FastQCFastQC Report
Fri 17 Jun 2016
SRR1527129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415291
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT123370.8716935245119202No Hit
TTAACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA99880.7057205903238274No Hit
TTAACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC83840.5923870073363005No Hit
TTAACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG67240.47509664090282494No Hit
TTAACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA62580.4421705500847529No Hit
TTAACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA57960.40952708665567716No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC45190.3192982927185999No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG44030.3111020984377065No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA36670.25909865886238237No Hit
TTAACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC34720.24532057364881144No Hit
TTAACTGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAAT29060.20532879810583124No Hit
TTAACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA28660.20250252421586795No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG28420.20080675988189No Hit
TTAACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG27480.19416501624047633No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG26610.18801787052980623No Hit
TTAACTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA25680.18144678373564163No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT25670.18137612688839255No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT23090.1631466602981295No Hit
TTAACTGGGTCTTTTTGTTCGGGGCTTGTTTTATTCAACTCCGAATTGAA21780.15389061330849982No Hit
TTAACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT21050.14873266345931685No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA20820.14710755597258796No Hit
TTAACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA20300.14343339991563572No Hit
TTAACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC19950.14096041026191786No Hit
TTAACTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG19390.1370036268159693No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC19020.13438932346775326No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18850.1331881570645189No Hit
TTAACTGGGTCTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGC18490.13064451056355195No Hit
TTAACTGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA17780.12562787440886716No Hit
TTAACTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA17320.12237765943540939No Hit
TTAACTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA17310.12230700258816032No Hit
TTAACTGGGCTGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACC17280.12209503204641306No Hit
TTAACTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC17060.12054058140693327No Hit
TTAACTGGGGATGTTCTCAAGTGATTTAGCTGCCATTCTGCCATTTTGAG16640.11757299382247186No Hit
TTAACTGGGACACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCC15960.1127683282095343No Hit
TTAACTGGGATCTCACCTAGATCTTTCTCCCCCAGGTCATCTCAGCTCAG15660.11064862279206183No Hit
TTAACTGGGAGGCCACCAACTTCTTTTCCTAAAGAATTATTTTTCATTTG15230.10761037836035134No Hit
TTAACTGGGGATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGT14420.10188717373317573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAT551.8189894E-1244.00000429
AATCCGA302.5281352E-644.029
CGTATTA408.314601E-944.025
CGAAATC600.044.025
TTAGCTA600.044.025
ACGCATC302.5281352E-644.038
CTTCATA302.5281352E-644.031
TAGGTCG207.8571006E-444.025
TCTTATA302.5281352E-644.028
ATTCGCG254.4433626E-544.034
ATAGAGT302.5281352E-644.037
CGATTAG254.4433626E-544.044
CGCGAAT302.5281352E-644.016
ATGTACG254.4433626E-544.040
ACCTATA408.314601E-944.044
GTAGCGT207.8571006E-444.09
CCCGTAA207.8571006E-444.027
CTTATAT254.4433626E-544.039
CTAGTAC502.7284841E-1144.040
TACGATT254.4433626E-544.032
Produced by FastQC (version 0.11.5)