##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1512208
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39779778972205	33.0	31.0	34.0	31.0	34.0
2	32.864361251891275	34.0	31.0	34.0	31.0	34.0
3	32.71567800196798	34.0	31.0	34.0	31.0	34.0
4	36.47091405415128	37.0	37.0	37.0	35.0	37.0
5	36.39430157756076	37.0	37.0	37.0	35.0	37.0
6	36.393635002592234	37.0	37.0	37.0	35.0	37.0
7	36.51274427856485	37.0	37.0	37.0	35.0	37.0
8	36.62325817612392	37.0	37.0	37.0	35.0	37.0
9	38.71905716673897	39.0	39.0	39.0	39.0	39.0
10	38.173055558494596	39.0	39.0	39.0	37.0	39.0
11	37.87610500671865	39.0	38.0	39.0	35.0	39.0
12	37.60738271454721	39.0	37.0	39.0	35.0	39.0
13	37.54526890480675	39.0	37.0	39.0	35.0	39.0
14	38.39849081607821	40.0	38.0	40.0	35.0	40.0
15	38.291360712282966	40.0	38.0	40.0	35.0	40.0
16	33.209151122067865	40.0	35.0	40.0	3.0	40.0
17	34.97410144636188	40.0	35.0	40.0	17.0	40.0
18	37.05021068530255	40.0	35.0	40.0	32.0	40.0
19	37.937103229185404	40.0	37.0	40.0	34.0	40.0
20	38.143765275676365	40.0	38.0	40.0	34.0	40.0
21	38.32416440066446	40.0	38.0	40.0	35.0	40.0
22	38.36372840244199	40.0	38.0	40.0	35.0	40.0
23	38.303415932199805	40.0	38.0	40.0	35.0	40.0
24	38.25145548760488	40.0	38.0	40.0	35.0	40.0
25	38.20508488250294	40.0	38.0	40.0	34.0	40.0
26	38.12555878556389	40.0	38.0	40.0	34.0	40.0
27	38.01882611386793	40.0	38.0	40.0	34.0	40.0
28	37.94146109529906	40.0	38.0	40.0	34.0	40.0
29	37.73288595219705	40.0	38.0	40.0	34.0	40.0
30	37.74098338323829	40.0	37.0	40.0	33.0	40.0
31	37.694109540486494	40.0	37.0	40.0	33.0	40.0
32	37.645144715541775	40.0	37.0	40.0	33.0	40.0
33	37.289366938939615	40.0	37.0	40.0	33.0	40.0
34	37.29252721847788	40.0	36.0	40.0	33.0	40.0
35	37.21820344820289	40.0	36.0	40.0	33.0	40.0
36	37.14979619205823	39.0	36.0	40.0	32.0	40.0
37	36.77907338143959	39.0	35.0	40.0	31.0	40.0
38	36.78370568070001	39.0	35.0	40.0	31.0	40.0
39	36.38267090241554	39.0	35.0	40.0	31.0	40.0
40	36.32559475945108	39.0	35.0	40.0	30.0	40.0
41	36.266541375260545	39.0	35.0	40.0	30.0	40.0
42	36.25900008464444	38.0	35.0	40.0	30.0	40.0
43	36.15888621142065	38.0	35.0	40.0	30.0	40.0
44	36.34643184006433	39.0	35.0	40.0	31.0	40.0
45	36.43941375789574	39.0	35.0	40.0	31.0	40.0
46	36.30369962333224	38.0	35.0	40.0	31.0	40.0
47	35.96688947552189	38.0	35.0	40.0	31.0	40.0
48	35.94496193645319	38.0	35.0	40.0	31.0	40.0
49	35.850414757758195	37.0	35.0	40.0	30.0	40.0
50	35.347558669177786	37.0	35.0	40.0	29.0	40.0
51	35.389505940981664	37.0	35.0	40.0	29.0	40.0
52	35.04405676996815	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	12.0
16	19.0
17	75.0
18	135.0
19	296.0
20	517.0
21	873.0
22	1516.0
23	2468.0
24	3667.0
25	5189.0
26	7105.0
27	9850.0
28	13067.0
29	16560.0
30	21629.0
31	28161.0
32	37515.0
33	53664.0
34	75330.0
35	109141.0
36	171649.0
37	306402.0
38	422343.0
39	225021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.70090093426302	0.2563800746987187	0.03253520679694857	0.0101837842413213
8	99.51045094325647	0.41317067493360704	0.04377704654386169	0.032601335266048054
9	98.17359781194122	0.8978262249637615	0.2563800746987187	0.6721958883963053
10	54.99170748997493	29.55836763196597	5.5578333139356495	9.892091564123454
11	31.077470824119434	25.778199824362787	21.06489318929671	22.079436162221068
