##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527126_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 915192 Sequences flagged as poor quality 0 Sequence length 50 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35796641579035 33.0 31.0 34.0 31.0 34.0 2 32.59201238647191 34.0 31.0 34.0 31.0 34.0 3 32.932110420545634 34.0 31.0 34.0 31.0 34.0 4 36.04903342686562 37.0 35.0 37.0 35.0 37.0 5 36.25303324329758 37.0 37.0 37.0 35.0 37.0 6 35.81814963417512 37.0 35.0 37.0 35.0 37.0 7 36.45360973435083 37.0 37.0 37.0 35.0 37.0 8 36.65987355658703 37.0 37.0 37.0 35.0 37.0 9 38.7463198979012 39.0 39.0 39.0 39.0 39.0 10 38.2132273883513 39.0 39.0 39.0 37.0 39.0 11 37.86880894937893 39.0 38.0 39.0 35.0 39.0 12 37.4726418063095 39.0 37.0 39.0 35.0 39.0 13 37.351171120376925 39.0 37.0 39.0 35.0 39.0 14 38.21277830225789 40.0 38.0 40.0 35.0 40.0 15 38.159779587234155 40.0 38.0 40.0 34.0 40.0 16 38.03825536062378 40.0 38.0 40.0 34.0 40.0 17 38.113854797681796 40.0 38.0 40.0 34.0 40.0 18 38.02118899640731 40.0 38.0 40.0 34.0 40.0 19 38.00228039580765 40.0 38.0 40.0 34.0 40.0 20 37.769899649472464 40.0 38.0 40.0 33.0 40.0 21 37.96136548396402 40.0 38.0 40.0 34.0 40.0 22 37.787439138454005 40.0 38.0 40.0 34.0 40.0 23 37.776930960934976 40.0 37.0 40.0 33.0 40.0 24 37.767579917656626 40.0 37.0 40.0 34.0 40.0 25 37.6602953260081 40.0 37.0 40.0 33.0 40.0 26 37.45322948627173 40.0 37.0 40.0 33.0 40.0 27 37.34906336593851 40.0 36.0 40.0 33.0 40.0 28 37.15993256059931 39.0 36.0 40.0 32.0 40.0 29 37.388352389443966 40.0 37.0 40.0 33.0 40.0 30 37.384386008618954 40.0 36.0 40.0 33.0 40.0 31 37.37562828346402 40.0 36.0 40.0 33.0 40.0 32 36.61326475755907 40.0 35.0 40.0 31.0 40.0 33 36.81688978924641 40.0 35.0 40.0 31.0 40.0 34 36.66778118689849 39.0 35.0 40.0 31.0 40.0 35 36.666650276663255 39.0 35.0 40.0 31.0 40.0 36 36.41800627627864 39.0 35.0 40.0 31.0 40.0 37 36.236434540511716 39.0 35.0 40.0 30.0 40.0 38 36.00523715242266 39.0 35.0 40.0 30.0 40.0 39 35.90192768293429 39.0 35.0 40.0 30.0 40.0 40 35.71439435659403 38.0 35.0 40.0 29.0 40.0 41 35.566021119065724 38.0 35.0 40.0 29.0 40.0 42 35.44722091102195 38.0 35.0 40.0 29.0 40.0 43 35.23024567522443 37.0 35.0 40.0 28.0 40.0 44 35.085737200500006 37.0 34.0 40.0 28.0 40.0 45 34.89523400554201 37.0 34.0 40.0 27.0 40.0 46 34.56494156417451 36.0 34.0 40.0 26.0 40.0 47 34.427078689499034 36.0 34.0 40.0 26.0 40.0 48 34.28020677628301 35.0 34.0 39.0 26.0 40.0 49 33.877721833232805 35.0 33.0 39.0 24.0 40.0 50 33.47567614227397 35.0 33.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 0.0 12 1.0 13 2.0 14 2.0 15 2.0 16 24.0 17 79.0 18 186.0 19 402.0 20 744.0 21 1147.0 22 1717.0 23 2350.0 24 3110.0 25 4436.0 26 5925.0 27 8272.0 28 11336.0 29 14221.0 30 16808.0 31 19748.0 32 24061.0 33 32665.0 34 54456.0 35 67816.0 36 97838.0 37 165878.0 38 267843.0 39 114122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.56850584358254 0.3397101373263752 0.05528894483343386 0.03649507425764211 8 99.23709997465012 0.6215089292738573 0.05015341043190937 0.09123768564410528 9 98.49594402048969 1.0111539436533536 0.19733564104581336 0.2955663948111435 10 65.25985804071713 21.88054528448675 4.262165753197143 8.597430921598965 11 32.09687147614927 29.472394863591468 16.44704062098445 21.98369303927482 12 27.95970681561902 19.583650206732575 27.828149721588474 24.62849325605993 13 20.8786790094319 19.131286112640844 31.192471088033987 28.797563789893267 14 23.050245194450998 20.313660958574815 31.693131058837924 24.94296278813626 15 24.243655976013777 18.926957403473807 30.10406559497898 26.72532102553344 16 29.645254766212993 18.758249635049257 26.897088261261025 24.699407337476725 17 31.3451166531176 20.077644909483475 21.300229897114487 27.27700854028444 