##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2233475
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.431468003895276	33.0	31.0	34.0	31.0	34.0
2	32.894715185977006	34.0	31.0	34.0	31.0	34.0
3	32.90158788435062	34.0	31.0	34.0	31.0	34.0
4	36.239563012793965	37.0	37.0	37.0	35.0	37.0
5	36.29726278556957	37.0	37.0	37.0	35.0	37.0
6	36.29949562900861	37.0	37.0	37.0	35.0	37.0
7	36.50266871131309	37.0	37.0	37.0	35.0	37.0
8	36.62959200349231	37.0	37.0	37.0	35.0	37.0
9	38.72289548797278	39.0	39.0	39.0	39.0	39.0
10	38.12498460918524	39.0	39.0	39.0	37.0	39.0
11	37.863330012648454	39.0	38.0	39.0	35.0	39.0
12	37.628173586003875	39.0	37.0	39.0	35.0	39.0
13	37.538641354839434	39.0	37.0	39.0	35.0	39.0
14	38.437019890529335	40.0	38.0	40.0	35.0	40.0
15	38.324896405825	40.0	38.0	40.0	35.0	40.0
16	33.21966979706511	40.0	35.0	40.0	3.0	40.0
17	34.9939793371316	40.0	35.0	40.0	17.0	40.0
18	37.078783062268435	40.0	35.0	40.0	32.0	40.0
19	37.98224291742688	40.0	37.0	40.0	34.0	40.0
20	38.22569269859748	40.0	38.0	40.0	34.0	40.0
21	38.41800467880769	40.0	38.0	40.0	35.0	40.0
22	38.45582645876941	40.0	38.0	40.0	35.0	40.0
23	38.41766708828171	40.0	38.0	40.0	35.0	40.0
24	38.3763373218863	40.0	38.0	40.0	35.0	40.0
25	38.30641086199756	40.0	38.0	40.0	35.0	40.0
26	38.22653264531728	40.0	38.0	40.0	35.0	40.0
27	38.11502165907387	40.0	38.0	40.0	34.0	40.0
28	38.06186368775115	40.0	38.0	40.0	34.0	40.0
29	37.86942768555726	40.0	38.0	40.0	34.0	40.0
30	37.84575336639094	40.0	38.0	40.0	34.0	40.0
31	37.83718868579232	40.0	37.0	40.0	34.0	40.0
32	37.77911416066891	40.0	37.0	40.0	34.0	40.0
33	37.461712801799884	40.0	37.0	40.0	33.0	40.0
34	37.45763932884854	40.0	37.0	40.0	33.0	40.0
35	37.42340836588724	40.0	37.0	40.0	33.0	40.0
36	37.34323509329632	40.0	36.0	40.0	33.0	40.0
37	37.07738792688524	40.0	36.0	40.0	32.0	40.0
38	37.10410593357884	40.0	36.0	40.0	32.0	40.0
39	36.71908841603331	39.0	35.0	40.0	31.0	40.0
40	36.74921590794614	39.0	35.0	40.0	31.0	40.0
41	36.78359417499636	39.0	35.0	40.0	32.0	40.0
42	36.68820559889858	39.0	35.0	40.0	31.0	40.0
43	36.59336639093789	39.0	35.0	40.0	31.0	40.0
44	36.82104344127425	39.0	35.0	40.0	32.0	40.0
45	36.85646358253394	39.0	35.0	40.0	32.0	40.0
46	36.80604349724085	39.0	35.0	40.0	32.0	40.0
47	36.51543670737304	39.0	35.0	40.0	31.0	40.0
48	36.48565710384043	39.0	35.0	40.0	31.0	40.0
49	36.36906390266289	39.0	35.0	40.0	31.0	40.0
50	35.899521597510606	39.0	35.0	40.0	30.0	40.0
51	35.97381703399411	38.0	35.0	40.0	30.0	40.0
52	35.6287793684729	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	11.0
16	21.0
17	89.0
18	138.0
19	283.0
20	563.0
21	1061.0
22	1779.0
23	2821.0
24	4401.0
25	6466.0
26	9021.0
27	12613.0
28	17356.0
29	22563.0
30	30252.0
31	38952.0
32	51844.0
33	73725.0
34	109099.0
35	150642.0
36	228329.0
37	422810.0
38	626567.0
39	422065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.70494408936746	0.24383527910542988	0.015312461522963094	0.035908170004141524
8	99.54555121503486	0.3791849024502177	0.05274292302353955	0.022520959491375548
9	98.2400967102833	0.9754306629803333	0.21768768399019464	0.5667849427461691
10	46.18372715163591	42.37732681135898	4.146095210378446	7.292850826626669
11	29.62616550442696	23.029852583977885	29.53666371909244	17.807318192502713
12	29.162582970483214	17.33016935492898	28.730296958775003	24.776950715812802
13	23.39641142166355	17.870941022397833	32.29111586205352	26.44153169388509
14	26.913039098266157	19.393904118022366	30.127760552502263	23.565296231209214
15	25.094482812657404	20.69340377662611	29.140957476577977	25.071155934138506
16	26.67009928474686	30.05943652827992	24.001790931172277	19.268673255800937
17	29.066096553576827	20.491118100717493	24.350216590738647	26.09256875496704
18	25.24948790561793	21.115391934093733	27.648484984161453	25.98663517612689
