##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800038
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.480609671040625	33.0	31.0	34.0	31.0	34.0
2	32.712044927865925	34.0	31.0	34.0	31.0	34.0
3	32.96198180586422	34.0	31.0	34.0	31.0	34.0
4	36.46268802231894	37.0	37.0	37.0	35.0	37.0
5	36.018306630435056	37.0	35.0	37.0	35.0	37.0
6	36.264321194743246	37.0	37.0	37.0	35.0	37.0
7	36.54620905506989	37.0	37.0	37.0	35.0	37.0
8	36.64676802851864	37.0	37.0	37.0	35.0	37.0
9	38.64696676907847	39.0	39.0	39.0	39.0	39.0
10	38.13884715476015	39.0	39.0	39.0	37.0	39.0
11	37.82574702701622	39.0	38.0	39.0	35.0	39.0
12	37.38521795214727	39.0	37.0	39.0	35.0	39.0
13	37.197376874598454	39.0	37.0	39.0	34.0	39.0
14	37.99608518595367	40.0	38.0	40.0	34.0	40.0
15	38.13501608673588	40.0	38.0	40.0	34.0	40.0
16	38.11669195713203	40.0	38.0	40.0	34.0	40.0
17	38.131245015861744	40.0	38.0	40.0	34.0	40.0
18	38.158379976951096	40.0	38.0	40.0	34.0	40.0
19	37.98483822018454	40.0	38.0	40.0	34.0	40.0
20	37.971778840505074	40.0	38.0	40.0	34.0	40.0
21	33.77656061337086	40.0	34.0	40.0	10.0	40.0
22	34.57309777785555	40.0	34.0	40.0	17.0	40.0
23	36.570434154377665	39.0	34.0	40.0	30.0	40.0
24	37.3468135263575	39.0	36.0	40.0	33.0	40.0
25	37.59623667875776	39.0	37.0	40.0	33.0	40.0
26	37.64567683035056	40.0	37.0	40.0	33.0	40.0
27	37.61454205925219	40.0	37.0	40.0	33.0	40.0
28	37.4862694022034	40.0	36.0	40.0	33.0	40.0
29	37.532215969741436	40.0	36.0	40.0	33.0	40.0
30	37.42114124579083	40.0	36.0	40.0	33.0	40.0
31	37.24426214754799	40.0	36.0	40.0	33.0	40.0
32	37.09373179773961	40.0	35.0	40.0	32.0	40.0
33	36.81527127461445	39.0	35.0	40.0	31.0	40.0
34	36.703432836940244	39.0	35.0	40.0	31.0	40.0
35	36.6562463282994	39.0	35.0	40.0	31.0	40.0
36	36.55791724893068	39.0	35.0	40.0	31.0	40.0
37	36.28167412047928	39.0	35.0	40.0	30.0	40.0
38	36.28968998972549	39.0	35.0	40.0	30.0	40.0
39	36.179090243213444	38.0	35.0	40.0	30.0	40.0
40	31.248723185648682	37.0	30.0	40.0	9.0	40.0
41	32.13218247133261	37.0	30.0	40.0	13.0	40.0
42	33.9461388084066	36.0	31.0	40.0	23.0	40.0
43	34.744868368752485	37.0	33.0	40.0	27.0	40.0
44	35.526776228129165	38.0	34.0	40.0	29.0	40.0
45	35.80153567705534	38.0	35.0	40.0	30.0	40.0
46	35.80569797934598	38.0	35.0	40.0	30.0	40.0
47	35.73290018724111	37.0	35.0	40.0	30.0	40.0
48	35.609662291041175	37.0	35.0	40.0	30.0	40.0
49	35.493957787005115	37.0	35.0	40.0	30.0	40.0
50	35.32114599556521	37.0	34.0	40.0	29.0	40.0
51	35.12010804486787	36.0	34.0	40.0	29.0	40.0
52	34.708045117856905	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	6.0
16	11.0
17	45.0
18	92.0
19	163.0
20	341.0
21	680.0
22	1234.0
23	1838.0
24	2719.0
25	3927.0
26	5262.0
27	7254.0
28	9731.0
29	12124.0
30	15484.0
31	20491.0
32	29021.0
33	33967.0
34	58678.0
35	82020.0
36	97025.0
37	106644.0
38	195347.0
39	115930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.4597756606561	0.3262345038610666	0.051997530117319425	0.16199230536549514
8	99.420277536817	0.2961109347306003	0.21548976423619876	0.06812176421619973
9	98.4103255095383	1.1394458763208748	0.1988655538861904	0.2513630602546379
10	62.74601956407071	25.863521482729567	3.8460673118026896	7.544391641397033
11	32.95630957529517	28.017794154777647	17.484669478199784	21.541226791727393
12	30.224064356943046	15.491264164952165	27.889925228551643	26.394746249553148
13	21.361735317572414	15.99511523202648	30.889407753131724	31.753741697269376
14	20.598146588037068	18.91847637237231	32.89793734797597	27.58543969161465
15	23.799494524010107	16.070111669695688	30.85503438586667	29.275359420427527
16	29.613843342441232	16.662958509470798	27.41419782560328	26.309000322484682
