##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2168888
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39768996831556	33.0	31.0	34.0	31.0	34.0
2	32.85861741131861	34.0	31.0	34.0	31.0	34.0
3	32.88457080310279	34.0	31.0	34.0	31.0	34.0
4	36.24765409739922	37.0	37.0	37.0	35.0	37.0
5	36.31076016834433	37.0	37.0	37.0	35.0	37.0
6	36.29313085784052	37.0	37.0	37.0	35.0	37.0
7	36.5018359638672	37.0	37.0	37.0	35.0	37.0
8	36.65208300290287	37.0	37.0	37.0	35.0	37.0
9	38.6704952030718	39.0	39.0	39.0	39.0	39.0
10	38.11884200567295	39.0	39.0	39.0	37.0	39.0
11	37.787181265238225	39.0	38.0	39.0	35.0	39.0
12	37.58662687976511	39.0	37.0	39.0	35.0	39.0
13	37.43957272113636	39.0	37.0	39.0	35.0	39.0
14	38.36206018936893	40.0	38.0	40.0	35.0	40.0
15	38.43520873369211	40.0	38.0	40.0	35.0	40.0
16	38.5046208010741	40.0	38.0	40.0	35.0	40.0
17	38.4803922563083	40.0	38.0	40.0	35.0	40.0
18	38.46003297542335	40.0	38.0	40.0	35.0	40.0
19	38.4721751422849	40.0	38.0	40.0	35.0	40.0
20	38.44713097218482	40.0	38.0	40.0	35.0	40.0
21	34.19605161723427	40.0	35.0	40.0	10.0	40.0
22	35.03410134594318	40.0	35.0	40.0	18.0	40.0
23	37.0928858474942	40.0	35.0	40.0	31.0	40.0
24	37.80825565912117	40.0	37.0	40.0	34.0	40.0
25	38.03669069126668	40.0	38.0	40.0	34.0	40.0
26	38.11550988340569	40.0	38.0	40.0	34.0	40.0
27	38.08230254397645	40.0	38.0	40.0	34.0	40.0
28	38.03647952314734	40.0	38.0	40.0	34.0	40.0
29	38.017208357462444	40.0	38.0	40.0	34.0	40.0
30	37.9098445839527	40.0	38.0	40.0	34.0	40.0
31	37.80714587383028	40.0	38.0	40.0	34.0	40.0
32	37.7798291105857	40.0	37.0	40.0	34.0	40.0
33	37.48929635831818	40.0	37.0	40.0	33.0	40.0
34	37.46868441339525	40.0	37.0	40.0	33.0	40.0
35	37.42700960123344	40.0	37.0	40.0	33.0	40.0
36	37.28300954221702	40.0	36.0	40.0	33.0	40.0
37	37.23336566941216	40.0	36.0	40.0	33.0	40.0
38	37.184218364433754	40.0	36.0	40.0	33.0	40.0
39	37.02901579058024	39.0	35.0	40.0	32.0	40.0
40	32.11650255799285	38.0	32.0	40.0	9.0	40.0
41	33.17098070531996	38.0	32.0	40.0	15.0	40.0
42	35.019264249698466	38.0	33.0	40.0	26.0	40.0
43	35.886369420643206	38.0	35.0	40.0	30.0	40.0
44	36.539563592034256	39.0	35.0	40.0	31.0	40.0
45	36.748207837380264	39.0	35.0	40.0	32.0	40.0
46	36.78123167263593	39.0	35.0	40.0	32.0	40.0
47	36.73384056714777	39.0	35.0	40.0	32.0	40.0
48	36.60401597500655	39.0	35.0	40.0	32.0	40.0
49	36.42768829003619	39.0	35.0	40.0	31.0	40.0
50	36.34726735543744	39.0	35.0	40.0	31.0	40.0
51	36.15738848663462	39.0	35.0	40.0	31.0	40.0
52	35.75662367074741	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	10.0
16	34.0
17	64.0
18	186.0
19	332.0
20	649.0
21	1075.0
22	1864.0
23	3064.0
24	4715.0
25	6544.0
26	9490.0
27	13217.0
28	18045.0
29	24313.0
30	32074.0
31	42838.0
32	59007.0
33	73396.0
34	124974.0
35	183634.0
36	288159.0
37	260373.0
38	593195.0
39	427630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.70846811822463	0.24265891092578318	0.013417013695497417	0.0354559571540808
8	99.57323753001538	0.3748464651010103	0.03282788230650915	0.019088122577099416
9	98.19935377022696	0.9862657730597431	0.25243350509569884	0.5619469516176031
10	46.226315051768466	42.32791181471796	4.1777168761134735	7.268056257400106
11	29.683044952067604	23.002248156659082	29.520749803585982	17.793957087687332
12	29.109110290618972	17.310529635462967	28.79383352206292	24.786526551855147
13	23.516059842647476	17.870355684572	32.25076629129766	26.36281818148286
14	26.91992394259178	19.3510683815854	30.147199855409777	23.581807820413044
15	25.06574797776556	20.49856885187248	29.268224085337742	25.167459085024213
16	27.724944764321624	22.47548052273792	27.366696666678962	22.432878046261497
17	28.998177868105685	20.486903888075364	24.394897292990695	26.12002095082826
18	25.19198778360155	21.093113152915226	27.683079993065572	26.03181907041765
19	25.612525865789287	21.16388674749457	27.280062409861642	25.943524976854498