19	25.590302107702122	21.17628359395113	27.284254356999742	25.949159941347006
20	24.27396769607898	23.13900983892813	28.134722797434492	24.4522996675584
21	26.260647645485175	20.998488901823393	29.460996877063767	23.279866575627665
22	25.68441554080525	21.165896193151926	28.059145501964426	25.0905427640784
23	24.012536518205934	22.375267240510862	29.780812411153022	23.83138383013018
24	24.818186906054468	23.097146822776168	26.49378211083625	25.59088416033311
25	24.11721644522549	26.108239402724454	25.461981889208516	24.312562262841535
26	26.30201815556476	24.62534839207961	25.809646403026676	23.26298704932896
27	26.23315685199073	23.45784036087263	25.30899159381681	25.00001119331983
28	28.461254323419784	22.194875698183324	24.902494990989375	24.441374987407517
29	27.842039870605223	22.74966140207524	24.519190946842926	24.88910778047661
30	26.71711122801912	23.10825059604428	25.850569180313187	24.324068995623414
31	27.689049575213513	24.1386628460135	24.394004857900807	23.778282720872184
32	26.91921781081051	24.03460974490424	24.75219109235608	24.29398135192917
33	27.87306775316491	23.003928855259183	24.88798844849394	24.23501494308197
34	25.424372334590718	24.995399545551216	26.27725853210804	23.30296958775003
35	25.92475850412474	25.21017696638646	26.749616628795934	22.115447900692867
36	26.18121984799472	25.674341552961195	25.33388553711145	22.81055306193264
37	28.248760339829186	26.31518149968099	23.26531525985292	22.1707429006369
38	25.65002966229754	26.46203785580765	24.996250237858046	22.891682244036758
39	26.474171414499825	25.451773581526542	24.565979023718647	23.508075980254983
40	25.49529320901286	25.991425917012727	24.79463616113903	23.718644712835378
41	25.160254759959255	25.58815299029539	25.290947962256126	23.960644287489227
42	23.686855684527476	24.367722942947648	26.629848106649952	25.315573265874924
43	24.501908461030457	25.344272937910656	26.109806467500196	24.04401213355869
44	24.323128756757967	25.31391665454057	25.432207658469423	24.930746930232036
45	24.181600420868826	24.782323509329633	25.807004779547565	25.229071290253973
46	25.037396881541095	25.322916083681257	25.33361689743561	24.306070137342033
47	24.393736218224962	25.74338194965245	25.787752269445598	24.075129562676995
48	25.657148613707335	25.221773245726954	25.104064294429087	24.017013846136624
49	24.76553352958954	26.431547252599646	24.928911225780453	23.874007992030354
50	25.18134297451281	25.99182887652649	25.18080569516113	23.646022453799574
51	23.964450016230312	26.981721308722957	24.375020987474674	24.678807687572057
52	24.977714100224986	26.98498975811236	23.718823805952606	24.318472335710048
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	16.0
2	32.0
3	17.5
4	3.0
5	62.0
6	121.0
7	1054.5
8	1988.0
9	4822.0
10	7656.0
11	8962.5
12	10269.0
13	9884.5
14	9446.0
15	9392.0
16	9863.5
17	10335.0
18	10005.5
19	9676.0
20	10707.5
21	11739.0
22	13867.5
23	15996.0
24	16514.5
25	17033.0
26	23977.5
27	30922.0
28	32397.5
29	33873.0
30	38724.0
31	43575.0
32	44694.5
33	45814.0
34	50894.0
35	55974.0
36	61549.0
37	67124.0
38	68653.0
39	75052.0
40	79922.0
41	85364.5
42	90807.0
43	100067.0
44	109327.0
45	112952.0
46	116577.0
47	121376.0
48	126175.0
49	135585.0
50	144995.0
51	148337.5
52	151680.0
53	157682.0
54	163684.0
55	169392.0
56	175100.0
57	162009.5
58	148919.0
59	140528.0
60	132137.0
61	125092.0
62	118047.0
63	97716.0
64	63757.5
65	50130.0
66	41459.5
67	32789.0
68	29761.5
69	26734.0
70	24307.0
71	21880.0
72	14566.0
73	7252.0
74	6021.5
75	4791.0
76	3421.0
77	2051.0
78	1649.0
79	1247.0
80	889.5
81	532.0
82	293.0
83	54.0
84	51.0
85	48.0
86	26.0
87	4.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2233475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.505690525164077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41746692931916	5.819378002164366