17	33.671400608471096	20.595271724593083	20.367407548141465	25.365920118794357
18	33.10292761093848	18.08889077768806	24.033858391726394	24.77432321964707
19	32.177471570100415	18.015644256897797	24.95606458693212	24.85081958606966
20	30.80716165982116	22.631550001374933	22.11144970613896	24.44983863266495
21	36.05603733822643	16.613085878420776	25.641282039103142	21.68959474424965
22	33.009557046040314	16.567088063316994	24.87669335706554	25.546661533577154
23	31.378759508923327	21.326361997805105	22.42918461373085	24.86569387954072
24	29.558720960754364	22.492181621372982	21.609598544069154	26.339498873803496
25	27.165959616918194	21.040250588097066	21.325237051240066	30.46855274374467
26	28.998747559490923	22.500806211704944	21.79508973323767	26.705356495566463
27	27.190083471035127	20.020924006109713	22.544304145553088	30.244688377302076
28	27.58681462630525	24.271222116949446	21.278864253947937	26.86309900279737
29	31.46150557848502	20.754639154640156	20.42490481702119	27.35895044985363
30	27.69455950842335	20.92213119876806	23.349515897994845	28.033793394813745
31	32.32283966511591	19.16108984823221	21.65184653728948	26.864223949362405
32	33.779020496526414	19.516572962784267	23.022656423819868	23.68175011686945
33	37.03136601011452	18.003269844682375	21.577225081808614	23.38813906339449
34	32.27759181438882	19.64056707306403	24.050732590201964	24.03110852234519
35	28.085915918993848	26.740854809396552	24.567333051680045	20.605896219929555
36	34.23437386724131	24.032108474847444	18.854354418165137	22.879163239746113
37	31.193518307880375	25.06318449873631	21.094498011344463	22.648799182038854
38	32.068226759228935	25.41341786265152	19.249460650619096	23.268894727500445
39	31.270139668365754	22.09182563828218	19.635942292741095	27.002092400610973
40	29.102117649411653	26.618360627870175	21.49022921411233	22.78929250860584
41	24.75157430022074	22.82516580462428	20.932505705978965	31.490754189176013
42	25.15543011706944	20.256037838202683	24.26584737224982	30.322684672478058
43	24.774698201835413	19.42132748694437	25.95014236823751	29.853831942982705
44	26.89284758973949	20.071546601536426	23.68912476657359	29.346481042150497
45	29.001247440746564	21.35398568567993	21.276739354880643	28.368027518692863
46	28.710011274464463	19.387454095930444	24.926690982178346	26.975843647426746
47	29.190988428049668	19.514698051842537	24.0678567768031	27.226456743304695
48	30.3613078378777	18.713736097535367	23.843742422234943	27.081213642351987
49	29.57172034328369	20.402155897594866	24.146853024481338	25.8792707346401
50	27.067339301383186	21.836337773955737	24.873943487684336	26.222379436976745
51	25.62890762688772	23.189523497633864	22.81466630335059	28.366902572127824
52	28.324654578907506	21.50122869163715	24.733950137368474	25.440166592086875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	5.5
10	11.0
11	73.5
12	136.0
13	128.0
14	231.5
15	343.0
16	353.0
17	363.0
18	367.5
19	372.0
20	721.5
21	1071.0
22	937.5
23	804.0
24	1336.0
25	1868.0
26	1999.5
27	2131.0
28	2380.0
29	2629.0
30	2675.0
31	2721.0
32	3236.5
33	3752.0
34	5185.5
35	6619.0
36	8167.5
37	9716.0
38	8729.5
39	8411.0
40	9079.0
41	11240.5
42	13402.0
43	13258.5
44	13115.0
45	17085.0
46	21055.0
47	23964.5
48	26874.0
49	36510.5
50	46147.0
51	42016.5
52	37886.0
53	39387.0
54	40888.0
55	48990.5
56	57093.0
57	63323.5
58	69554.0
59	76067.5
60	82581.0
61	77561.5
62	72542.0
63	74730.0
64	69705.0
65	62492.0
66	52021.5
67	41551.0
68	33765.0
69	25979.0
70	23895.0
71	21811.0
72	19333.5
73	16856.0
74	15268.0
75	13680.0
76	10083.5
77	6487.0
78	4356.0
79	2225.0
80	1652.0
