	40.0
48	34.40730601312634	35.0	34.0	40.0	26.0	40.0
49	34.105076522430146	35.0	33.0	39.0	25.0	40.0
50	33.69736120884855	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	5.0
15	28.0
16	81.0
17	177.0
18	424.0
19	801.0
20	1362.0
21	2116.0
22	3226.0
23	4939.0
24	6717.0
25	9335.0
26	12793.0
27	16557.0
28	21544.0
29	27871.0
30	35495.0
31	43329.0
32	54147.0
33	78327.0
34	166238.0
35	153252.0
36	135324.0
37	229223.0
38	445284.0
39	222544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.25159052720771	0.6429128788422781	0.08251832277769001	0.02297827117232267
8	99.12808231965647	0.6792353022839444	0.1287740613615583	0.06390831669802242
9	95.4586199633305	2.295433547318484	1.378277395604448	0.8676690937465592
10	56.18217221256816	29.420026042040664	6.105637814574926	8.292163930816256
11	33.74640366120454	22.00247255772094	21.776639236355454	22.47448454471907
12	30.568341208178353	14.710820850866233	29.806467904621027	24.91437003633439
13	25.869165074942675	14.670848233306048	29.16122129511281	30.298765396638473
14	22.903113077029865	17.887327483448466	32.94982359389736	26.259735845624316
15	26.293904056143575	16.88490040355589	30.321264953828038	26.499930586472498
16	29.886413139741396	18.065050049546898	28.836593375555907	23.211943435155796
17	31.694456013365695	20.451319575093468	22.831545336607736	25.0226790749331
18	32.67151125217217	18.370469570545687	24.258352362214147	24.699666815068003
19	29.889405102133626	20.79982335454036	23.87849281689669	25.43227872642932
20	30.781907483735694	22.039931926871652	23.544470135428185	23.63369045396447
21	31.737181236326734	18.66475899144538	27.200887535724032	22.397172236503856
22	29.533780452193227	19.131385446137497	25.83601413163677	25.498819970032503
23	29.125018550166832	20.606901619489403	25.124944349499508	25.143135480844258
24	29.77134226613625	20.385795598703645	24.306044242746285	25.536817892413822
25	28.9173763601461	20.13794143413135	23.022612054975514	27.922070150747036
26	27.603785191461654	19.80930428496886	24.690990124130536	27.895920399438946
27	25.842476770403987	18.466212367097032	24.931543900465787	30.75976696203319
28	26.78063649811147	23.071859755951614	23.116978548826435	27.03052519711048
29	31.523973996256455	21.400011010421604	21.532435265901682	25.54357972742026
30	28.964709205191173	21.14371951190322	23.547581776316104	26.343989506589498
31	31.164280277462623	20.712517891935107	23.254010426390543	24.869191404211726
32	32.43574461566448	20.42798226843408	23.488221242454273	23.64805187344717
33	33.69099251770045	19.348302719574136	23.152223865806896	23.808480896918518
34	28.12474568319666	20.910466123805012	25.832483616013942	25.13230457698439
35	26.7444337531655	25.337672875587025	26.360205942755382	21.5576874284921
36	29.523847137051025	24.813720421459788	23.032904405604786	22.629528035884398
37	29.93865280310973	24.959249472217834	22.620671827203402	22.48142589746904
38	30.410545111612166	23.799923884476744	23.090290244287743	22.699240759623347
39	30.437412933894386	21.874177210342136	21.99283843881796	25.69557141694552
40	28.119659347129872	22.34732614304931	22.662200265207545	26.87081424461327
41	25.74069020982034	24.22759498882203	22.903472112516933	27.128242688840697
42	24.794871058388747	23.102916325582953	23.640272771227373	28.461939844800927
43	26.45912021944249	20.90502075225115	24.418482189446273	28.217376838860087
44	25.640339791184964	21.217261947504223	25.094186976107384	28.04821128520343
45	26.68423546983384	21.461585596453688	24.78972488307411	27.064454050638364
46	27.890115992398023	22.816944560133656	23.209968739976926	26.08297070749139
47	27.307580914630936	21.490248596171245	25.096161671286254	26.106008817911565
48	27.7985619431958	20.729512238322968	25.742126351768608	25.72979946671262
49	27.838115686021077	22.688529534259168	24.448102617129344	25.025252162590416
50	24.333689975250486	22.737238682004662	25.444964646972373	27.484106695772475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	4.0
10	7.0
11	65.0
12	123.0
13	499.5
14	876.0
15	1969.0
16	3062.0
17	3850.5
18	4639.0
19	4760.0
20	4881.0
21	5028.5
22	5176.0
23	5649.0
24	6122.0
25	6408.0
26	6694.0
27	6394.5
28	6095.0
29	7799.0
30	9503.0
31	11044.5
32	12586.0
33	11511.0
34	10436.0
35	12315.5
36	14195.0
37	16988.5
38	19782.0
39	21602.5
40	23423.0
41	26784.0
42	30145.0
43	35646.5
44	41148.0
45	39369.0
46	37590.0
47	42441.5
48	47293.0
49	52068.0
50	56843.0
51	60403.5
52	63964.0
53	72408.5
54	80853.0
55	87819.0
56	94785.0
57	106880.0
58	118975.0
59	136298.0
60	153621.0
61	157267.0
62	160913.0
63	163556.5
64	166200.0
65	157951.5
66	149703.0
67	132185.0
68	114667.0
69	94062.0
70	73457.0
71	62256.5
72	51056.0
73	45990.5
74	40925.0
75	32451.5
76	23978.0
77	22400.0
78	20822.0
79	16599.0
80	12376.0
81	7523.5
82	2671.0
83	1867.0
84	1063.0
85	724.0
86	385.0
87	228.0
88	71.0
89	52.0
90	33.0
91	18.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1671144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.570924130036657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.45402176428486	5.78143302807527
