FastQCFastQC Report
Fri 17 Jun 2016
SRR1527131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826386
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT71180.8613408261998631No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG46990.5686204751774594No Hit
CTCCATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC46870.5671683692608539No Hit
CTCCATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA46480.5624490250318858No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45420.5496220894352034No Hit
CTCCATGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT40250.48706052619478063No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC38840.4699982816746654No Hit
CTCCATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA36780.44507046343960327No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA29280.35431384365175594No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23420.28340267139085124No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC21610.2615000738153841No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC21010.2542395442323563No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20530.24843112056593405No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG19890.2406865556773711No Hit
CTCCATGGGGGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCT19700.23838738797607897No Hit
CTCCATGGGAATTAACTTTGGGCTCCATAGTTTGTTCCTAATAGAATTTTCT19290.23342602609434335No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16030.1939771486932257No Hit
CTCCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG16020.1938561398668419No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC16020.1938561398668419No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG15890.19228302512385254No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG15270.18478047788805715No Hit
CTCCATGGGTACTGCCCAGATCTTGGCCTCCTCAAAGAGCCTTTTTGCTTTA13370.16178880087513584No Hit
CTCCATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT12210.14775177701461545No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT12020.14545260931332332No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11880.14375848574395014No Hit
CTCCATGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA11840.14327445043841497No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11760.14230637982734462No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10640.1287533912723594No Hit
CTCCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG10530.12742229418213766No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT10410.12597018826553208No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA10050.12161387051571541No Hit
CTCCATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT9990.12088781755741265No Hit
CTCCATGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT9740.11786259689781772No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC9360.11326426149523346No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA9310.11265921736331448No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9230.1116911467522441No Hit
CTCCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG9220.11157013792586032No Hit
CTCCATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT9180.11108610262032513No Hit
CTCCATGGGATGTTTATTGGGAGAGCTTGATTGTAGAGGCGAAAAGACTTCG9150.11072307614117373No Hit
CTCCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8950.10830289961349782No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG8890.10757684665519503No Hit
CTCCATGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT8800.10648776721774086No Hit
CTCCATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA8740.10576171425943807No Hit
CTCCATGGGGCTTCTCGGTCACAGGATTCCATTTAACCACAACTCGCCACCT8550.10346254655814593No Hit
CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG8450.10225245829430799No Hit
CTCCATGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC8420.10188943181515661No Hit
CTCCATGGGGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCCCTAC8410.1017684229887728No Hit
CTCCATGGGGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCG8390.10152640533600521No Hit
CTCCATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC8310.10055833472493485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATC206.310418E-446.00000436
AGCGTTA206.310418E-446.00000428
CTTCGCA351.019107E-746.00000445
CGGAACA206.310418E-446.00000424
TGATAGC206.310418E-446.00000418
CCTTACG206.310418E-446.00000412
CGAACTT206.310418E-446.00000423
CAAACCG206.310418E-446.00000413
CGAACCC351.019107E-746.00000445
CATATAG206.310418E-446.00000411
AGATACC206.310418E-446.00000420
CATTGAT405.6079443E-946.00000432
TCGGGTA206.310418E-446.00000425
GCACGAA206.310418E-446.00000419
TTCGTTA206.310418E-446.00000439
CAATTAA206.310418E-446.00000431
CCTGTCG351.019107E-746.00000428
TAACGCG405.6079443E-946.00000434
GCGTTAT351.019107E-746.00000441
TATGCAG351.019107E-746.00000437
Produced by FastQC (version 0.11.5)