81	1079.0
82	682.0
83	285.0
84	157.5
85	30.0
86	28.0
87	26.0
88	13.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	800038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.462525676865887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.43915135099526	10.042655093935455
2	11.53714792786141	3.337125962889914
3	5.2960008920838915	2.2978064665940376
4	2.8388261777582775	1.6422638595195251
5	1.8137800606833596	1.3115920349910228
6	1.1790839457572335	1.02315191044166
7	0.7650808359949703	0.7745500864818694
8	0.5731604125898239	0.6631477747234698
9	0.42341511881477745	0.5511286825048746
>10	3.086109841861197	10.257611075481442
>50	1.493140135732826	15.743629447577517
>100	1.4664615511750638	37.18797844008665
>500	0.06454496263974752	6.498038644639753
>1k	0.02323618655030911	7.806408669012514
>5k	8.605995018633004E-4	0.8629118511202768
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	6933	0.8665838372677297	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGAAG	4672	0.5839722613175874	No Hit
CTCCATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	4566	0.5707228906626935	No Hit
CTCCATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	4462	0.5577235081333637	No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4415	0.5518487871826089	No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3683	0.4603531332261717	No Hit
CTCCATGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	3659	0.4573532757194033	No Hit
CTCCATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	3581	0.4476037388224059	No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2967	0.37085738427424697	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2359	0.2948609941027801	No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2279	0.2848614690802187	No Hit
CTCCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2108	0.26348748434449365	No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2072	0.258987698084341	No Hit
CTCCATGGGAATTAACTTTGGGCTCCATAGTTTGTTCCTAATAGAATTTTCT	2047	0.2558628465147905	No Hit
CTCCATGGGGGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCT	1990	0.24873818493621552	No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1829	0.22861414082831066	No Hit
CTCCATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1610	0.20124044107904873	No Hit
CTCCATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1576	0.19699064294446014	No Hit
CTCCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1545	0.19311582699821758	No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCATGGGGAG	1529	0.19111592199370528	No Hit
CTCCATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1483	0.1853661951057325	No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1285	0.16061737067489293	No Hit
CTCCATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	1263	0.15786750129368857	No Hit
CTCCATGGGTACTGCCCAGATCTTGGCCTCCTCAAAGAGCCTTTTTGCTTTA	1224	0.15299273284518985	No Hit
CTCCATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1199	0.1498678812756394	No Hit
CTCCATGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA	1159	0.1448681187643587	No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1128	0.14099330281811615	No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1030	0.1287438846654784	No Hit
CTCCATGGGGTCTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	994	0.12424409840532576	No Hit
CTCCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	985	0.1231191518402876	No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	933	0.11661946057562265	No Hit
CTCCATGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	909	0.11361960306885423	No Hit
