##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527127_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1467231
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.364372072291275	33.0	31.0	34.0	31.0	34.0
2	32.831808351922774	34.0	31.0	34.0	31.0	34.0
3	32.68013353043931	34.0	31.0	34.0	31.0	34.0
4	36.48024203414459	37.0	37.0	37.0	35.0	37.0
5	36.41044252745478	37.0	37.0	37.0	35.0	37.0
6	36.38611506981518	37.0	37.0	37.0	35.0	37.0
7	36.51320821329429	37.0	37.0	37.0	35.0	37.0
8	36.64457062316704	37.0	37.0	37.0	35.0	37.0
9	38.66613096369965	39.0	39.0	39.0	39.0	39.0
10	38.1697285567167	39.0	39.0	39.0	37.0	39.0
11	37.8032163987811	39.0	38.0	39.0	35.0	39.0
12	37.57660995439709	39.0	37.0	39.0	35.0	39.0
13	37.45564536190961	39.0	37.0	39.0	35.0	39.0
14	38.32752920296804	40.0	38.0	40.0	35.0	40.0
15	38.40934249617136	40.0	38.0	40.0	35.0	40.0
16	38.494789164078455	40.0	38.0	40.0	35.0	40.0
17	38.45972788197632	40.0	38.0	40.0	35.0	40.0
18	38.4374655388279	40.0	38.0	40.0	35.0	40.0
19	38.42942113409545	40.0	38.0	40.0	35.0	40.0
20	38.3696957057205	40.0	38.0	40.0	35.0	40.0
21	34.099063473986035	40.0	35.0	40.0	10.0	40.0
22	34.935098154278364	40.0	35.0	40.0	18.0	40.0
23	36.959773887002115	40.0	35.0	40.0	31.0	40.0
24	37.670099663924766	40.0	37.0	40.0	33.0	40.0
25	37.93106743246292	40.0	38.0	40.0	34.0	40.0
26	38.016972105960136	40.0	38.0	40.0	34.0	40.0
27	37.99462184209576	40.0	38.0	40.0	34.0	40.0
28	37.92085295362489	40.0	38.0	40.0	34.0	40.0
29	37.865735524944604	40.0	38.0	40.0	34.0	40.0
30	37.805029337575334	40.0	38.0	40.0	34.0	40.0
31	37.66921227809391	40.0	37.0	40.0	33.0	40.0
32	37.64248301732992	40.0	37.0	40.0	33.0	40.0
33	37.31471049889213	40.0	37.0	40.0	33.0	40.0
34	37.29708955167932	40.0	36.0	40.0	33.0	40.0
35	37.225016374381404	40.0	36.0	40.0	33.0	40.0
36	37.09366486940366	39.0	36.0	40.0	32.0	40.0
37	36.93711419674202	39.0	35.0	40.0	32.0	40.0
38	36.87363271359452	39.0	35.0	40.0	32.0	40.0
39	36.69991228375082	39.0	35.0	40.0	31.0	40.0
40	31.75062754262962	38.0	31.0	40.0	9.0	40.0
41	32.67072328760774	38.0	31.0	40.0	15.0	40.0
42	34.541614101664976	37.0	32.0	40.0	25.0	40.0
43	35.40493555547831	37.0	34.0	40.0	28.0	40.0
44	36.04723250803725	38.0	35.0	40.0	30.0	40.0
45	36.32009342768794	39.0	35.0	40.0	31.0	40.0
46	36.27697547284647	38.0	35.0	40.0	31.0	40.0
47	36.18535458969992	38.0	35.0	40.0	31.0	40.0
48	36.06831030696598	38.0	35.0	40.0	31.0	40.0
49	35.912946904747784	37.0	35.0	40.0	31.0	40.0
50	35.80923385615489	37.0	35.0	40.0	31.0	40.0
51	35.59384037005761	37.0	35.0	40.0	30.0	40.0
52	35.19626902648594	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	6.0
16	24.0
17	62.0
18	127.0
19	305.0
20	545.0
21	996.0
22	1629.0
23	2582.0
24	3798.0
25	5418.0
26	7479.0
27	10016.0
28	13651.0
29	17468.0
30	23492.0
31	30580.0
32	41816.0
33	55547.0
34	88993.0
35	131985.0
36	192557.0
37	204855.0
38	403813.0
39	229483.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.70277345557722	0.25449298713017926	0.03128341753956943	0.011450139753045022
8	99.52625046771776	0.4194295240490421	0.024263391381452545	0.030056616851743184
9	98.11161296346656	0.9132168008991085	0.28986574029583617	0.6853044953384982
10	55.006948462784656	29.542996297106587	5.548819511038139	9.90123572907061
11	31.07704240163955	25.771129426791013	21.069892879853274	22.081935291716164
