##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527137_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1797491
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.359230171388894	33.0	31.0	34.0	31.0	34.0
2	32.554843390036446	34.0	31.0	34.0	31.0	34.0
3	33.008894064003655	34.0	31.0	34.0	31.0	34.0
4	36.38760027171207	37.0	37.0	37.0	35.0	37.0
5	35.97674870138432	37.0	35.0	37.0	35.0	37.0
6	36.24771361859392	37.0	37.0	37.0	35.0	37.0
7	36.60903392562188	37.0	37.0	37.0	35.0	37.0
8	36.61960087700022	37.0	37.0	37.0	35.0	37.0
9	38.61027843811179	39.0	39.0	39.0	38.0	39.0
10	37.97766108425578	39.0	38.0	39.0	35.0	39.0
11	36.92158792450143	39.0	35.0	39.0	34.0	39.0
12	36.6872384896503	39.0	35.0	39.0	33.0	39.0
13	36.67983038579887	39.0	35.0	39.0	33.0	39.0
14	37.47230389470656	40.0	35.0	40.0	33.0	40.0
15	37.42203104215821	40.0	35.0	40.0	33.0	40.0
16	32.40126376154317	38.0	33.0	40.0	3.0	40.0
17	33.96180731920215	38.0	33.0	40.0	17.0	40.0
18	35.95908407886326	39.0	34.0	40.0	30.0	40.0
19	36.78698140908633	39.0	35.0	40.0	31.0	40.0
20	37.13633392322966	39.0	35.0	40.0	33.0	40.0
21	37.35094083920309	40.0	35.0	40.0	33.0	40.0
22	37.37331480380152	40.0	35.0	40.0	33.0	40.0
23	37.33515049588566	40.0	35.0	40.0	33.0	40.0
24	37.31334009460965	40.0	35.0	40.0	33.0	40.0
25	37.24886188581751	40.0	35.0	40.0	33.0	40.0
26	37.10983309513094	39.0	35.0	40.0	33.0	40.0
27	37.0249252986524	39.0	35.0	40.0	33.0	40.0
28	36.88361666344922	39.0	35.0	40.0	32.0	40.0
29	36.702386270640574	39.0	35.0	40.0	32.0	40.0
30	36.64148860828789	39.0	35.0	40.0	32.0	40.0
31	36.559006415052984	39.0	35.0	40.0	31.0	40.0
32	36.418180675174455	38.0	35.0	40.0	31.0	40.0
33	36.11413742822634	38.0	35.0	40.0	31.0	40.0
34	35.90036556511271	38.0	35.0	40.0	30.0	40.0
35	35.88875104242525	38.0	35.0	40.0	30.0	40.0
36	35.87033926734543	38.0	35.0	40.0	30.0	40.0
37	35.50870240796755	38.0	34.0	40.0	29.0	40.0
38	35.538355407620955	37.0	34.0	40.0	30.0	40.0
39	35.142903636235175	37.0	34.0	40.0	28.0	40.0
40	35.062473191799015	37.0	34.0	40.0	28.0	40.0
41	35.01538755965955	37.0	34.0	40.0	28.0	40.0
42	34.91038953741632	36.0	34.0	40.0	28.0	40.0
43	34.77195880257537	36.0	34.0	40.0	27.0	40.0
44	35.070834847017316	36.0	34.0	40.0	29.0	40.0
45	35.182430955148035	36.0	34.0	40.0	29.0	40.0
46	35.07761707847216	36.0	34.0	40.0	29.0	40.0
47	34.768550162420844	35.0	34.0	40.0	28.0	40.0
48	34.68271440580231	35.0	34.0	40.0	28.0	40.0
49	34.606677307424626	35.0	34.0	40.0	28.0	40.0
50	34.09188530012111	35.0	34.0	39.0	26.0	40.0
51	34.176533290013694	35.0	34.0	39.0	26.0	40.0
52	33.814992119571116	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	26.0
16	62.0
17	189.0
18	402.0
19	809.0
20	1355.0
21	2234.0
22	3566.0
23	5473.0
24	7589.0
25	10543.0
26	14332.0
27	19144.0
28	24967.0
29	32317.0
30	40583.0
31	51294.0
32	70386.0
33	110106.0
34	177344.0
35	173116.0
36	185986.0
37	298285.0
38	385527.0
39	181846.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.25173477920056	0.6490157669774147	0.07844267370462495	0.02080678011739697
8	99.07298562273748	0.704537602691752	0.14954177795605098	0.07293499661472574
9	95.50195244371182	2.2783424228549682	1.3308550640865517	0.8888500693466614
10	56.43839106843929	29.200646901709103	5.982950679586156	8.378011350265453
11	33.76066973353413	22.068817034410742	21.712987714542102	22.457525517513023
12	30.729555808624355	14.638849373932889	29.68053803885527	24.951056778587485
13	25.8054699578468	14.67473272467011	29.115806421283892	30.4039908961992
14	22.88250678306595	17.89460976438825	32.80405854605114	26.418824906494663
15	26.5073928047484	16.925536762075584	30.152251110019467	26.414819323156557
16	28.237916073015107	26.148948729089604	25.502881516513852	20.110253681381437
17	31.837767198834378	20.404775322936246	22.777972184561705	24.979485293667675
18	32.83270959353899	18.301120840104346	24.230441209441384	24.63572835691528
19	29.939677027590122	20.729060674017283	23.937254762332607	25.394007536059988
20	30.630250721700413	22.17824734588379	23.402509386695122	23.788992545720674
21	31.79448464554204	18.624738593962363	27.205588233821477	22.375188526674126