CTCCATGGGGTCCCCCAGGAGGTGAGGTTGATTTCCATCTGCCAGAACAGGG	908	0.11349460900607221	No Hit
CTCCATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT	901	0.1126196505665981	No Hit
CTCCATGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	890	0.11124471587599588	No Hit
CTCCATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	885	0.1106197455620858	No Hit
CTCCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	880	0.1099947752481757	No Hit
CTCCATGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	877	0.10961979305982965	No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	871	0.10886982868313755	No Hit
CTCCATGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC	866	0.10824485836922745	No Hit
CTCCATGGGGCTCGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGC	865	0.10811986430644543	No Hit
CTCCATGGGATGTTTATTGGGAGAGCTTGATTGTAGAGGCGAAAAGACTTCG	853	0.10661993555306122	No Hit
CTCCATGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	843	0.10536999492524106	No Hit
CTCCATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	840	0.104995012736895	No Hit
CTCCATGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC	831	0.10387006617185685	No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	824	0.10299510773238271	No Hit
CTCCATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	816	0.10199515523012657	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.499881255640357E-4	0.0	0.0	0.0	0.0
8	2.499881255640357E-4	0.0	0.0	0.0	0.0
9	0.0031248515695504464	0.0	0.0	0.0	0.0
10	0.011999430027073713	0.0	0.0	0.0	0.0
11	0.02974858694212025	0.0	0.0	0.0	0.0
12	0.03424837320227289	0.0	0.0	0.0	0.0
13	0.03787320102295141	0.0	0.0	0.0	0.0
14	0.05962216794702252	0.0	0.0	0.0	0.0
15	0.06562188296055937	0.0	0.0	0.0	0.0
16	0.07474644954364668	0.0	0.0	0.0	0.0
17	0.08012119424327345	0.0	0.0	0.0	0.0
18	0.083996010189516	0.0	0.0	0.0	0.0
19	0.08999572520305285	0.0	0.0	0.0	0.0
20	0.09699539271884586	0.0	0.0	0.0	0.0
21	0.1022451433556906	0.0	0.0	0.0	0.0
22	0.10799487024366343	0.0	0.0	0.0	0.0
23	0.11711943682675073	0.0	0.0	0.0	0.0
24	0.12549403903314593	0.0	0.0	0.0	0.0
25	0.13036880748164462	0.0	0.0	0.0	0.0
26	0.1407433146925521	0.0	0.0	0.0	0.0
27	0.14961789315007537	0.0	0.0	0.0	0.0
28	0.2206145208102615	0.0	0.0	0.0	0.0
29	0.23011406958169486	0.0	0.0	0.0	0.0
30	0.24698826805726729	0.0	0.0	0.0	0.0
31	0.2548628940125344	0.0	0.0	0.0	0.0
32	0.2619875555911094	0.0	0.0	0.0	0.0
33	0.26761228841630025	0.0	0.0	0.0	0.0
34	0.2752369262460033	0.0	0.0	0.0	0.0
35	0.33610903482084603	0.0	0.0	0.0	1.2499406278201786E-4
36	0.34810846484791974	0.0	0.0	0.0	1.2499406278201786E-4
37	0.35460815611258467	0.0	0.0	0.0	1.2499406278201786E-4
38	0.36010789487499345	0.0	0.0	0.0	1.2499406278201786E-4
39	0.3669825683280044	0.0	0.0	0.0	1.2499406278201786E-4
40	0.37698209335056587	0.0	0.0	0.0	1.2499406278201786E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAC	20	6.310297E-4	46.000004	26
ACTATAC	20	6.310297E-4	46.000004	33
CCCCTAA	20	6.310297E-4	46.000004	34
ACACGTC	20	6.310297E-4	46.000004	41
CACGGTA	20	6.310297E-4	46.000004	13
CTATACC	20	6.310297E-4	46.000004	34
CGCATAT	55	1.8189894E-12	46.000004	28
ATTACAT	20	6.310297E-4	46.000004	10
TTCACGC	20	6.310297E-4	46.000004	37
CGCGATT	20	6.310297E-4	46.000004	18
CGATTGC	20	6.310297E-4	46.000004	20
CGATTAA	20	6.310297E-4	46.000004	30
CCTAATT	20	6.310297E-4	46.000004	37
CCTAATA	220	0.0	46.000004	37
TCGATTG	80	0.0	46.000004	25
TCGATTA	20	6.310297E-4	46.000004	29
CACGAAC	20	6.310297E-4	46.000004	32
TACGGCT	40	5.6079443E-9	46.000004	24
TAACGAT	20	6.310297E-4	46.000004	22
TCGATAC	20	6.310297E-4	46.000004	24
>>END_MODULE