12	26.480015757573277	17.8808926474427	28.30890296074715	27.330188634236873
13	22.523583539333618	17.543045369134102	32.73199652951717	27.20137456201512
14	23.805385791330743	16.377312093324093	33.655232202700184	26.162069912644974
15	23.909595694202206	16.709025368193554	31.494359102281784	27.88701983532245
16	27.681598875705326	17.941755592677637	27.56328076492386	26.813364766693184
17	29.445533798018168	19.122823877085477	23.60773456940318	27.82390775549317
18	28.367107837825127	19.69015103961135	24.601238659761144	27.34150246280238
19	27.5058937549711	17.893774054664878	26.013695185011766	28.586637005352262
20	25.928159914832772	20.082863570903285	26.39338999789399	27.595586516369952
21	33.11837059058867	17.089674359388535	25.464838188397053	24.32711686162574
22	31.526119608977726	17.015861851337654	25.062788340758885	26.395230198925734
23	29.698050272929077	21.806654848486705	22.721302916854945	25.77399196172927
24	26.495418921764873	21.491298916121593	23.548234736043607	28.465047426069923
25	25.953922729277124	21.732978651623363	22.827557487539455	29.485541131560062
26	27.86009837578404	21.474532640054633	23.4684926913349	27.19687629282642
27	28.781153069966486	19.75523963165991	24.587403074226213	26.876204224147394
28	29.494605825531224	20.306413918462738	22.239511024508072	27.95946923149797
29	28.61976062392357	19.766689771412953	23.477421074118528	28.13612853054495
30	27.17642961469598	19.09140414835837	25.692137093613752	28.040029143331896
31	28.96687706298463	20.479460971040005	21.577992831394646	28.975669134580716
32	30.807146250317775	20.945917854789055	21.880603667725122	26.366332227168048
33	33.08974524120605	19.53755066516452	22.270998908828943	25.101705184800483
34	29.16936733206973	22.104903726816023	24.15890885620601	24.56682008490824
35	28.9079224743752	23.97938702222077	24.772036577744064	22.340653925659968
36	30.99954949152519	22.49182303263767	20.62763123189191	25.880996243945226
37	29.798443462549525	23.818062731771615	21.687859648548866	24.695634157129994
38	30.051641493398108	24.618618336172013	21.412306582944336	23.917433587485544
39	29.380990450719757	22.66016734924494	20.705805697943948	27.253036502091355
40	28.59754190035516	27.115634825054812	20.061530870053865	24.225292404536162
41	27.07058397757408	21.527898469975078	21.6989008547393	29.702616697711537
42	26.057791854179747	21.700604744583504	22.679591693468854	29.562011707767898
43	28.173750418304955	20.67527199193583	24.10990498428673	27.041072605472483
44	26.8486012086713	21.582422944989577	24.717443947135795	26.85153189920333
45	28.864234738769834	20.340968804503177	22.57211032209652	28.22268613463047
46	27.94570180155681	20.67595354787351	23.91954641089235	27.458798239677325
47	27.17690670385236	21.382181810498825	24.027709338202367	27.413202147446448
48	28.48215448010572	22.619955548921745	21.729979805497564	27.167910165474968
49	26.518728134833573	22.295398611397932	23.060785929413978	28.125087324354514
50	26.41158754143008	23.942446690398445	23.463517332989827	26.182448435181644
51	27.312331868669627	24.37523471082604	23.130168323869928	25.18226509663441
52	27.956402229778405	24.566615618126935	21.86881275000324	25.608169402091423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	3.0
