FastQCFastQC Report
Fri 17 Jun 2016
SRR1527132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762019
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT76100.9986627630019723No Hit
CTCCATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC51740.6789856945824185No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGA51440.675048784872818No Hit
CTCCATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA48190.6323989296854803No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT44830.5883055409379556No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41290.5418500063646707No Hit
CTCCATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG38310.5027433699159732No Hit
CTCCATGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG34970.4589124418157553No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC27960.36691998493475886No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG24570.32243290521627416No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG22970.30143605343173857No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21420.28109535326546975No Hit
CTCCATGGGAATTAACTTTGGGCTCCATAGTTTGTTCCTAATAGAATTTT21270.27912689841066957No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20990.2754524493483758No Hit
CTCCATGGGGGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGT19990.2623294169830411No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA19920.2614108047174677No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17040.2236164715053037No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC16720.21941710114839658No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG16440.21574265208610283No Hit
CTCCATGGGTACTGCCCAGATCTTGGCCTCCTCAAAGAGCCTTTTTGCTT16420.21548019143879615No Hit
CTCCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC15530.20380069263364825No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGG14250.1870032112060198No Hit
CTCCATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG14000.18372245311468613No Hit
CTCCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA13370.17545494272452525No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG12660.1661375897451376No Hit
CTCCATGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA12550.16469405618495075No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC12110.15891992194420348No Hit
CTCCATGGGGATGCCACCCATCACCAACCACCTGCCTTCTCTTTGATTTC11920.1564265457947899No Hit
CTCCATGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT11820.1551142425582564No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11650.15288332705614952No Hit
CTCCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG10740.14094136760369494No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT10590.1389729127488947No Hit
CTCCATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCT10070.13214893591892066No Hit
CTCCATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC9730.12768710491470686No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG9670.12689972297278676No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG9550.12532495908894659No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT9460.12414388617606646No Hit
CTCCATGGGGTCCCCCAGGAGGTGAGGTTGATTTCCATCTGCCAGAACAG9460.12414388617606646No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT9300.12204420099761293No Hit
CTCCATGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC9300.12204420099761293No Hit
CTCCATGGGGATCATTGAAACCATGAACAGAGCCCCGTGTGTGTCCGTGG9240.12125681905569283No Hit
CTCCATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA9130.11981328549550603No Hit
CTCCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT9070.11902590355358593No Hit
CTCCATGGGATGTTTATTGGGAGAGCTTGATTGTAGAGGCGAAAAGACTT9030.11850098225897254No Hit
CTCCATGGGGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCCCT8960.1175823699933991No Hit
CTCCATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8850.11613883643321231No Hit
CTCCATGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAG8750.11482653319667882No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA8750.11482653319667882No Hit
CTCCATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGAC8610.11298930866553197No Hit
CTCCATGGGGCTTCTCGGTCACAGGATTCCATTTAACCACAACTCGCCAC8580.11259561769457191No Hit
CTCCATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT8560.11233315704726522No Hit
CTCCATGGGGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGT8490.11141454478169178No Hit
CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA8450.11088962348707841No Hit
CTCCATGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA8410.11036470219246501No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC8210.10774009571939808No Hit
CTCCATGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC8170.10721517442478469No Hit
CTCCATGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGA7980.10472179827537109No Hit
CTCCATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC7960.10445933762806439No Hit
CTCCATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG7830.10275334342057087No Hit
CTCCATGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG7670.10065365824211733No Hit
CTCCATGGGGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGCGCCGTC7630.10012873694750393No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG254.4414224E-544.042
ATTTACG254.4414224E-544.020
GAGGATA254.4414224E-544.010
AACGTAT254.4414224E-544.017
TTAGGGT254.4414224E-544.038
AAGTACG207.854809E-444.021
AACCGCA207.854809E-444.041
AATCCGT254.4414224E-544.030
GATACAC207.854809E-444.020
ACAAATC351.4452235E-744.024
GATCGTA207.854809E-444.041
CATATAG408.305506E-944.011
CGTATGG302.526589E-644.019
ATAGGTT502.7284841E-1144.05
ATTAGAC502.7284841E-1144.010
CTACTAG207.854809E-444.022
CTCAACC302.526589E-644.044
CAACGTA254.4414224E-544.016
GGTCGAC207.854809E-444.09
TTTACGG207.854809E-444.022
Produced by FastQC (version 0.11.5)