12	26.460844010876812	17.853959243701926	28.285989758022705	27.39920698739856
13	22.37992392580915	17.513397627839556	32.822402738247646	27.284275708103646
14	23.81940844116682	16.348676901590256	33.59597356977347	26.23594108746945
15	23.906499634970853	16.921349443991833	31.38113275422429	27.791018166813032
16	26.628479680044016	26.12186947827283	24.1717409245289	23.077909917154255
17	29.40580925375345	19.08421328282882	23.668106503867193	27.841870959550537
18	28.388158242781415	19.62858284045581	24.661752880556115	27.32150603620666
19	27.55037666775999	17.8830557701057	26.005351115719535	28.56121644641478
20	25.953043496661838	20.03758742183615	26.311525927650166	27.697843153851853
21	28.417519283061587	17.268061007480455	27.134560854062407	27.179858855395555
22	31.470604572915896	16.962481351771714	25.080147704548583	26.48676637076381
23	29.594010876810593	21.792769248674784	22.73033868356734	25.882881190947277
24	26.40423804132765	21.420399839175563	23.538825346777692	28.636536772719097
25	25.927187265243933	21.769690412959065	22.86147143779163	29.441650884005377
26	27.870835229016116	21.385285622083735	23.507083681609934	27.236795467290214
27	28.820638430692075	19.697158062911978	24.55607958693513	26.926123919460814
28	29.64955879085417	20.207802101298235	22.180877233819686	27.961761874027914
29	28.69433305471205	19.812419984552392	23.398831377694073	28.09441558304149
30	27.214708558610983	19.06212637414959	25.680329690095544	28.042835377143888
31	28.904886100324823	20.483293303566704	21.62043845820152	28.991382137906957
32	30.838085765979283	20.915046078317268	21.84520912467068	26.40165903103277
33	33.19695438722716	19.464650365558178	22.29468432913991	25.043710918074762
34	29.180641816469695	22.01694475892205	24.213997016283475	24.588416408324782
35	28.877641171055835	23.90239966988668	24.84334165670331	22.376617502354172
36	30.97669103721181	22.44704432194513	20.73101054881339	25.84525409202967
37	29.964793203051432	23.800429570535268	21.65006401235809	24.58471321405521
38	30.029202331954334	24.57664554082507	21.415109561647604	23.97904256557299
39	29.591497994984817	22.676576238189455	20.677975516595602	27.053950250230123
40	28.26588670341646	22.567794906520795	21.720490831949043	27.4458275581137
41	27.10903526499	21.51403775142044	21.690137864632376	29.68678911895718
42	26.137145154634812	21.59934347655878	22.706201792346025	29.557309576460383
43	28.20584205347412	20.653111212214192	24.07605302974194	27.06499370456974
44	26.848621353676215	21.607609535196218	24.62895315988277	26.9148159512448
45	28.84457693650609	20.30930930136595	22.54537735549607	28.300736406631895
46	27.822759831980786	20.76625702284342	23.918997915630655	27.49198522954514
47	27.44424047485531	21.338598922899497	24.004039126892597	27.213121475352597
48	28.526565128606645	22.613555807137644	21.70924899220213	27.150630072053577
49	26.528559563234687	22.301164919111656	23.063692296297862	28.10658322135579
50	26.48689862770201	24.04014527102092	23.308764402780568	26.164191698496502
51	27.328383396993004	24.385864907473046	23.13927713647858	25.14647455905537
52	28.039198311343412	24.519708928930413	21.839588204797224	25.60150455492895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	37.0
6	71.0
7	903.5
8	1736.0