22	29.267907321928178	19.059511285452892	25.83773715695934	25.83484423565959
23	29.18006265399938	20.673538838302946	25.00463145573469	25.141767051962987
24	29.77567064313535	20.4208532893906	24.218869524242404	25.584606543231647
25	28.863788469594564	20.03186664077873	22.926234401173637	28.178110488453072
26	27.61159861162031	19.74068298533901	24.714671728537166	27.933046674503515
27	25.858599570178654	18.264069194226842	24.900430655841948	30.976900579752552
28	26.889480948722415	23.142480268329578	22.96873809103912	26.99930069190889
29	31.791758623548045	21.315155402725246	21.413737259324247	25.479348714402466
30	28.93288478217693	21.112205846927743	23.45040948744667	26.504499883448652
31	31.28410656854471	20.66947762186292	23.263760430511198	24.782655379081177
32	32.49651875864747	20.103021378132073	23.646126739994806	23.754333123225653
33	34.06042088666925	19.193809593483362	23.03026830176062	23.715501218086764
34	28.195412383149627	20.82380384658393	25.7779315723973	25.20285219786914
35	26.80736649029119	25.365634654081713	26.399353320823305	21.42764553480379
36	29.623013411471877	24.793281301547545	22.97758375424411	22.606121532736463
37	30.045658086744243	24.98510423696141	22.497025019874926	22.472212656419423
38	30.287383914578708	23.904431232200885	23.0577510541082	22.75043379911221
39	30.7514196176782	21.820526500549935	21.8629745573135	25.565079324458367
40	28.09872205201584	22.374632195654943	22.610572180889918	26.916073571439302
41	25.85654114540768	24.1836537707282	22.862534499477327	27.09727058438679
42	24.754115597797153	22.97090778201393	23.626043190202342	28.648933429986577
43	26.514792007303512	20.816182111621142	24.259370422438835	28.40965545863651
44	25.523688296631246	21.111593882806645	25.250474133111094	28.114243687451008
45	26.65075930839153	21.504141049941282	24.786104631400104	27.05899501026709
46	27.918137003189447	22.782534098918994	23.085511971965367	26.213816925926192
47	27.626174484322867	21.472485814949838	25.103880909556707	25.79745879117058
48	28.019055450069015	20.78246845185873	25.70304941721544	25.495426680856813
49	27.971656047234728	22.51098892845639	24.46259814374592	25.054756880562962
50	24.357062149407145	22.838612265652515	25.366580416814326	27.437745168126014
51	25.522297469083295	23.093133706928157	24.94120971954797	26.443359104440578
52	26.400577249065506	23.231437598296736	24.797843215904834	25.570141936732927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.0
10	8.0
11	68.5
12	129.0
13	506.5
14	1988.5
15	3093.0
16	3972.5
17	4852.0
18	4972.5
19	5093.0
20	5246.5
21	5400.0
22	5908.0
23	6416.0
24	6383.0
25	6350.0
26	6076.5
27	5803.0
28	7334.5
29	8866.0
30	9574.5
31	10283.0
32	10726.5
33	11170.0
34	11651.5
35	12133.0
36	14703.5
37	17274.0
38	18641.0
39	23929.5
40	27851.0
41	30286.0
42	32721.0
43	37485.5
44	42250.0
45	39724.5
46	37199.0
47	44454.5
48	51710.0
49	56073.5
50	60437.0
51	63581.0
52	66725.0
53	74568.0
54	82411.0
55	93168.5
56	103926.0
57	114018.0
58	124110.0
59	140588.5
60	157067.0
61	168348.0
62	179629.0
63	172147.5
64	160545.5
65	156425.0
66	139303.5
67	122182.0
68	105886.5
69	89591.0
70	73759.5
71	57928.0
72	51300.0
73	44672.0
74	37999.0
75	31326.0
76	28011.5
77	24697.0
78	19816.5
79	14936.0
80	9975.5
81	5015.0
82	3268.0
83	1521.0
84	1088.0
85	655.0
86	348.5
87	42.0
88	27.5
89	9.0
90	5.0
91	6.5
92	8.0
93	6.5
94	5.0
95	5.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1797491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.248175698355423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69490070399007	7.347419390906361
2	9.842803618157191	2.0174156168656676
3	4.06705615449985	1.2503971813917667
4	2.07568712625165	0.8508802545856545
5	1.2124161482457327	0.6212526853372802
6	0.7892729272618128	0.4853164579520614
7	0.5297880924410919	0.38005530179630115
8	0.3510135280707193	0.28777986465346755
9	0.2748770285462563	0.2535289275585504
>10	3.93838368514074	10.914175176065987
>50	2.837096595494641	21.028342134650963
>100	2.2544562656371223	40.18003845798381
>500	0.09294797553401528	6.32075789510589
>1k	0.038052526896476056	6.862431203206669
