FastQCFastQC Report
Fri 17 Jun 2016
SRR1527136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743974
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC108820.6239771923205277No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71860.4120474273125631No Hit
GCCTAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41730.2392810901997392No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCCTAGGGGAAG41660.23887970806904232No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT38220.2191546433605088No Hit
GCCTAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG37630.21577156540177775No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA32070.18389035616356667No Hit
GCCTAGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG28380.16273178384540135No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCA28020.16066753288753158No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT26490.151894466316585No Hit
GCCTAGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26260.15057563931572374No Hit
GCCTAGGGGAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAAACCCATAC26080.14954351383678885No Hit
GCCTAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC22240.12752483695284447No Hit
GCCTAGGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT21790.12494452325550726No Hit
GCCTAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21240.1217908065143173No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20940.12007059738275916No Hit
GCCTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20700.11869443007751262No Hit
GCCTAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA20450.11726092246788083No Hit
GCCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG19270.11049476655041876No Hit
GCCTAGGGGAAATTAAAGGAGCATCCCATCTTCTTCATGTTCATTCAGATTG19020.10906125894078697No Hit
GCCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA18040.10344190911103032No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT206.3123566E-446.00000410
TATCTAG206.3123566E-446.00000412
CGTACTT351.0199801E-746.00000410
ACTAACG206.3123566E-446.00000423
ACGTATA206.3123566E-446.00000438
TATCGCG206.3123566E-446.00000426
ATCGCGA206.3123566E-446.00000410
GTTACGT551.8189894E-1246.046
TTACGCG253.4178884E-546.040
TTACGAC301.8619612E-646.012
CCGCGTA253.4178884E-546.044
TCGACGG253.4178884E-546.016
TACGTAT750.046.017
TTAACCG1050.043.80952532
ACGTATT800.043.12500418
TAGGGGG983300.043.1159364
CCTAGGG1836250.043.0602572
AGGGGAT163750.043.0363355
GCCTAGG1852550.043.0141141
CTAGGGG1760550.042.961293
Produced by FastQC (version 0.11.5)