18 28.658248760915743 21.448614061311723 23.124765076617805 26.768372101154732 19 30.08199372372136 20.474938592120562 21.117208192379305 28.325859491778775 20 28.085691308490457 22.144642872752385 21.719267650941003 28.050398167816155 21 29.95240343009991 20.46543239014327 24.213716903119785 25.368447276637035 22 32.735098208900425 18.868062657890366 22.307996573396622 26.088842559812587 23 30.516984414199428 22.729875261147388 21.56684061923618 25.186299705417003 24 29.741627986258624 21.12212519340204 22.303625905820855 26.832620914518486 25 29.56275841572042 20.885453544174336 22.496153812533326 27.055634227571918 26 29.213760609795543 21.29400169581902 21.541600013986137 27.950637680399304 27 28.251448876301367 20.348517032491543 23.557679699997376 27.84235439120971 28 30.86314128620005 21.5870549567741 20.9364810881214 26.61332266890445 29 31.59424470493623 21.79728406716842 20.883377477075847 25.7250937508195 30 28.710587505135532 22.741676063601954 21.04869797812918 27.499038453133334 31 31.437447005655645 23.12869867743599 19.216514130368274 26.217340186540095 32 32.12648274897508 23.130337677776904 20.446092186120506 24.29708738712751 33 33.326012465143926 21.42435685626623 21.0017133016897 24.247917376900148 34 29.624275561849316 23.32111731745907 23.559209433648896 23.49539768704272 35 30.467923670661456 25.034746807227336 22.76342013479139 21.73390938731982 36 31.282288306715966 24.657448928749375 21.087706186242887 22.97255657829177 37 30.402909990471944 25.950729464418394 19.339111355868496 24.307249189241166 38 33.81509016687209 24.408867210377714 18.85265605468579 22.923386568064405 39 30.364775915873395 25.23317511516709 19.310155683179048 25.09189328578047 40 28.373936835112197 24.70825793931765 22.45353980366961 24.464265421900542 41 27.73844176959589 24.475192090839958 23.285605643405972 24.500760496158183 42 27.74106417014135 23.971144852664796 21.822743205797256 26.465047771396605 43 28.202169599384607 22.131421603335692 24.772288219302617 24.89412057797708 44 27.027771221776415 22.887110027185553 23.249219835837724 26.835898915200307 45 27.508326121731834 23.693279661535502 23.019541254731248 25.778852962001412 46 27.97882848626299 24.696894203620662 22.57176636159407 24.75251094852228 47 29.108973854666566 23.066525931170727 23.10116347170867 24.72333674245404 48 29.922245823827133 23.686177326724884 21.406874185963165 24.98470266348482 49 27.74401437075499 24.705963338840377 23.49911275448212 24.050909535922514 50 24.801571692060246 25.831847306357574 24.316646124529058 25.049934877053122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 16.5 6 33.0 7 78.0 8 123.0 9 1570.0 10 3017.0 11 2675.0 12 2333.0 13 1945.0 14 1557.0 15 1589.5 16 1622.0 17 1642.5 18 1663.0 19 2043.0 20 2423.0 21 2397.0 22 2371.0 23 2786.5 24 3202.0 25 4288.5 26 5375.0 27 6753.0 28 8131.0 29 7641.0 30 7151.0 31 9041.0 32 10931.0 33 11555.0 34 12179.0 35 11905.0 36 11631.0 37 14730.5 38 17830.0 39 19588.0 40 21346.0 41 22670.5 42 23995.0 43 23977.5 44 23960.0 45 26104.0 46 28248.0 47 30039.0 48 31830.0 49 33936.0 50 36042.0 51 42177.0 52 48312.0 53 51696.5 54 55081.0 55 71531.0 56 87981.0 57 87110.5 58 86240.0 59 84611.0 60 82982.0 61 78401.5 62 73821.0 63 70423.5 64 67026.0 65 56927.0 66 46828.0 67 39762.5 68 32697.0 69 29872.0 70 27047.0 71 23406.5 72 19766.0 73 19042.0 74 18318.0 75 13492.0 76 8666.0 77 5185.0 78 1704.0 79 1419.5 80 1135.0 81 777.5 82 420.0 83 262.5 84 105.0 85 81.5 86 58.0 87 35.0 88 12.0 89 6.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 915192.