FastQCFastQC Report
Fri 17 Jun 2016
SRR1527127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1467231
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT109260.7446680175105352No Hit
TTAACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG91380.6228058158531274No Hit
TTAACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC73240.49917156875774843No Hit
TTAACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA60760.4141133877351283No Hit
TTAACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG60330.41118269720309897No Hit
TTAACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA53660.36572291615975944No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA44250.3015885024239537No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC42200.28761660570148806No Hit
TTAACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG33100.22559501537249416No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33100.22559501537249416No Hit
TTAACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA28140.19178984086350412No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG27590.1880412832062572No Hit
TTAACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA25980.17706823260958907No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24570.16745829388828343No Hit
TTAACTGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA24560.16739013829451532No Hit
TTAACTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG23830.1624147799494422No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG23290.15873437788596342No Hit
TTAACTGGGTCTTTTTGTTCGGGGCTTGTTTTATTCAACTCCGAATTGAATG21200.14448985878842527No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC21020.143263058100599No Hit
TTAACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC20220.137810610599149No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT18880.12867776103422024No Hit
TTAACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC18700.127450960346394No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17830.12152142368856711No Hit
TTAACTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT17780.12118064571972648No Hit
TTAACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA16910.1152511090618996No Hit
TTAACTGGGCTGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA16790.11443324193668208No Hit
TTAACTGGGTCTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTA16400.1117751737797252No Hit
TTAACTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16400.1117751737797252No Hit
TTAACTGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT15670.10679981543465207No Hit
TTAACTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA14800.1008702787768252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC206.3120277E-446.025
GTCGATA253.4176213E-546.029
CGAAATC600.046.025
AGCTTAC301.8617575E-646.037
TAGCGAA206.3120277E-446.020
TCGATAG253.4176213E-546.030
CCGTAGA206.3120277E-446.019
TCTACGT206.3120277E-446.021
TAACACA206.3120277E-446.022
TCTAACG253.4176213E-546.013
GTACTAG253.4176213E-546.040
AATTCGC206.3120277E-446.016
CGTACCG206.3120277E-446.041
TCGTACA206.3120277E-446.019
TCGTAAT206.3120277E-446.011
GTATATC206.3120277E-446.040
CGTAATC206.3120277E-446.012
CACGTTA206.3120277E-446.022
TAAGCGC405.6134013E-946.038
TGTAACG206.3120277E-446.037
Produced by FastQC (version 0.11.5)