##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109960
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48711539555252	33.0	31.0	34.0	31.0	34.0
2	32.87821475288631	34.0	31.0	34.0	31.0	34.0
3	32.99801702401941	34.0	31.0	34.0	31.0	34.0
4	36.32303171624107	37.0	37.0	37.0	35.0	37.0
5	36.329880661244765	37.0	37.0	37.0	35.0	37.0
6	36.291711691216896	37.0	37.0	37.0	35.0	37.0
7	36.518244895637835	37.0	37.0	37.0	35.0	37.0
8	36.672278147453035	37.0	37.0	37.0	35.0	37.0
9	38.758171244952514	39.0	39.0	39.0	39.0	39.0
10	38.23309114864737	39.0	39.0	39.0	37.0	39.0
11	37.982101082484974	39.0	38.0	39.0	35.0	39.0
12	37.71231113386036	39.0	37.0	39.0	35.0	39.0
13	37.58195273844054	39.0	37.0	39.0	35.0	39.0
14	38.46376898140249	40.0	38.0	40.0	35.0	40.0
15	38.45007772659197	40.0	38.0	40.0	35.0	40.0
16	38.34186145708924	40.0	38.0	40.0	35.0	40.0
17	38.41976151206658	40.0	38.0	40.0	35.0	40.0
18	38.38821352063546	40.0	38.0	40.0	35.0	40.0
19	38.413341011204004	40.0	38.0	40.0	35.0	40.0
20	38.20405078769266	40.0	38.0	40.0	34.0	40.0
21	38.38333996853021	40.0	38.0	40.0	35.0	40.0
22	38.218696089025386	40.0	38.0	40.0	34.0	40.0
23	38.29509421979564	40.0	38.0	40.0	35.0	40.0
24	38.319514587954274	40.0	38.0	40.0	35.0	40.0
25	38.25517877116154	40.0	38.0	40.0	35.0	40.0
26	38.1450880585414	40.0	38.0	40.0	34.0	40.0
27	38.042655311001155	40.0	38.0	40.0	34.0	40.0
28	37.936504483497316	40.0	38.0	40.0	34.0	40.0
29	38.106445619822175	40.0	38.0	40.0	34.0	40.0
30	38.097226487705925	40.0	38.0	40.0	34.0	40.0
31	38.095252516635384	40.0	38.0	40.0	35.0	40.0
32	37.37341181823352	40.0	37.0	40.0	33.0	40.0
33	37.61671785247114	40.0	37.0	40.0	33.0	40.0
34	37.650070617452464	40.0	37.0	40.0	33.0	40.0
35	37.68397694743028	40.0	37.0	40.0	33.0	40.0
36	37.48171908472199	40.0	37.0	40.0	33.0	40.0
37	37.48495089954312	40.0	37.0	40.0	33.0	40.0
38	37.31170069574779	40.0	37.0	40.0	33.0	40.0
39	37.28877561659937	40.0	36.0	40.0	33.0	40.0
40	37.21276659273162	40.0	36.0	40.0	33.0	40.0
41	37.15524701890083	40.0	36.0	40.0	33.0	40.0
42	37.028392481374055	40.0	35.0	40.0	33.0	40.0
43	36.9356689226336	40.0	35.0	40.0	32.0	40.0
44	36.8270374793835	40.0	35.0	40.0	32.0	40.0
45	36.727559764166145	40.0	35.0	40.0	32.0	40.0
46	36.45005355551764	39.0	35.0	40.0	31.0	40.0
47	36.42086153292006	39.0	35.0	40.0	31.0	40.0
48	36.329292024493355	39.0	35.0	40.0	31.0	40.0
49	35.98585186449032	39.0	35.0	40.0	30.0	40.0
50	35.63254990615936	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	6.0
15	6.0
16	32.0
17	89.0
18	173.0
19	377.0
20	630.0
21	1061.0
22	1765.0
23	2758.0
24	4132.0
25	6019.0
26	8208.0
27	10828.0
28	14903.0
29	19466.0
30	25568.0
31	33238.0
32	41987.0
33	55931.0
34	92258.0
35	120312.0
36	154867.0
37	286628.0
38	694703.0
39	534010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.66838233900168	0.2662609717719767	0.02801000966842973	0.03734667955790631
8	99.55800109954691	0.37697397107054165	0.043744905116684676	0.021280024265862858
9	98.23868698932681	0.9820565318773816	0.2246014142448198	0.5546550645509868
10	45.99224629850803	42.70142561944302	4.083394945875751	7.222933136173197
11	29.511981269787107	23.06470264839144	29.664116855295834	17.75919922652562
12	29.04040834897344	17.272744507004873	28.86803541299361	24.818811731028077
13	23.33233805380197	17.925884850897646	32.315067584219605	26.42670951108078
14	26.797569622172933	19.44979999620846	30.326309503497693	23.42632087812091
15	25.023602343172378	20.506218127357865	29.39401694818859	25.076162581281164
16	27.941856717662894	22.57426681074523	27.360139528711446	22.123736942880434
17	29.08742345826461	20.393941117367152	24.369324536958047	26.149310887410188
18	24.96796147794271	21.129405296782878	27.89792223549262	26.0047109897818