9	2267.0
10	2798.0
11	2970.5
12	3143.0
13	2909.5
14	2861.0
15	3046.0
16	2736.5
17	2427.0
18	2363.0
19	2299.0
20	2981.5
21	3664.0
22	4892.5
23	6121.0
24	6197.0
25	6273.0
26	7538.5
27	8804.0
28	10518.0
29	12232.0
30	11062.5
31	9893.0
32	11951.5
33	14010.0
34	15894.5
35	17779.0
36	19497.0
37	21215.0
38	22901.0
39	28341.5
40	32096.0
41	38118.5
42	44141.0
43	52193.0
44	60245.0
45	75315.0
46	90385.0
47	79722.5
48	69060.0
49	76631.0
50	84202.0
51	92576.0
52	100950.0
53	113641.5
54	126333.0
55	133339.0
56	140345.0
57	139277.0
58	138209.0
59	132378.0
60	126547.0
61	115826.5
62	105106.0
63	92196.5
64	65654.5
65	52022.0
66	54410.5
67	56799.0
68	42102.5
69	27406.0
70	23423.5
71	19441.0
72	14860.5
73	10280.0
74	7069.5
75	3859.0
76	2872.0
77	1885.0
78	1276.5
79	668.0
80	379.0
81	90.0
82	75.5
83	61.0
84	36.5
85	12.0
86	6.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1512208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.29564180440806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74970346185056	7.593005300245266
2	9.990454365708363	2.057162792252361
3	3.857792395275024	1.1915534597256314
4	1.9725195924065904	0.8123342070238097
5	1.1200758186790016	0.5765949711449055
6	0.6920508378798528	0.42750645223508627
7	0.48633955151663916	0.3505024472412245
8	0.29297124723421597	0.24130616164113305
9	0.24084800073409413	0.22317162703794388
>10	2.7329883668132884	7.480683556010789
>50	2.333049512860104	17.605214266059768
>100	2.3800576400566036	43.513569502773855
>500	0.10558748570290614	7.286487574548768
>1k	0.041222511541545555	7.224910574506342
>5k	0.0036160097843461005	2.6149004407375656
>10k+	7.232019568692203E-4	0.8010966668155548
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	10759	0.7114761990414017	No Hit
TTAACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	9721	0.6428348481161322	No Hit
TTAACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	7654	0.5061473024874885	No Hit
TTAACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	6467	0.42765280966639513	No Hit
TTAACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	5863	0.38771121433030376	No Hit
TTAACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	5414	0.35801953170463324	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4721	0.31219250261868736	No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4168	0.27562345920667	No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3380	0.22351422555627268	No Hit
TTAACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3352	0.22166262842148698	No Hit
TTAACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2850	0.18846613693354355	No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2666	0.17629849861923755	No Hit
TTAACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2630	0.17391787373165596	No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2555	0.16895823854919428	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2521	0.16670987059981165	No Hit
TTAACTGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA	2470	0.16333731867573775	No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2397	0.15850994043147504	No Hit
TTAACTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2376	0.15712124258038576	No Hit