2	10.157023416344973	1.7278499567255023
3	4.50633300231013	1.1498842176295039
4	2.3487505452251938	0.7991098103398357
5	1.3577597899839877	0.5774342290557787
6	0.8211252614130042	0.419054241358408
7	0.5796025673160893	0.3450944045626864
8	0.40176995719529707	0.27338647345692924
9	0.3156058299589594	0.24160009658116197
>10	4.621586575566502	10.674300452842079
>50	3.283553139326737	20.29622151737261
>100	3.038447999924276	45.78512599695384
>500	0.11613460470440806	6.594950154077685
>1k	0.034229146649720266	4.975885758067505
>5k	6.112347616021477E-4	0.32072468881211214
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT	6169	0.2762063600443255	No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGTGG	3698	0.16557158687695184	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3552	0.15903468809814303	No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAGAC	3339	0.14949797960577127	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3204	0.14345358689933846	No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2837	0.12702179339370265	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2736	0.12249969218370475	No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTTTG	2682	0.12008193510113165	No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGCCA	2636	0.11802236425301381	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2618	0.11721644522548942	No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC	2410	0.10790360312965222	No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2355	0.10544107276777219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.009626255050985572	0.0	0.0	0.0	0.0
7	0.009715801609599391	0.0	0.0	0.0	0.0
8	0.010073987844054668	0.0	0.0	0.0	0.0
9	0.010432174078509946	0.0	0.0	0.0	0.0
10	0.015267688243656185	0.0	0.0	0.0	0.0
11	0.02337165179820683	0.0	0.0	0.0	0.0
12	0.028475805639194527	0.0	0.0	0.0	0.0
13	0.033669506038796045	0.0	0.0	0.0	0.0
14	0.04611647768611692	0.0	0.0	0.0	0.0
15	0.055026360268191944	0.0	0.0	0.0	0.0
16	0.0683240242223441	0.0	0.0	0.0	0.0
17	0.07593548170451875	0.0	0.0	0.0	0.0
18	0.08408421853837629	0.0	0.0	0.0	0.0
19	0.0929493278411444	0.0	0.0	0.0	0.0
20	0.10114283795430887	0.0	0.0	0.0	0.0
21	0.1087095221571766	0.0	0.0	0.0	0.0
22	0.11855964360469672	0.0	0.0	0.0	0.0
23	0.12626064764548517	0.0	0.0	0.0	0.0
24	0.1343198379207289	0.0	0.0	0.0	0.0
25	0.14143878933052753	0.0	0.0	0.0	0.0
26	0.1500352589574542	0.0	0.0	0.0	0.0
27	0.157064663808639	0.0	0.0	0.0	0.0
28	0.16575067999417947	0.0	0.0	0.0	0.0
29	0.17448146945902684	0.0	0.0	0.0	0.0
30	0.18361521843763642	0.0	0.0	0.0	0.0
31	0.1923460079024838	0.0	0.0	0.0	0.0
32	0.20085293097079662	0.0	0.0	0.0	4.477327930690964E-5
33	0.208598708290892	0.0	0.0	0.0	4.477327930690964E-5
34	0.21526992690762153	0.0	0.0	0.0	4.477327930690964E-5
35	0.22601551394127983	0.0	0.0	0.0	4.477327930690964E-5
36	0.23846248558860073	0.0	0.0	0.0	4.477327930690964E-5
37	0.247238048332755	0.0	0.0	0.0	4.477327930690964E-5
38	0.25699862322166134	0.0	0.0	0.0	4.477327930690964E-5
39	0.26559509284858795	0.0	0.0	0.0	4.477327930690964E-5
40	0.27441542887204917	0.0	0.0	0.0	4.477327930690964E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	145	0.0	46.000004	27
CAATTCG	20	6.3127396E-4	46.0	32
TAAGTCG	65	0.0	46.0	32
TTATCCG	25	3.4181983E-5	46.0	39
CGTTAGG	25	3.4181983E-5	46.0	44
TAACACG	20	6.3127396E-4	46.0	41
ACGTACG	135	0.0	46.0	26
GACGAAT	20	6.3127396E-4	46.0	9
TATACGC	25	3.4181983E-5	46.0	35
CGATCGT	25	3.4181983E-5	46.0	26
TATAACG	25	3.4181983E-5	46.0	23
ACGAACG	25	3.4181983E-5	46.0	10
CGTATTG	150	0.0	44.466663	20
TTACGTA	140	0.0	44.357143	24
TAGGGAT	37155	0.0	43.418648	5
AGGGATC	9740	0.0	43.237164	6
TACGTAC	150	0.0	42.93333	25
CGCATAC	70	0.0	42.714287	23
TGACGTA	70	0.0	42.714287	25
ATAGGGA	100300	0.0	42.548855	4
>>END_MODULE