20	24.324584764174084	23.12682812574923	28.07747564650641	24.47111146357027
21	31.304843772476953	20.170566668265028	27.53595390817783	20.988635651080184
22	25.82738251122234	21.118702302746843	27.988766593756804	25.06514859227401
23	24.003129714397424	22.30447123134067	29.742522435460018	23.949876618801895
24	25.011065578305562	23.075511506357174	26.475456547318256	25.437966368019005
25	24.15094739792926	26.04348403421477	25.50362213263202	24.301946435223947
26	26.32754665063387	24.63336050547562	25.782705238813623	23.256387605076885
27	26.30998004507379	23.46142354976375	25.340543172353758	24.8880532328087
28	28.50539078089786	22.15024473370686	24.88408806724921	24.460276418146073
29	27.66887916757343	22.738749073257818	24.610445537067843	24.98192622210091
30	26.69294126759888	23.13189984913928	25.85168067691831	24.323478206343527
31	27.690549258421825	24.196546801863445	24.368570437938704	23.744333501776023
32	26.859247688216264	24.024200419754273	24.831249930840134	24.28530196118933
33	27.867321871853225	23.006812707710125	24.840609565823595	24.285255854613055
34	25.35806367133757	25.02983095484875	26.304401149344734	23.307704224468946
35	25.912725783904012	25.195860736008495	26.813002792214256	22.07841068787323
36	26.2810712217505	25.591639586737536	25.336854646251904	22.79043454526006
37	28.109611930168825	26.36788990487291	23.24569087938151	22.276807285576755
38	25.67057404531723	26.41731615463777	25.013785866305682	22.898323933739317
39	26.2054103300862	25.509615987547534	24.555302071845112	23.72967161052115
40	26.236255629612966	29.933357554654737	22.64897956925392	21.18140724647838
41	25.091198807868366	25.63682403148526	25.26128596774015	24.01069119290623
42	23.709292503808406	24.3947128666856	26.616081604951475	25.279913024554517
43	24.534784645403544	25.295174301300943	26.15704453157563	24.012996521719888
44	24.361700558074	25.29913946686044	25.367377199744755	24.971782775320808
45	24.09598835901162	24.83074275850113	25.79284868559372	25.280420196893523
46	24.98049691823644	25.38323786198273	25.334134358251788	24.302130861529044
47	24.20922611033857	25.78819192139013	25.756193957456542	24.24638801081476
48	25.556275842736003	25.287658929368412	25.09161376705482	24.064451460840765
49	24.736593129751284	26.46236227965667	24.98252560759246	23.818518982999585
50	25.09230536569892	25.99765409739922	25.29835565506379	23.611684881838066
51	23.972053881989297	27.091163766870395	24.31683885936019	24.61994349178012
52	24.939738704810946	27.07129183249665	23.66701277336589	24.321956689326512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	8.5
2	17.0
3	11.5
4	6.0
5	60.5
6	115.0
7	919.0
8	1723.0
9	4353.0
10	6983.0
11	8249.5
12	9516.0
13	9363.5
14	9316.5
15	9422.0
16	9605.0
17	9788.0
18	9645.0
19	9502.0
20	10408.0
21	11314.0
22	13188.5
23	15063.0
24	15656.5
25	16250.0
26	22917.5
27	29585.0
28	30961.5
29	32338.0
30	37072.0
31	41806.0
32	42864.0
33	43922.0
34	48632.5
35	53343.0
36	59370.0
37	65397.0
38	66843.0
39	72865.5
40	77442.0
41	82000.5
42	86559.0
43	96613.0
44	106667.0
45	110252.0
46	113837.0
47	117803.5
48	121770.0
49	131039.5
50	140309.0
51	144294.0
52	148279.0
53	153459.5
54	158640.0
55	164507.0
56	170374.0
57	157966.5
58	145559.0
59	137281.0
60	129003.0
61	121887.0
62	114771.0
63	95272.0
64	62952.0
65	50131.0
66	41286.0
67	32441.0
68	28946.0
69	25451.0
70	23825.5
71	22200.0
72	14734.5
73	7269.0
74	6048.5
75	4828.0
76	3450.0
77	2072.0
78	1641.0
79	1210.0
80	904.5
81	599.0
82	329.5
83	60.0
84	53.5
85	47.0
86	25.5
87	4.0
88	3.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2168888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.786828809879719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.240590308066	6.084052137319928
2	9.888924039597125	1.7378456529968827
3	4.257444017267219	1.1222829524212095
4	2.2730908648076595	0.7989304119346539