TTAACTGGGTCTTTTTGTTCGGGGCTTGTTTTATTCAACTCCGAATTGAATG	2090	0.13820850041793192	No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	2081	0.13761334419603652	No Hit
TTAACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2080	0.137547215726937	No Hit
TTAACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1848	0.1222054108958556	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1801	0.1190973728481796	No Hit
TTAACTGGGTCTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTA	1736	0.11479902235671284	No Hit
TTAACTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	1700	0.11241839746913122	No Hit
TTAACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1696	0.11215388359273329	No Hit
TTAACTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1676	0.11083131421074349	No Hit
TTAACTGGGCTGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	1667	0.1102361579888481	No Hit
TTAACTGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	1635	0.10812004697766445	No Hit
TTAACTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA	1571	0.10388782495529716	No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1549	0.1024329986351084	No Hit
TTAACTGGGATCTCACCTAGATCTTTCTCCCCCAGGTCATCTCAGCTCAGTC	1528	0.10104430078401913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.9677081459693375E-4	0.0	0.0	0.0	0.0
8	4.628992836964227E-4	0.0	0.0	0.0	0.0
9	6.612846909948896E-4	0.0	0.0	0.0	0.0
10	0.004628992836964227	0.0	0.0	0.0	0.0
11	0.010911197401415678	0.0	0.0	0.0	0.0
12	0.016333731867573774	0.0	0.0	0.0	0.0
13	0.019772412260747198	0.0	0.0	0.0	0.0
14	0.028236856305481787	0.0	0.0	0.0	0.0
15	0.03650291494291791	0.0	0.0	0.0	0.0
16	0.04443833123485658	0.0	0.0	0.0	0.0
17	0.04992699417011416	0.0	0.0	0.0	0.0
18	0.055085014759874304	0.0	0.0	0.0	0.0
19	0.06123496238612677	0.0	0.0	0.0	0.0
20	0.0670542676668818	0.0	0.0	0.0	0.0
21	0.07194777438024398	0.0	0.0	0.0	0.0
22	0.07677515262450668	0.0	0.0	0.0	0.0
23	0.08312348565805762	0.0	0.0	0.0	0.0
24	0.092778242146583	0.0	0.0	0.0	0.0
25	0.10038301609302425	0.0	0.0	0.0	0.0
26	0.1078555331012665	0.0	0.0	0.0	0.0
27	0.11413773766571794	0.0	0.0	0.0	0.0
28	0.12551183435083005	0.0	0.0	0.0	0.0
29	0.13133113963158508	0.0	0.0	0.0	0.0
30	0.1371504449123401	0.0	0.0	0.0	0.0
31	0.14382942029138848	0.0	0.0	0.0	0.0
32	0.14984711097944198	0.0	0.0	0.0	0.0
33	0.15645995788939088	0.0	0.0	0.0	0.0
34	0.1634695756139367	0.0	0.0	0.0	0.0
35	0.1761662416810386	0.0	0.0	0.0	0.0
36	0.18423391491117624	0.0	0.0	0.0	0.0
37	0.19058224794472717	0.0	0.0	0.0	0.0
38	0.1964676816945817	0.0	0.0	0.0	0.0
39	0.20341117095002803	0.0	0.0	0.0	0.0
40	0.2125368996857575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGT	40	5.6134013E-9	46.0	27
GTCGATA	20	6.31209E-4	46.0	29
CGACGTA	25	3.4176708E-5	46.0	38
CGCATTC	25	3.4176708E-5	46.0	37
ATTCGTT	25	3.4176708E-5	46.0	41
CGCGAAT	20	6.31209E-4	46.0	16
TTATCCG	20	6.31209E-4	46.0	14
ACCTATA	30	1.8617957E-6	46.0	44
GCCGATA	20	6.31209E-4	46.0	11
ATATCGT	20	6.31209E-4	46.0	37
ACGATAC	20	6.31209E-4	46.0	45
TCTAACG	30	1.8617957E-6	46.0	13
AACGATA	20	6.31209E-4	46.0	44
AATTCGG	20	6.31209E-4	46.0	33
AATTCGC	30	1.8617957E-6	46.0	16
CGTACAC	25	3.4176708E-5	46.0	10
TATAGGG	25	3.4176708E-5	46.0	34
GTATACC	25	3.4176708E-5	46.0	31
TTACGAT	20	6.31209E-4	46.0	14
ACGTATA	25	3.4176708E-5	46.0	16
>>END_MODULE