FastQCFastQC Report
Fri 17 Jun 2016
SRR1527137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797491
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC115660.6434524567855973No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT72080.40100339862619616No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGAAG42460.23621815074456565No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT40430.22492463105517635No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT39690.2208077815132315No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG38700.2153001044233323No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA32600.18136391225324633No Hit
GCCTAGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG30370.1689577305254936No Hit
GCCTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT28240.1571078798169226No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCA28120.15644028259390452No Hit
GCCTAGGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC28030.15593958467664096No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT27100.15076570619825078No Hit
GCCTAGGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT24360.13552223627267118No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC23180.1289575302463267No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21980.12228155801614583No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21360.11883230569721906No Hit
GCCTAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA21000.11682951402816483No Hit
GCCTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20860.11605065060131038No Hit
GCCTAGGGGAAATTAAAGGAGCATCCCATCTTCTTCATGTTCATTCAGATTG20840.11593938439747405No Hit
GCCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG19460.10826201633276607No Hit
GCCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA19250.1070937211924844No Hit
GCCTAGGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC18170.10108534618532164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTA351.0199983E-746.00000421
ATCCGAT351.0199983E-746.00000428
CAACGAC253.417931E-546.038
ACCGATT253.417931E-546.012
TTCGTAC253.417931E-546.022
TAACGCG453.110472E-1046.010
TAACGAT206.3124095E-446.035
TTATCGG301.861994E-646.014
CTAACGC405.6152203E-946.017
CCCGTAA253.417931E-546.039
AAACCGG206.3124095E-446.026
AACGATA206.3124095E-446.017
TCCGTAT253.417931E-546.040
ACGTCTA253.417931E-546.034
ACGTCCC206.3124095E-446.019
CGTCGAT301.861994E-646.040
TTACGAC301.861994E-646.012
CCGAACT253.417931E-546.046
CGCGTAT405.6152203E-946.039
TAATGCG206.3124095E-446.019
Produced by FastQC (version 0.11.5)