FastQCFastQC Report
Fri 17 Jun 2016
SRR1527124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956158
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG91300.9548631083984028No Hit
TAGAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT40000.418340901817482No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC30160.31542903997038146No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG25140.26292725679228746No Hit
TAGAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC23910.2500632740613999No Hit
TAGAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23730.2481807400032212No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22350.23374797889051813No Hit
TAGAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG20920.21879229165054312No Hit
TAGAGAGGGATTTTTCTTTCTGTCTTCTCCCAGGTCCCTTACGACAGACTTT20700.21649141669054695No Hit
TAGAGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG20260.21188966677055465No Hit
TAGAGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19970.20885669523237793No Hit
TAGAGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC18150.18982218419968247No Hit
TAGAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA17670.1848020933778727No Hit
TAGAGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG16880.1765398605669774No Hit
TAGAGAGGGTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCT15190.1588649574651888No Hit
TAGAGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG14510.1517531621342916No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG14400.15060272465429353No Hit
TAGAGAGGGAGAGTCCATACCGGGGAGAGAACGTACAACTACAAGAAGGGCA14130.14777892356702554No Hit
TAGAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13980.14621014518520997No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG13330.13941210553067587No Hit
TAGAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13180.13784332714886033No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA13020.1361699635415904No Hit
TAGAGAGAGTTAAATGGGAACTAGTATATGTGAAGAACTCTTTCTGTATTGC12930.13522869651250108No Hit
TAGAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG12900.13491494083613798No Hit
TAGAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA12080.1263389523488796No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC12070.12623436712342523No Hit
TAGAGAGGGGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCT12000.12550227054524463No Hit
TAGAGAGGGGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCTC11460.11985466837070861No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG11450.11975008314525425No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT11320.11839047521434742No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC11260.11776296386162119No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA10930.11431165142162696No Hit
TAGAGAGGGATTTTTGAGACTGTATTCCCCAGCATATATCTTCTAACCTTTG10610.1109649242070871No Hit
TAGAGAGGGATGGGTAGTAAATACCAATGTAGTCAGTGTTCGTGTTGTGATA10570.11054658330526965No Hit
TAGAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA10380.10855946402163659No Hit
TAGAGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10100.10563107770891421No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10040.10500356635618799No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCA9960.10416688455255303No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9890.10343478797437244No Hit
TAGAGAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT9830.10280727662164621No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG9700.10144766869073939No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC9600.1004018164361957No Hit
TAGAGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC9600.1004018164361957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG206.310918E-446.00000413
TGCCCTA206.310918E-446.00000435
GTACCAC206.310918E-446.00000412
CCGATTC206.310918E-446.00000437
CGAAACG206.310918E-446.00000427
CTATCGA206.310918E-446.00000411
GCTATTA206.310918E-446.00000430
TTATGAG405.6097633E-946.00000440
CGTCTAA206.310918E-446.00000446
CAATTAG206.310918E-446.00000440
TTATCGC405.6097633E-946.00000416
ACGGGTA206.310918E-446.00000443
TACATAT206.310918E-446.00000411
TCGGATA206.310918E-446.00000415
ACCTAAT206.310918E-446.00000423
TACGATG206.310918E-446.00000433
CTAAACA206.310918E-446.00000429
TCTCGAG206.310918E-446.00000423
GTCAATG206.310918E-446.00000424
ATAAGCG206.310918E-446.00000438
Produced by FastQC (version 0.11.5)