FastQCFastQC Report
Fri 17 Jun 2016
SRR1527128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512208
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT107590.7114761990414017No Hit
TTAACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG97210.6428348481161322No Hit
TTAACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC76540.5061473024874885No Hit
TTAACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA64670.42765280966639513No Hit
TTAACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG58630.38771121433030376No Hit
TTAACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA54140.35801953170463324No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA47210.31219250261868736No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC41680.27562345920667No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33800.22351422555627268No Hit
TTAACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG33520.22166262842148698No Hit
TTAACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA28500.18846613693354355No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26660.17629849861923755No Hit
TTAACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA26300.17391787373165596No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25550.16895823854919428No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG25210.16670987059981165No Hit
TTAACTGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA24700.16333731867573775No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC23970.15850994043147504No Hit
TTAACTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG23760.15712124258038576No Hit
TTAACTGGGTCTTTTTGTTCGGGGCTTGTTTTATTCAACTCCGAATTGAATG20900.13820850041793192No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT20810.13761334419603652No Hit
TTAACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC20800.137547215726937No Hit
TTAACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC18480.1222054108958556No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18010.1190973728481796No Hit
TTAACTGGGTCTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTA17360.11479902235671284No Hit
TTAACTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT17000.11241839746913122No Hit
TTAACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA16960.11215388359273329No Hit
TTAACTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16760.11083131421074349No Hit
TTAACTGGGCTGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA16670.1102361579888481No Hit
TTAACTGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT16350.10812004697766445No Hit
TTAACTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA15710.10388782495529716No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC15490.1024329986351084No Hit
TTAACTGGGATCTCACCTAGATCTTTCTCCCCCAGGTCATCTCAGCTCAGTC15280.10104430078401913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGT405.6134013E-946.027
GTCGATA206.31209E-446.029
CGACGTA253.4176708E-546.038
CGCATTC253.4176708E-546.037
ATTCGTT253.4176708E-546.041
CGCGAAT206.31209E-446.016
TTATCCG206.31209E-446.014
ACCTATA301.8617957E-646.044
GCCGATA206.31209E-446.011
ATATCGT206.31209E-446.037
ACGATAC206.31209E-446.045
TCTAACG301.8617957E-646.013
AACGATA206.31209E-446.044
AATTCGG206.31209E-446.033
AATTCGC301.8617957E-646.016
CGTACAC253.4176708E-546.010
TATAGGG253.4176708E-546.034
GTATACC253.4176708E-546.031
TTACGAT206.31209E-446.014
ACGTATA253.4176708E-546.016
Produced by FastQC (version 0.11.5)