>5k	6.238119163356731E-4	0.4608026914658824
>10k+	6.238119163356731E-4	0.7394067604736952
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11566	0.6434524567855973	No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7208	0.40100339862619616	No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGAAG	4246	0.23621815074456565	No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4043	0.22492463105517635	No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3969	0.2208077815132315	No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3870	0.2153001044233323	No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3260	0.18136391225324633	No Hit
GCCTAGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	3037	0.1689577305254936	No Hit
GCCTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2824	0.1571078798169226	No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCA	2812	0.15644028259390452	No Hit
GCCTAGGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC	2803	0.15593958467664096	No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2710	0.15076570619825078	No Hit
GCCTAGGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	2436	0.13552223627267118	No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC	2318	0.1289575302463267	No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2198	0.12228155801614583	No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2136	0.11883230569721906	No Hit
GCCTAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2100	0.11682951402816483	No Hit
GCCTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2086	0.11605065060131038	No Hit
GCCTAGGGGAAATTAAAGGAGCATCCCATCTTCTTCATGTTCATTCAGATTG	2084	0.11593938439747405	No Hit
GCCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1946	0.10826201633276607	No Hit
GCCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1925	0.1070937211924844	No Hit
GCCTAGGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC	1817	0.10108534618532164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.021863809053842272	0.0	0.0	0.0	0.0
6	0.021863809053842272	0.0	0.0	0.0	0.0
7	0.021919442155760447	0.0	0.0	0.0	0.0
8	0.022141974563433142	0.0	0.0	0.0	0.0
9	0.024033500028651046	0.0	0.0	0.0	0.0
10	0.038164307915867175	0.0	0.0	0.0	0.0
11	0.04934656140142009	0.0	0.0	0.0	0.0
12	0.0541866412683012	0.0	0.0	0.0	0.0
13	0.05974995146011858	0.0	0.0	0.0	0.0
14	0.09090448853429586	0.0	0.0	0.0	0.0
15	0.09886002210859471	0.0	0.0	0.0	0.0
16	0.11465982305335604	0.0	0.0	0.0	0.0
17	0.1276223358002905	0.0	0.0	0.0	0.0
18	0.1371912293302164	0.0	0.0	0.0	0.0
19	0.14748335318507855	0.0	0.0	0.0	0.0
20	0.15721914602075893	0.0	0.0	0.0	0.0
21	0.16656550714301213	0.0	0.0	0.0	0.0
22	0.17502173863457451	0.0	0.0	0.0	0.0
23	0.18687158934314552	0.0	0.0	0.0	0.0
24	0.19916650486706192	0.0	0.0	0.0	0.0
25	0.20478544816079747	0.0	0.0	0.0	0.0
26	0.21290788104085084	0.0	0.0	0.0	0.0
27	0.22275494008036759	0.0	0.0	0.0	0.0
28	0.2648691982324251	0.0	0.0	0.0	0.0
29	0.27193460217603316	0.0	0.0	0.0	0.0
30	0.28656610798051285	0.0	0.0	0.0	0.0
31	0.29479980706440256	0.0	0.0	0.0	0.0
32	0.3042018012885739	0.0	0.0	0.0	0.0
33	0.31026580939765486	0.0	0.0	0.0	0.0
34	0.31922273880648083	0.0	0.0	0.0	0.0
35	0.3709058904884642	0.0	0.0	0.0	0.0
36	0.3906000085674977	0.0	0.0	0.0	0.0
37	0.40044706760701443	0.0	0.0	0.0	0.0
38	0.4087920328947405	0.0	0.0	0.0	0.0
39	0.4175264298958938	0.0	0.0	0.0	0.0
40	0.42475873314525636	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTA	35	1.0199983E-7	46.000004	21
ATCCGAT	35	1.0199983E-7	46.000004	28
CAACGAC	25	3.417931E-5	46.0	38
ACCGATT	25	3.417931E-5	46.0	12
TTCGTAC	25	3.417931E-5	46.0	22
TAACGCG	45	3.110472E-10	46.0	10
TAACGAT	20	6.3124095E-4	46.0	35
TTATCGG	30	1.861994E-6	46.0	14
CTAACGC	40	5.6152203E-9	46.0	17
CCCGTAA	25	3.417931E-5	46.0	39
AAACCGG	20	6.3124095E-4	46.0	26
AACGATA	20	6.3124095E-4	46.0	17
TCCGTAT	25	3.417931E-5	46.0	40
ACGTCTA	25	3.417931E-5	46.0	34
ACGTCCC	20	6.3124095E-4	46.0	19
CGTCGAT	30	1.861994E-6	46.0	40
TTACGAC	30	1.861994E-6	46.0	12
CCGAACT	25	3.417931E-5	46.0	46
CGCGTAT	40	5.6152203E-9	46.0	39
TAATGCG	20	6.3124095E-4	46.0	19
>>END_MODULE