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.090062941104204 #Duplication Level Percentage of deduplicated Percentage of total 1 71.8431674213271 8.685884160121315 2 10.849784336442038 2.623491510499815 3 4.5674762182837965 1.6566322488314313 4 2.331932139572944 1.1277282536728275 5 1.3711600370727894 0.8288705575268399 6 0.8088030794311649 0.5867088082369012 7 0.5814969199064121 0.4921234053208727 8 0.3642342258534398 0.35228917726979603 9 0.29120479680253186 0.3168615889884663 >10 3.065409727351921 9.259178990351847 >50 1.8996776778712012 16.64036537412599 >100 1.896939074779604 41.19608992335473 >500 0.08398382814231163 6.722299697379124 >1k 0.043817649465553894 8.41962270458649 >5k 9.128676971990394E-4 1.0918535997335643 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 9893 1.0809753581762078 No Hit TAGAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 4240 0.46329076303114536 No Hit TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 3336 0.3645136758188446 No Hit TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGA 2977 0.325286934326349 No Hit TAGAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 2561 0.27983199153838756 No Hit TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2509 0.2741501236898924 No Hit TAGAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 2479 0.2708721230080683 No Hit TAGAGAGGGATTTTTCTTTCTGTCTTCTCCCAGGTCCCTTACGACAGACT 2345 0.25623038662925374 No Hit TAGAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG 2197 0.2400589165989213 No Hit TAGAGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT 2068 0.22596351366707754 No Hit TAGAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT 1990 0.21744071189433475 No Hit TAGAGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 1989 0.2173314452049406 No Hit TAGAGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT 1901 0.20771597653825644 No Hit TAGAGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA 1799 0.19657077422005437 No Hit TAGAGAGGGTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAAT 1731 0.18914063934125297 No Hit TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA 1597 0.1744989029624385 No Hit TAGAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG 1553 0.16969116862909642 No Hit TAGAGAGGGAGAGTCCATACCGGGGAGAGAACGTACAACTACAAGAAGGG 1545 0.1688170351139433 No Hit TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC 1533 0.16750583484121365 No Hit TAGAGAGGGATGGGTAGTAAATACCAATGTAGTCAGTGTTCGTGTTGTGA 1447 0.15810889955331778 No Hit TAGAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC 1416 0.1547216321820995 No Hit TAGAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 1414 0.1545030988033112 No Hit TAGAGAGAGTTAAATGGGAACTAGTATATGTGAAGAACTCTTTCTGTATT 1401 0.15308263184118742 No Hit TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA 1387 0.1515528981896695 No Hit TAGAGAGGGGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTC 1365 0.14914903102299845 No Hit TAGAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT 1347 0.14718223061390398 No Hit TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 1329 0.14521543020480948 No Hit TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT 1287 0.14062622925025567 No Hit TAGAGAGGGGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAAC 1275 0.13931502897752604 No Hit TAGAGAGGGGAATTGTTATCTCGTTCACGCATGAGCTGTTTAAAAATTAC 1274 0.1392057622881319 No Hit TAGAGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG 1248 0.1363648283638843 No Hit TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT 1215 0.13275902761387776 No Hit TAGAGAGGGATTTTTGAGACTGTATTCCCCAGCATATATCTTCTAACCTT 1204 0.13155709403054222 No Hit TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC 1201 0.13122929396235983 No Hit TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTT 1146 0.12521962604568224 No Hit TAGAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT 1139 0.12445475921992324 No Hit TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA 1109 0.12117675853809912 No Hit TAGAGAGGGCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCC 1108 0.12106749184870498 No Hit TAGAGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG 1094 0.11953775819718702 No Hit TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1093 0.11942849150779289 No Hit TAGAGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC 1090 0.1191006914396105 No Hit TAGAGAGGGCAGGAACCTTGCGGGCGACTCTCATTTATGAAGGAACCCAA 1086 0.11866362468203394 No Hit TAGAGAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA 1076 0.11757095778809254 No Hit TAGAGAGGGACGCAGAGTGCAGTGCTTAGAGAGGGAAAAAAAAAAAAAAA 1066 0.11647829089415117 No Hit TAGAGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT 1056 0.11538562400020978 No Hit TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 1028 0.11232615669717393 No Hit TAGAGAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCA 1020 0.11145202318202081 No Hit TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCAC 1009 0.1102500895986853 No Hit TAGAGAGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC 1008 0.11014082290929117 No Hit TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 1000 0.10926668939413806 No Hit TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC 994 0.10861108925777324 No Hit TAGAGAGGGAGTGGGGGTTGATTTGTGTGTTGGTTGATTGGTTAGTTACA 963 0.10522382188655494 No Hit TAGAGAGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC 947 0.10347555485624874 No Hit TAGAGAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT 936 0.10227362127291323 No Hit TAGAGAGGGGAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC 921 0.10063462093200116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.01791973706063864 0.0 0.0 0.0 0.0 5 0.01791973706063864 0.0 0.0 0.0 0.0 6 0.06457661343193559 0.0 0.0 0.0 0.0 7 0.06512294687890628 0.0 0.0 0.0 0.0 8 0.06785461411375973 0.0 0.0 0.0 0.0 9 0.07135114817437216 0.0 0.0 0.0 0.0 10 0.10216435458351909 0.0 0.0 0.0 0.0 11 0.15799963286392363 0.0 0.0 0.0 0.0 12 0.18553483859124642 0.0 0.0 0.0 0.0 13 0.2037823757200675 0.0 0.0 0.0 0.0 14 0.23361218192466718 0.0 0.0 0.0 0.0 15 0.25480991966712996 0.0 0.0 0.0 0.0 16 0.2744779237580748 0.0 0.0 0.0 0.0 17 0.29064939378840726 0.0 0.0 0.0 0.0 18 0.3044169966520686 0.0 0.0 0.0 0.0 19 0.32135333350816003 0.0 0.0 0.0 0.0 20 0.33643213664455107 0.0 0.0 0.0 0.0 21 0.3505275395763949 0.0 0.0 0.0 0.0 22 0.36910287677339837 0.0 0.0 0.0 0.0 23 0.38483728004615425 0.0 0.0 0.0 0.0 24 0.40406821737952253 0.0 0.0 0.0 0.0 25 0.4235176880916791 0.0 0.0 0.0 0.0 26 0.442420825356865 0.0 0.0 0.0 0.0 27 0.46121469593265674 0.0 0.0 0.0 0.0 28 0.49967657059939335 0.0 0.0 0.0 0.0 29 0.5189075079327616 0.0 0.0 0.0 0.0 30 0.5416349793267423 0.0 0.0 0.0 0.0 31 0.5586805828722279 0.0 0.0 0.0 0.0 32 0.5808617208192379 0.0 0.0 0.0 0.0 33 0.599109257948059 0.0 0.0 0.0 0.0 34 0.61418806108445 0.0 0.0 0.0 0.0 35 0.6617190709709001 0.0 0.0 0.0 0.0 36 0.683790942228516 0.0 0.0 0.0 0.0 37 0.7002902123270308 0.0 0.0 0.0 0.0 38 0.7173358158725164 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACGTA 165 0.0 44.000004 29 TCACGGG 35 1.44566E-7 44.0 23 TCACGAA 20 7.8556396E-4 44.0 41 ATTTACG 35 1.44566E-7 44.0 43 AACGTTC 20 7.8556396E-4 44.0 40 AGGTATC 30 2.5271493E-6 44.0 26 TGATATA 45 4.802132E-10 44.0 29 AAGTACG 30 2.5271493E-6 44.0 29 TTAGGAT 25 4.442127E-5 44.0 44 TGATACT 20 7.8556396E-4 44.0 43 AATCCGG 25 4.442127E-5 44.0 13 TCGTTGA 50 2.7284841E-11 44.0 36 CCTTACG 290 0.0 44.0 37 ATCTCGT 85 0.0 44.0 18 AATCCCG 60 0.0 44.0 41 GTACCGC 35 1.44566E-7 44.0 27 ACCGGTT 25 4.442127E-5 44.0 39 AAATCCG 20 7.8556396E-4 44.0 31 CGAACGT 20 7.8556396E-4 44.0 38 CGAACAC 20 7.8556396E-4 44.0 41 >>END_MODULE