19	25.551621831693495	21.174572029801514	27.391419742554362	25.882386395950636
20	24.255625699065387	23.17385163699786	28.17195586646192	24.39856679747483
21	26.162865646742116	20.99945022654458	29.563309257047525	23.274374869665778
22	25.864850518493242	21.19400367779484	28.066787995980967	24.874357807730952
23	23.880642287057576	22.38141955297731	29.90905040853855	23.828887751426567
24	24.734307759388805	23.097926027033687	26.54036095471004	25.62740525886747
25	23.951638893628317	26.24599518474284	25.523943581868853	24.278422339759995
26	26.104096760128154	24.93388500255929	25.838688885097348	23.123329352215208
27	26.162154732791144	23.45589489848149	25.461572731236608	24.92037763749076
28	28.383571252535596	22.227530379722836	25.033175984378854	24.355722383362718
29	27.554029460274126	22.94896585717265	24.534967487535308	24.962037195017913
30	26.64576579650799	23.18849646438795	25.815465696032152	24.350272043071907
31	27.51824679140837	24.210221994729757	24.506483535232896	23.765047678628978
32	26.94425486739085	24.25993857703464	24.749616106466473	24.04619044910804
33	27.60308252289143	23.07527156912927	24.948956378319966	24.37268952965933
34	25.244364822081934	25.21464861893116	26.332821475288632	23.20816508369827
35	25.801531782593035	25.177444122163458	26.85069859144249	22.17032550380102
36	26.229075432709624	25.598873912301656	25.36062295019811	22.811427704790614
37	27.912567062882708	26.45998028398643	23.312716828755047	22.314735824375816
38	25.63484615822101	26.397372462037193	25.192278526607137	22.775502853134657
39	26.06641832072646	25.51825627026105	24.664685586456613	23.75063982255588
40	25.32597774365391	26.03992492748678	24.95928832774081	23.674809001118504
41	25.07876926576807	25.60536692638723	25.35337162789816	23.96249217994654
42	23.657415306451306	24.494682363646707	26.737426301920415	25.11047602798157
43	24.497004682553225	25.42261464672316	26.113717795598017	23.966662875125593
44	24.25372992852945	25.380338963771827	25.500625604276863	24.865305503421865
45	24.082447060607784	24.942415969970995	25.801579176856432	25.17355779256479
46	25.248156363153807	25.399438851921364	25.303465468539688	24.048939316385145
47	24.14609755635178	25.79736108741398	25.947600902386775	24.108940453847467
48	25.515744374300937	25.232184496388555	25.168818366224954	24.083252763085554
49	24.753928984435724	26.36049972511327	25.067347248289067	23.818224042161937
50	25.150524180553184	26.006985914424916	25.28498170581433	23.557508199207568
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	7.5
2	15.0
3	10.5
4	6.0
5	72.5
6	139.0
7	1023.5
8	1908.0
9	4734.5
10	7561.0
11	8690.5
12	9820.0
13	9443.0
14	9066.0
15	9216.5
16	9367.0
17	9819.5
18	10272.0
19	10327.5
20	10383.0
21	11688.0
22	12993.0
23	15363.5
24	17734.0
25	20094.0
26	22454.0
27	27247.0
28	32040.0
29	34298.0
30	36556.0
31	45272.0
32	53988.0
33	51171.0
34	48354.0
35	56136.0
36	63918.0
37	64463.0
38	65008.0
39	72231.0
40	79454.0
41	81798.5
42	84143.0
43	97522.0
44	110901.0
45	112291.0
46	113681.0
47	120419.5
48	127158.0
49	133354.5
50	139551.0
51	145156.5
52	150762.0
53	155464.0
54	160166.0
55	159327.0
56	158488.0
57	156039.0
58	153590.0
59	140385.5
60	127181.0
61	115256.0
62	103331.0
63	85043.0
64	66755.0
65	52729.5
66	38704.0
67	35443.0
68	32182.0
69	27402.5
70	22623.0
71	19333.5
72	16044.0
73	11744.0
74	7444.0
75	5046.5
76	2649.0
77	2574.0
78	2499.0
79	1648.5
80	798.0
81	499.5
82	201.0
83	136.0
84	71.0
85	36.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2109960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.658174542133825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.96485615406414	5.051703820745737
2	10.919497739977423	1.6724683921036587
