FastQCFastQC Report
Fri 17 Jun 2016
SRR1527135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109960
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGAT69120.3275891486094523No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT38890.18431629035621527No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGT38580.18284706819086616No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT33750.15995563896945914No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAG31900.15118770024076286No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC31820.150808546133576No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTT30610.14507384026237463No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28810.13654287285067016No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC28580.1354528047925079No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGC26070.12355684467951998No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGT25280.11981269787104969No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC23570.11170827882993042No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC23500.11137651898614193No Hit
TTCATAGGGACGTGGGATGGAGTTCTAACCTCATAGGGTGGTGGTGGGGA22530.10677927543650116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG408.3182385E-944.00000439
TAGCGAT207.857979E-444.00000429
CGCGCAT207.857979E-444.00000434
CGTCTAT207.857979E-444.00000433
TAACGTA207.857979E-444.00000432
ACTAGCG207.857979E-444.00000427
TACGCGT207.857979E-444.00000423
CGAATTG207.857979E-444.00000428
CGTACGT950.044.00000427
CTACCGA207.857979E-444.00000444
TACCGCG408.3182385E-944.00000441
ACGAACG650.044.00000410
CGTATTG1000.044.020
CACGATT302.5287281E-644.024
TAACGAT254.4441105E-544.043
CGTTAGT351.4468606E-744.032
CGTTACG302.5287281E-644.012
ACGATAC254.4441105E-544.035
TTACGTA1000.044.024
ACGTACG900.044.026
Produced by FastQC (version 0.11.5)