FastQCFastQC Report
Fri 17 Jun 2016
SRR1527134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233475
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT61690.2762063600443255No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGTGG36980.16557158687695184No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35520.15903468809814303No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAGAC33390.14949797960577127No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32040.14345358689933846No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT28370.12702179339370265No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC27360.12249969218370475No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTTTG26820.12008193510113165No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGCCA26360.11802236425301381No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26180.11721644522548942No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC24100.10790360312965222No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC23550.10544107276777219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT1450.046.00000427
CAATTCG206.3127396E-446.032
TAAGTCG650.046.032
TTATCCG253.4181983E-546.039
CGTTAGG253.4181983E-546.044
TAACACG206.3127396E-446.041
ACGTACG1350.046.026
GACGAAT206.3127396E-446.09
TATACGC253.4181983E-546.035
CGATCGT253.4181983E-546.026
TATAACG253.4181983E-546.023
ACGAACG253.4181983E-546.010
CGTATTG1500.044.46666320
TTACGTA1400.044.35714324
TAGGGAT371550.043.4186485
AGGGATC97400.043.2371646
TACGTAC1500.042.9333325
CGCATAC700.042.71428723
TGACGTA700.042.71428725
ATAGGGA1003000.042.5488554
Produced by FastQC (version 0.11.5)