FastQCFastQC Report
Fri 17 Jun 2016
SRR1527126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915192
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT98931.0809753581762078No Hit
TAGAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC42400.46329076303114536No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA33360.3645136758188446No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA29770.325286934326349No Hit
TAGAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG25610.27983199153838756No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25090.2741501236898924No Hit
TAGAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC24790.2708721230080683No Hit
TAGAGAGGGATTTTTCTTTCTGTCTTCTCCCAGGTCCCTTACGACAGACT23450.25623038662925374No Hit
TAGAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG21970.2400589165989213No Hit
TAGAGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT20680.22596351366707754No Hit
TAGAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT19900.21744071189433475No Hit
TAGAGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC19890.2173314452049406No Hit
TAGAGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT19010.20771597653825644No Hit
TAGAGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA17990.19657077422005437No Hit
TAGAGAGGGTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAAT17310.18914063934125297No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA15970.1744989029624385No Hit
TAGAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG15530.16969116862909642No Hit
TAGAGAGGGAGAGTCCATACCGGGGAGAGAACGTACAACTACAAGAAGGG15450.1688170351139433No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC15330.16750583484121365No Hit
TAGAGAGGGATGGGTAGTAAATACCAATGTAGTCAGTGTTCGTGTTGTGA14470.15810889955331778No Hit
TAGAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC14160.1547216321820995No Hit
TAGAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG14140.1545030988033112No Hit
TAGAGAGAGTTAAATGGGAACTAGTATATGTGAAGAACTCTTTCTGTATT14010.15308263184118742No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA13870.1515528981896695No Hit
TAGAGAGGGGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTC13650.14914903102299845No Hit
TAGAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT13470.14718223061390398No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA13290.14521543020480948No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12870.14062622925025567No Hit
TAGAGAGGGGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAAC12750.13931502897752604No Hit
TAGAGAGGGGAATTGTTATCTCGTTCACGCATGAGCTGTTTAAAAATTAC12740.1392057622881319No Hit
TAGAGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG12480.1363648283638843No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT12150.13275902761387776No Hit
TAGAGAGGGATTTTTGAGACTGTATTCCCCAGCATATATCTTCTAACCTT12040.13155709403054222No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC12010.13122929396235983No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTT11460.12521962604568224No Hit
TAGAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT11390.12445475921992324No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA11090.12117675853809912No Hit
TAGAGAGGGCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCC11080.12106749184870498No Hit
TAGAGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG10940.11953775819718702No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10930.11942849150779289No Hit
TAGAGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC10900.1191006914396105No Hit
TAGAGAGGGCAGGAACCTTGCGGGCGACTCTCATTTATGAAGGAACCCAA10860.11866362468203394No Hit
TAGAGAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA10760.11757095778809254No Hit
TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAA10660.11647829089415117No Hit
TAGAGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT10560.11538562400020978No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10280.11232615669717393No Hit
TAGAGAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA10200.11145202318202081No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCAC10090.1102500895986853No Hit
TAGAGAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC10080.11014082290929117No Hit
TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC10000.10926668939413806No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC9940.10861108925777324No Hit
TAGAGAGGGAGTGGGGGTTGATTTGTGTGTTGGTTGATTGGTTAGTTACA9630.10522382188655494No Hit
TAGAGAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC9470.10347555485624874No Hit
TAGAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT9360.10227362127291323No Hit
TAGAGAGGGGAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC9210.10063462093200116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA1650.044.00000429
TCACGGG351.44566E-744.023
TCACGAA207.8556396E-444.041
ATTTACG351.44566E-744.043
AACGTTC207.8556396E-444.040
AGGTATC302.5271493E-644.026
TGATATA454.802132E-1044.029
AAGTACG302.5271493E-644.029
TTAGGAT254.442127E-544.044
TGATACT207.8556396E-444.043
AATCCGG254.442127E-544.013
TCGTTGA502.7284841E-1144.036
CCTTACG2900.044.037
ATCTCGT850.044.018
AATCCCG600.044.041
GTACCGC351.44566E-744.027
ACCGGTT254.442127E-544.039
AAATCCG207.8556396E-444.031
CGAACGT207.8556396E-444.038
CGAACAC207.8556396E-444.041
Produced by FastQC (version 0.11.5)