FastQCFastQC Report
Fri 17 Jun 2016
SRR1527125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982233
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG95620.973496105302917No Hit
TAGAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT38300.38992784807678016No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC31840.32415933897557914No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG25490.2595107270881756No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23050.2346693707093938No Hit
TAGAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC23020.23436394419653994No Hit
TAGAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22530.22937531115325996No Hit
TAGAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG21110.2149184562115099No Hit
TAGAGAGGGATTTTTCTTTCTGTCTTCTCCCAGGTCCCTTACGACAGACTTT20730.21104972038202746No Hit
TAGAGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19610.19964713056881614No Hit
TAGAGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG19500.19852723335501862No Hit
TAGAGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC18390.18722645237942526No Hit
TAGAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA17560.17877631885713471No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG16920.1722605532495854No Hit
TAGAGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG16740.17042799417246213No Hit
TAGAGAGGGTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCT15270.1554620950426223No Hit
TAGAGAGGGAGAGTCCATACCGGGGAGAGAACGTACAACTACAAGAAGGGCA14850.15118612386266803No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG14700.14965899129839866No Hit
TAGAGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG14430.14691015268271376No Hit
TAGAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT14250.14507759360559053No Hit
TAGAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG13580.1382564014851873No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA13490.1373401219466257No Hit
TAGAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13050.13286053309143556No Hit
TAGAGAGGGGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCTC13050.13286053309143556No Hit
TAGAGAGAGTTAAATGGGAACTAGTATATGTGAAGAACTCTTTCTGTATTGC13040.13275872425381757No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12930.13163882704002006No Hit
TAGAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA12890.1312315916895482No Hit
TAGAGAGGGATGGGTAGTAAATACCAATGTAGTCAGTGTTCGTGTTGTGATA12370.12593753213341438No Hit
TAGAGAGGGGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCT12230.12451220840676296No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12220.124410399569145No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC11890.12105070792775238No Hit
TAGAGAGGGATTTTTGAGACTGTATTCCCCAGCATATATCTTCTAACCTTTG11750.11962538420110096No Hit
TAGAGAGGGGAATCCACAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG11630.11840367814968547No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG11160.11361866278164143No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10960.11158248602928225No Hit
TAGAGAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT10920.11117525067881043No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA10750.10944450043930513No Hit
TAGAGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA10630.10822279438788963No Hit
TAGAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA10480.10669566182362027No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC10430.10618661763553047No Hit
TAGAGAGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT10200.10384501437031744No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10150.10333597018222765No Hit
TAGAGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT9920.1009943669170146No Hit
TAGAGAGGGCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCA9870.1004853227289248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC1450.046.00000430
CTATGCG351.01936166E-746.00000421
ACGTTAT351.01936166E-746.00000432
ACTCTAT351.01936166E-746.00000417
TAGTTAT351.01936166E-746.00000436
CGTACAT351.01936166E-746.00000432
CGGTATG351.01936166E-746.00000437
TTCGCAT351.01936166E-746.00000426
AACACGC351.01936166E-746.00000417
TCACGAA206.311003E-446.041
CTTAATG206.311003E-446.015
AGCGTTA301.8611245E-646.016
TGTCACG253.41679E-546.011
AAATCGC253.41679E-546.043
CGAACAG206.311003E-446.011
ATCTATG900.046.011
TCCGATT301.8611245E-646.012
CTATCGG206.311003E-446.014
CGCATTC301.8611245E-646.030
TTATGTC301.8611245E-646.033
Produced by FastQC (version 0.11.5)