FastQCFastQC Report
Fri 17 Jun 2016
SRR1527130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800038
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT69330.8665838372677297No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG46720.5839722613175874No Hit
CTCCATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA45660.5707228906626935No Hit
CTCCATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC44620.5577235081333637No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44150.5518487871826089No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC36830.4603531332261717No Hit
CTCCATGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT36590.4573532757194033No Hit
CTCCATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA35810.4476037388224059No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA29670.37085738427424697No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23590.2948609941027801No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22790.2848614690802187No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC21080.26348748434449365No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG20720.258987698084341No Hit
CTCCATGGGAATTAACTTTGGGCTCCATAGTTTGTTCCTAATAGAATTTTCT20470.2558628465147905No Hit
CTCCATGGGGGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCT19900.24873818493621552No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG18290.22861414082831066No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG16100.20124044107904873No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15760.19699064294446014No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT15450.19311582699821758No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG15290.19111592199370528No Hit
CTCCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG14830.1853661951057325No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT12850.16061737067489293No Hit
CTCCATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT12630.15786750129368857No Hit
CTCCATGGGTACTGCCCAGATCTTGGCCTCCTCAAAGAGCCTTTTTGCTTTA12240.15299273284518985No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11990.1498678812756394No Hit
CTCCATGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA11590.1448681187643587No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11280.14099330281811615No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10300.1287438846654784No Hit
CTCCATGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT9940.12424409840532576No Hit
CTCCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9850.1231191518402876No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA9330.11661946057562265No Hit
CTCCATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT9090.11361960306885423No Hit
CTCCATGGGGTCCCCCAGGAGGTGAGGTTGATTTCCATCTGCCAGAACAGGG9080.11349460900607221No Hit
CTCCATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT9010.1126196505665981No Hit
CTCCATGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT8900.11124471587599588No Hit
CTCCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8850.1106197455620858No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC8800.1099947752481757No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8770.10961979305982965No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG8710.10886982868313755No Hit
CTCCATGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC8660.10824485836922745No Hit
CTCCATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC8650.10811986430644543No Hit
CTCCATGGGATGTTTATTGGGAGAGCTTGATTGTAGAGGCGAAAAGACTTCG8530.10661993555306122No Hit
CTCCATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA8430.10536999492524106No Hit
CTCCATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8400.104995012736895No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC8310.10387006617185685No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8240.10299510773238271No Hit
CTCCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG8160.10199515523012657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAC206.310297E-446.00000426
ACTATAC206.310297E-446.00000433
CCCCTAA206.310297E-446.00000434
ACACGTC206.310297E-446.00000441
CACGGTA206.310297E-446.00000413
CTATACC206.310297E-446.00000434
CGCATAT551.8189894E-1246.00000428
ATTACAT206.310297E-446.00000410
TTCACGC206.310297E-446.00000437
CGCGATT206.310297E-446.00000418
CGATTGC206.310297E-446.00000420
CGATTAA206.310297E-446.00000430
CCTAATT206.310297E-446.00000437
CCTAATA2200.046.00000437
TCGATTG800.046.00000425
TCGATTA206.310297E-446.00000429
CACGAAC206.310297E-446.00000432
TACGGCT405.6079443E-946.00000424
TAACGAT206.310297E-446.00000422
TCGATAC206.310297E-446.00000424
Produced by FastQC (version 0.11.5)