2	10.791318329715043	1.849831413341231
3	4.715594419370361	1.2125100598934289
4	2.533242880736055	0.8684893013497692
5	1.4795946568247853	0.634074677342643
6	0.8922036103657818	0.45882056717939423
7	0.6326254609595539	0.3795529380029662
8	0.4661432640762609	0.31962228401002196
9	0.31602479212107387	0.24377620648322978
>10	4.512530504040204	10.380673521212751
>50	3.2020021110136723	19.908529190535518
>100	2.8270518865543632	42.156859692907545
>500	0.12522609438252652	6.971292488650503
>1k	0.05096410817893521	7.608035943578488
>5k	7.28058688270503E-4	0.47300229207704125
>10k+	7.28058688270503E-4	0.7534963953601943
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	12075	0.7225589177234277	No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7580	0.4535814986619945	No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	4166	0.24929030652056317	No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4120	0.246537701119712	No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGA	4096	0.24510155917144183	No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4044	0.24198991828352315	No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3296	0.1972301608957696	No Hit
GCCTAGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	3105	0.18580086455745284	No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	3102	0.1856213468139191	No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTG	3044	0.18215067043893285	No Hit
GCCTAGGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCAT	3033	0.18149243871264237	No Hit
GCCTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2825	0.16904587516096756	No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2682	0.16048886271919116	No Hit
GCCTAGGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG	2350	0.1406222324347872	No Hit
GCCTAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	2342	0.14014351845203046	No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2266	0.1355957356158416	No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2170	0.12985116782276093	No Hit
GCCTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2113	0.1264403306956193	No Hit
GCCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2095	0.12536322423441665	No Hit
GCCTAGGGGAAATTAAAGGAGCATCCCATCTTCTTCATGTTCATTCAGAT	2091	0.1251238672430383	No Hit
GCCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1967	0.1177038005103091	No Hit
GCCTAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1801	0.10777048536810713	No Hit
GCCTAGGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT	1801	0.10777048536810713	No Hit
GCCTAGGGTGATAGTCTTTCTGAAGTGGGCTTTCATCAGGCTCAGAGCCC	1790	0.10711225364181663	No Hit
GCCTAGGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG	1788	0.10699257514612745	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.023696342146457754	0.0	0.0	0.0	0.0
6	0.023696342146457754	0.0	0.0	0.0	0.0
7	0.023756181394302345	0.0	0.0	0.0	0.0
8	0.024055377633525298	0.0	0.0	0.0	0.0
9	0.02668830453868727	0.0	0.0	0.0	0.0
10	0.04224650897828074	0.0	0.0	0.0	0.0
11	0.05696696394804996	0.0	0.0	0.0	0.0
12	0.06313040647604276	0.0	0.0	0.0	0.0
13	0.06977256298679228	0.0	0.0	0.0	0.0
14	0.10286366704485071	0.0	0.0	0.0	0.0
15	0.11273714293920811	0.0	0.0	0.0	0.0
16	0.128235508130957	0.0	0.0	0.0	0.0
17	0.14080175017832097	0.0	0.0	0.0	0.0
18	0.1515728147903472	0.0	0.0	0.0	0.0
19	0.16216436165883968	0.0	0.0	0.0	0.0
20	0.1707812133484607	0.0	0.0	0.0	0.0
21	0.18155227796048695	0.0	0.0	0.0	0.0
22	0.19088720062424303	0.0	0.0	0.0	0.0
23	0.2004614802793775	0.0	0.0	0.0	0.0
24	0.2133269185659644	0.0	0.0	0.0	0.0
25	0.22056746755515982	0.0	0.0	0.0	0.0
26	0.22852608751849032	0.0	0.0	0.0	0.0
27	0.23839956341284774	0.0	0.0	0.0	0.0
28	0.2794492874342367	0.0	0.0	0.0	0.0
29	0.2873480681497226	0.0	0.0	0.0	0.0
30	0.30476128927249835	0.0	0.0	0.0	0.0
31	0.3143355689276328	0.0	0.0	0.0	0.0
32	0.3229524206172538	0.0	0.0	0.0	0.0
33	0.32923554164093577	0.0	0.0	0.0	0.0
34	0.33880982129607023	0.0	0.0	0.0	0.0
35	0.39350289382602577	0.0	0.0	0.0	0.0
36	0.4112751504358691	0.0	0.0	0.0	0.0
37	0.42150766181729404	0.0	0.0	0.0	0.0
38	0.429107246293557	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	30	2.5284098E-6	44.000004	11
CGCATTC	30	2.5284098E-6	44.000004	34
TACGACA	30	2.5284098E-6	44.000004	37
CGAATAG	30	2.5284098E-6	44.000004	10
ACCGTCA	30	2.5284098E-6	44.000004	9
TATACGC	30	2.5284098E-6	44.000004	19
AACGTAT	20	7.8575086E-4	44.0	36
GATACGG	25	4.4437107E-5	44.0	9
ACACGTG	25	4.4437107E-5	44.0	40
TTAATCG	25	4.4437107E-5	44.0	32
AATCACG	25	4.4437107E-5	44.0	25
CATGCGA	25	4.4437107E-5	44.0	23
ACGTTAC	25	4.4437107E-5	44.0	31
TAAACGA	40	8.3164196E-9	44.0	34
CGCGATT	20	7.8575086E-4	44.0	33
ATAGACG	25	4.4437107E-5	44.0	10
TAACGCT	20	7.8575086E-4	44.0	18
TAACGCG	20	7.8575086E-4	44.0	10
ATCACGT	25	4.4437107E-5	44.0	40
TCGATAC	50	2.7284841E-11	44.0	25
>>END_MODULE