3	4.917093397741552	1.1296787843963598
4	2.566871543956751	0.7863020124342935
5	1.5265313207150581	0.5845221649034991
6	0.9447033514743153	0.4340821893677463
7	0.6290454949992524	0.3372138136953071
8	0.4588949198138818	0.281143791394657
9	0.35513399538131113	0.24477103102278855
>10	4.641334790936156	9.605771701970538
>50	3.436747512773961	19.217286805702663
>100	3.457454709235789	47.611311356398645
>500	0.13783227644904042	7.104997978782296
>1k	0.04335569259195168	5.596214815550685
>5k	6.470998894321147E-4	0.34253134153113374
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGAT	6912	0.3275891486094523	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3889	0.18431629035621527	No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGT	3858	0.18284706819086616	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3375	0.15995563896945914	No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAG	3190	0.15118770024076286	No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3182	0.150808546133576	No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTT	3061	0.14507384026237463	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2881	0.13654287285067016	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2858	0.1354528047925079	No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGC	2607	0.12355684467951998	No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGT	2528	0.11981269787104969	No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2357	0.11170827882993042	No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2350	0.11137651898614193	No Hit
TTCATAGGGACGTGGGATGGAGTTCTAACCTCATAGGGTGGTGGTGGGGA	2253	0.10677927543650116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.007583082143737322	0.0	0.0	0.0	0.0
7	0.007583082143737322	0.0	0.0	0.0	0.0
8	0.007962236250924189	0.0	0.0	0.0	0.0
9	0.008341390358111054	0.0	0.0	0.0	0.0
10	0.013412576541735388	0.0	0.0	0.0	0.0
11	0.023270583328593906	0.0	0.0	0.0	0.0
12	0.029621414623973913	0.0	0.0	0.0	0.0
13	0.03601964018275228	0.0	0.0	0.0	0.0
14	0.048958274090504086	0.0	0.0	0.0	0.0
15	0.058579309560370815	0.0	0.0	0.0	0.0
16	0.07180230904851277	0.0	0.0	0.0	0.0
17	0.07848490018768128	0.0	0.0	0.0	0.0
18	0.0868736848091907	0.0	0.0	0.0	0.0
19	0.09715823996663443	0.0	0.0	0.0	0.0
20	0.10403040815939639	0.0	0.0	0.0	0.0
21	0.11208743293711729	0.0	0.0	0.0	0.0
22	0.12057100608542343	0.0	0.0	0.0	0.0
23	0.12919676202392463	0.0	0.0	0.0	0.0
24	0.1373959695918406	0.0	0.0	0.0	0.0
25	0.14497905173557793	0.0	0.0	0.0	0.0
26	0.15151946008455136	0.0	0.0	0.0	0.0
27	0.15900775370149198	0.0	0.0	0.0	0.0
28	0.16758611537659482	0.0	0.0	0.0	0.0
29	0.17493222620334034	0.0	0.0	0.0	0.0
30	0.1846480502000038	0.0	0.0	0.0	0.0
31	0.19270507497772468	0.0	0.0	0.0	0.0
32	0.20095167680903903	0.0	0.0	0.0	0.0
33	0.20919827864035337	0.0	0.0	0.0	0.0
34	0.2192932567442037	0.0	0.0	0.0	0.0
35	0.23043090864281787	0.0	0.0	0.0	0.0
36	0.24237426301920414	0.0	0.0	0.0	0.0
37	0.2515213558550873	0.0	0.0	0.0	0.0
38	0.2611897855883524	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	8.3182385E-9	44.000004	39
TAGCGAT	20	7.857979E-4	44.000004	29
CGCGCAT	20	7.857979E-4	44.000004	34
CGTCTAT	20	7.857979E-4	44.000004	33
TAACGTA	20	7.857979E-4	44.000004	32
ACTAGCG	20	7.857979E-4	44.000004	27
TACGCGT	20	7.857979E-4	44.000004	23
CGAATTG	20	7.857979E-4	44.000004	28
CGTACGT	95	0.0	44.000004	27
CTACCGA	20	7.857979E-4	44.000004	44
TACCGCG	40	8.3182385E-9	44.000004	41
ACGAACG	65	0.0	44.000004	10
CGTATTG	100	0.0	44.0	20
CACGATT	30	2.5287281E-6	44.0	24
TAACGAT	25	4.4441105E-5	44.0	43
CGTTAGT	35	1.4468606E-7	44.0	32
CGTTACG	30	2.5287281E-6	44.0	12
ACGATAC	25	4.4441105E-5	44.0	35
TTACGTA	100	0.0	44.0	24
ACGTACG	90	0.0	44.0	26
>>END_MODULE