FastQCFastQC Report
Fri 17 Jun 2016
SRR1527133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168888
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT63640.29342225140256206No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGTGG36540.16847342970222529No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35240.162479574786711No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32110.14804821641320345No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31200.1438525179723434No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAGAC31000.1429303864468797No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC25500.11757176949662686No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT25260.11646521166607036No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTTTG24770.11420598942868421No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGCCA24220.11167012773365892No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC23430.10802770820807714No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC22830.10526131363168591No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22200.10235659932647515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA206.3126994E-446.034
AATTGCG301.8621722E-646.031
TCGTCTA206.3126994E-446.017
ATAACGA501.6370905E-1146.038
ATTATCG206.3126994E-446.039
ACGAACG301.8621722E-646.010
CGTACGT1050.043.80952527
ACGTACG1050.043.80952526
TAGGGAT359650.043.5314875
AGGGATC93600.043.0021366
TAGGGAC162750.042.721355
TAGGGGC203750.042.715095
ATAGGGA970800.042.7068374
AGGGATG92750.042.677096
TTCATAG2337250.042.558731
TAGGGAG289050.042.5545775
TCATAGG2329150.042.5349162
CATAGGG2293550.042.4941673
ACGGGTA650.042.4615430
ATAGGGG1065750.042.281594