4	5.0
5	27.0
6	49.0
7	816.0
8	1583.0
9	2074.5
10	2566.0
11	2756.0
12	2946.0
13	2785.5
14	2849.0
15	3073.0
16	2722.5
17	2372.0
18	2326.5
19	2281.0
20	2918.0
21	3555.0
22	4662.5
23	5770.0
24	5900.0
25	6030.0
26	7354.5
27	8679.0
28	10035.0
29	11391.0
30	10453.0
31	9515.0
32	11534.0
33	13553.0
34	15343.0
35	17133.0
36	19031.0
37	20929.0
38	22136.0
39	26972.0
40	30601.0
41	36847.0
42	43093.0
43	50340.0
44	57587.0
45	72649.0
46	87711.0
47	77741.5
48	67772.0
49	74782.0
50	81792.0
51	89320.0
52	96848.0
53	110066.0
54	123284.0
55	129723.0
56	136162.0
57	134576.5
58	132991.0
59	127963.5
60	122936.0
61	112121.5
62	101307.0
63	90112.0
64	64728.0
65	50539.0
66	53256.0
67	55973.0
68	41143.5
69	26314.0
70	22523.5
71	18733.0
72	14563.0
73	10393.0
74	7234.0
75	4075.0
76	3025.5
77	1976.0
78	1317.0
79	658.0
80	381.0
81	104.0
82	79.5
83	55.0
84	31.0
85	7.0
86	5.0
87	3.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1467231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.00599139906537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9914564730983	7.203458942760105
2	9.385522751047109	1.8782291984541948
3	4.031880785942181	1.2102889339858316
4	2.2570662446983674	0.9033674172629056
5	1.4291177655561402	0.7149870035203131
6	0.9002619276009991	0.5404807862688965
7	0.6045076624272478	0.4234088929641308
8	0.4553490817988867	0.36449751968415794
9	0.34791349291389567	0.31330974759437075
>10	3.572033140624384	9.077167526142857
>50	2.5018570666104263	18.335720747860275
>100	2.3770667352952146	42.0067379515226
>500	0.10437009528181343	7.108078146727392
>1k	0.037058946875426504	6.525223003574186
>5k	0.003781525191370052	2.5682102042573836
>10k+	7.563050382740103E-4	0.8268339774204017
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTAACTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT	10926	0.7446680175105352	No Hit
TTAACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	9138	0.6228058158531274	No Hit
TTAACTGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC	7324	0.49917156875774843	No Hit
TTAACTGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA	6076	0.4141133877351283	No Hit
TTAACTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	6033	0.41118269720309897	No Hit
TTAACTGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA	5366	0.36572291615975944	No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4425	0.3015885024239537	No Hit
TTAACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4220	0.28761660570148806	No Hit
TTAACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3310	0.22559501537249416	No Hit
TTAACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3310	0.22559501537249416	No Hit
TTAACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2814	0.19178984086350412	No Hit
TTAACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2759	0.1880412832062572	No Hit
TTAACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2598	0.17706823260958907	No Hit
TTAACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2457	0.16745829388828343	No Hit
TTAACTGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA	2456	0.16739013829451532	No Hit
TTAACTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2383	0.1624147799494422	No Hit
TTAACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2329	0.15873437788596342	No Hit