5	1.2803178195768958	0.5624966751430326
6	0.812988674540536	0.42861553845352246
7	0.5548840374692985	0.34129697326043257
8	0.41317340896845	0.2904387210720153
9	0.29794104696101487	0.23561612775744942
>10	4.63136759456053	11.233358153100998
>50	3.3183521131180442	21.058917153555782
>100	2.890297026407278	44.68269026246531
>500	0.10608858056808618	6.138744941384212
>1k	0.033923674018864765	4.939806886564655
>5k	6.167940730702685E-4	0.3449074125699149
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTCATAGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCT	6364	0.29342225140256206	No Hit
TTCATAGGGATGTCATATTTATTCAGATTTATAAAGCTAGCAATGTCCGTGG	3654	0.16847342970222529	No Hit
TTCATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3524	0.162479574786711	No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3211	0.14804821641320345	No Hit
TTCATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3120	0.1438525179723434	No Hit
TTCATAGGGATATGTACTTTGAGTATAACATCAAGTTTTAACAAATAAAGAC	3100	0.1429303864468797	No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2550	0.11757176949662686	No Hit
TTCATAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2526	0.11646521166607036	No Hit
TTCATAGGGACTGTGGGAATTTTGCTTAGAACTCTTGAAGGAATATTCTTTG	2477	0.11420598942868421	No Hit
TTCATAGGGTTAGGCAAGAATTAGTGCTGTTGTATAAACAAAATACCAGCCA	2422	0.11167012773365892	No Hit
TTCATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2343	0.10802770820807714	No Hit
TTCATAGGGATATTTAGTGCTCTGTCTGCTGTCCCACTGTGTGGTCCAGTCC	2283	0.10526131363168591	No Hit
TTCATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2220	0.10235659932647515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.008483610034266408	0.0	0.0	0.0	0.0
7	0.008483610034266408	0.0	0.0	0.0	0.0
8	0.008714142915632342	0.0	0.0	0.0	0.0
9	0.009221315254637399	0.0	0.0	0.0	0.0
10	0.015076850441332149	0.0	0.0	0.0	0.0
11	0.02351435389932537	0.0	0.0	0.0	0.0
12	0.02950820881483968	0.0	0.0	0.0	0.0
13	0.03425718617097794	0.0	0.0	0.0	0.0
14	0.045829936815547874	0.0	0.0	0.0	0.0
15	0.05523567837527802	0.0	0.0	0.0	0.0
16	0.06763834739276532	0.0	0.0	0.0	0.0
17	0.0745082272574702	0.0	0.0	0.0	0.0
18	0.08142421369844824	0.0	0.0	0.0	0.0
19	0.08870905274961179	0.0	0.0	0.0	0.0
20	0.09530229315667753	0.0	0.0	0.0	0.0
21	0.10166500068237733	0.0	0.0	0.0	0.0
22	0.11116295539465385	0.0	0.0	0.0	0.0
23	0.11867832732718334	0.0	0.0	0.0	0.0
24	0.12674697817499106	0.0	0.0	0.0	0.0
25	0.13449288298888648	0.0	0.0	0.0	0.0
26	0.1418238286163232	0.0	0.0	0.0	0.0
27	0.1499385860404041	0.0	0.0	0.0	0.0
28	0.15897547498994877	0.0	0.0	0.0	0.0
29	0.1667213798038442	0.0	0.0	0.0	0.0
30	0.17492835038047147	0.0	0.0	0.0	0.0
31	0.18350417356728424	0.0	0.0	0.0	0.0
32	0.19318655458465353	0.0	0.0	0.0	4.6106576273186995E-5
33	0.19991811472053883	0.0	0.0	0.0	4.6106576273186995E-5
34	0.20789455241580018	0.0	0.0	0.0	4.6106576273186995E-5
35	0.21789967946708175	0.0	0.0	0.0	4.6106576273186995E-5
36	0.22951853668792488	0.0	0.0	0.0	4.6106576273186995E-5
37	0.23698780204418116	0.0	0.0	0.0	4.6106576273186995E-5
38	0.24699292909546275	0.0	0.0	0.0	4.6106576273186995E-5
39	0.254462194451719	0.0	0.0	0.0	4.6106576273186995E-5
40	0.2645134280792738	0.0	0.0	0.0	4.6106576273186995E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	20	6.3126994E-4	46.0	34
AATTGCG	30	1.8621722E-6	46.0	31
TCGTCTA	20	6.3126994E-4	46.0	17
ATAACGA	50	1.6370905E-11	46.0	38
ATTATCG	20	6.3126994E-4	46.0	39
ACGAACG	30	1.8621722E-6	46.0	10
CGTACGT	105	0.0	43.809525	27
ACGTACG	105	0.0	43.809525	26
TAGGGAT	35965	0.0	43.531487	5
AGGGATC	9360	0.0	43.002136	6
TAGGGAC	16275	0.0	42.72135	5
TAGGGGC	20375	0.0	42.71509	5
ATAGGGA	97080	0.0	42.706837	4
AGGGATG	9275	0.0	42.67709	6
TTCATAG	233725	0.0	42.55873	1
TAGGGAG	28905	0.0	42.554577	5
TCATAGG	232915	0.0	42.534916	2
CATAGGG	229355	0.0	42.494167	3
ACGGGTA	65	0.0	42.46154	30
ATAGGGG	106575	0.0	42.28159	4
>>END_MODULE