TTAACTGGGTCTTTTTGTTCGGGGCTTGTTTTATTCAACTCCGAATTGAATG	2120	0.14448985878842527	No Hit
TTAACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2102	0.143263058100599	No Hit
TTAACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	2022	0.137810610599149	No Hit
TTAACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	1888	0.12867776103422024	No Hit
TTAACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1870	0.127450960346394	No Hit
TTAACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1783	0.12152142368856711	No Hit
TTAACTGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT	1778	0.12118064571972648	No Hit
TTAACTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1691	0.1152511090618996	No Hit
TTAACTGGGCTGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAACCTA	1679	0.11443324193668208	No Hit
TTAACTGGGTCTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTA	1640	0.1117751737797252	No Hit
TTAACTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1640	0.1117751737797252	No Hit
TTAACTGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	1567	0.10679981543465207	No Hit
TTAACTGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCAGA	1480	0.1008702787768252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.726223750725005E-4	0.0	0.0	0.0	0.0
8	4.770891563768759E-4	0.0	0.0	0.0	0.0
9	8.860227189856267E-4	0.0	0.0	0.0	0.0
10	0.005793225470290636	0.0	0.0	0.0	0.0
11	0.012268006878262523	0.0	0.0	0.0	0.0
12	0.018265699129857534	0.0	0.0	0.0	0.0
13	0.022014256787104416	0.0	0.0	0.0	0.0
14	0.02910243853898943	0.0	0.0	0.0	0.0
15	0.03707664300986007	0.0	0.0	0.0	0.0
16	0.04532346985580321	0.0	0.0	0.0	0.0
17	0.05057145057594885	0.0	0.0	0.0	0.0
18	0.054660786202036356	0.0	0.0	0.0	0.0
19	0.06024954489102261	0.0	0.0	0.0	0.0
20	0.06611092595508138	0.0	0.0	0.0	0.0
21	0.07033657276870513	0.0	0.0	0.0	0.0
22	0.07606164264522765	0.0	0.0	0.0	0.0
23	0.08199117930305452	0.0	0.0	0.0	0.0
24	0.09173742921189643	0.0	0.0	0.0	0.0
25	0.09923454452639019	0.0	0.0	0.0	0.0
26	0.10618641509073895	0.0	0.0	0.0	0.0
27	0.11381984159276896	0.0	0.0	0.0	0.0
28	0.12417949184552399	0.0	0.0	0.0	0.0
29	0.12895038340929274	0.0	0.0	0.0	0.0
30	0.13467545328581526	0.0	0.0	0.0	0.0
31	0.14080945672494652	0.0	0.0	0.0	0.0
32	0.147079771351614	0.0	0.0	0.0	0.0
33	0.1532137747907453	0.0	0.0	0.0	0.0
34	0.16050642332393467	0.0	0.0	0.0	0.0
35	0.17284258579596531	0.0	0.0	0.0	0.0
36	0.17972630076654597	0.0	0.0	0.0	0.0
37	0.1866100157371266	0.0	0.0	0.0	0.0
38	0.193357419520171	0.0	0.0	0.0	0.0
39	0.2007182236471285	0.0	0.0	0.0	0.0
40	0.2093739840556804	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	6.3120277E-4	46.0	25
GTCGATA	25	3.4176213E-5	46.0	29
CGAAATC	60	0.0	46.0	25
AGCTTAC	30	1.8617575E-6	46.0	37
TAGCGAA	20	6.3120277E-4	46.0	20
TCGATAG	25	3.4176213E-5	46.0	30
CCGTAGA	20	6.3120277E-4	46.0	19
TCTACGT	20	6.3120277E-4	46.0	21
TAACACA	20	6.3120277E-4	46.0	22
TCTAACG	25	3.4176213E-5	46.0	13
GTACTAG	25	3.4176213E-5	46.0	40
AATTCGC	20	6.3120277E-4	46.0	16
CGTACCG	20	6.3120277E-4	46.0	41
TCGTACA	20	6.3120277E-4	46.0	19
TCGTAAT	20	6.3120277E-4	46.0	11
GTATATC	20	6.3120277E-4	46.0	40
CGTAATC	20	6.3120277E-4	46.0	12
CACGTTA	20	6.3120277E-4	46.0	22
TAAGCGC	40	5.6134013E-9	46.0	38
TGTAACG	20	6.3120277E-4	46.0	37
>>END_MODULE