##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	956158
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25559792419244	33.0	31.0	34.0	31.0	34.0
2	32.458533003959595	34.0	31.0	34.0	31.0	34.0
3	32.85021094839974	34.0	31.0	34.0	31.0	34.0
4	35.99731006800131	37.0	35.0	37.0	35.0	37.0
5	36.223497580943736	37.0	37.0	37.0	35.0	37.0
6	35.772632765714455	37.0	35.0	37.0	35.0	37.0
7	36.46309919490294	37.0	37.0	37.0	35.0	37.0
8	36.65587800342621	37.0	37.0	37.0	35.0	37.0
9	38.65812867747799	39.0	39.0	39.0	39.0	39.0
10	38.09591301855969	39.0	39.0	39.0	37.0	39.0
11	37.699704442152864	39.0	38.0	39.0	35.0	39.0
12	37.39033507014531	39.0	37.0	39.0	35.0	39.0
13	37.270970906481985	39.0	37.0	39.0	35.0	39.0
14	38.18890810932921	40.0	38.0	40.0	35.0	40.0
15	38.23254629465005	40.0	38.0	40.0	35.0	40.0
16	38.29660474524085	40.0	38.0	40.0	35.0	40.0
17	38.267720397674864	40.0	38.0	40.0	35.0	40.0
18	38.211935684269754	40.0	38.0	40.0	34.0	40.0
19	38.177154821692646	40.0	38.0	40.0	34.0	40.0
20	38.143270254497686	40.0	38.0	40.0	34.0	40.0
21	33.832497348764534	40.0	35.0	40.0	10.0	40.0
22	34.56697219497196	40.0	34.0	40.0	16.0	40.0
23	36.584590622052005	39.0	34.0	40.0	30.0	40.0
24	37.33726643504525	39.0	36.0	40.0	33.0	40.0
25	37.58419424404753	39.0	37.0	40.0	33.0	40.0
26	37.58551515544502	40.0	37.0	40.0	33.0	40.0
27	37.59485043266908	40.0	37.0	40.0	33.0	40.0
28	37.4756692931503	40.0	36.0	40.0	33.0	40.0
29	37.418914028852974	40.0	36.0	40.0	33.0	40.0
30	37.26688267001897	40.0	36.0	40.0	33.0	40.0
31	37.148237006854515	40.0	36.0	40.0	33.0	40.0
32	37.0427439816432	39.0	35.0	40.0	32.0	40.0
33	36.723524773102355	39.0	35.0	40.0	31.0	40.0
34	36.5211649120752	39.0	35.0	40.0	31.0	40.0
35	36.446270386275074	39.0	35.0	40.0	31.0	40.0
36	36.2444930649537	38.0	35.0	40.0	31.0	40.0
37	36.007075190501986	38.0	35.0	40.0	30.0	40.0
38	35.909597576969496	38.0	35.0	40.0	30.0	40.0
39	35.67466464747458	38.0	35.0	40.0	29.0	40.0
40	30.80661982643036	36.0	29.0	40.0	8.0	40.0
41	31.633362896090397	36.0	28.0	40.0	12.0	40.0
42	33.35663771050391	35.0	30.0	40.0	23.0	40.0
43	34.104450310513535	36.0	33.0	39.0	26.0	40.0
44	34.86402561083001	37.0	34.0	40.0	28.0	40.0
45	35.07209373346246	37.0	34.0	40.0	29.0	40.0
46	35.0637248237216	36.0	34.0	40.0	29.0	40.0
47	34.95450228937059	36.0	34.0	40.0	29.0	40.0
48	34.77978430343102	36.0	34.0	40.0	28.0	40.0
49	34.555085038246816	35.0	34.0	39.0	28.0	40.0
50	34.42727770933256	35.0	34.0	39.0	28.0	40.0
51	34.19777589059549	35.0	34.0	39.0	27.0	40.0
52	33.77635181633161	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	8.0
16	45.0
17	68.0
18	185.0
19	355.0
20	669.0
21	1034.0
22	1716.0
23	2530.0
24	3693.0
25	5181.0
26	7361.0
27	10109.0
28	13063.0
29	15639.0
30	18811.0
31	24764.0
32	33615.0
33	44291.0
34	76981.0
35	100346.0
36	128189.0
37	153270.0
38	225087.0
39	89145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.60194863192066	0.3230637614285505	0.04172950495629383	0.03325810169448982
8	99.2619420639685	0.6202949721698715	0.040369897025387014	0.07739306683623418
9	98.42410982285355	1.0215884822382912	0.2418010412505046	0.3125006536576591
10	65.5218070653595	21.687419861570994	4.216562534643856	8.574210538425657
11	32.15629634432803	29.5221082708088	16.230894893940125	22.090700490923048
12	27.990666814480452	19.486110036207403	27.84100535685525	24.682217792456896
13	21.23048701156085	18.961510545328284	30.978143779584542	28.82985866352632
14	23.33651969653551	20.176163353755342	31.424408936598347	25.062908013110803
15	24.31041731596661	18.843852166692116	30.097013255131472	26.748717262209805
16	29.5759696619178	18.615228863848863	26.954750156354912	24.854051317878426
17	31.414577925405634	20.07534319641733	21.23477500580448	27.275303872372557
18	28.842513475806296	21.35327006624428	23.101307524488632	26.70290893346079
19	30.276063161109356	20.25198764220976	20.99527483951397	28.47667435716691
20	28.093892432003916	22.097289360126673	21.557525011556667	28.251293196312744
21	34.58999454065123	19.653028056032582	23.024332798554216	22.732644604761976
22	32.65705040380355	18.86738384241935	22.203129608286496	26.272436145490595
23	30.529159406708935	22.559137715733176	21.494041779705864	25.41766109785203
24	29.886901537193644	20.976449498932183	22.165060586221106	26.971588377653067
25	29.91022404246997	20.640312584321837	22.3025901576936	27.14687321551459
26	29.304989342765523	21.09912796839016	21.43484654209869	28.161036146745623
27	28.38756774507979	20.15765176884992	23.46756498402984	27.987215502040456
28	30.971764080831832	21.46120201891319	20.82291838796517	26.744115512289813
29	31.716201715616037	21.8095754049017	20.728582514605325	25.745640364876937
30	28.928273360678887	22.530167608282316	20.947793147157686	27.593765883881115
31	31.67154382434702	22.941710470445262	19.090568713538975	26.29617699166874
32	32.29476718282962	22.742475615954685	20.482493479111195	24.480263722104507
33	33.63711855153646	21.212393767557245	20.90198481840867	24.24850286249762
34	29.6561865298413	23.178177665197595	23.51002658556431	23.655609219396794
35	30.694822403828653	24.91157319187833	22.711309218769284	21.68229518552373
36	31.34868923336938	24.62249962872245	21.172651381884585	22.856159756023587
37	30.59504809874519	25.9350442081748	19.13805040589526	24.331857287184754
38	33.8653235134779	24.3306022644793	18.755268480732266	23.048805741310535
39	30.352201205240135	25.208281476492377	19.26606272185141	25.17345459641607
40	28.775578931515504	28.929423798158883	20.49629872887117	21.798698541454446
41	28.013257223178595	24.283120572123018	23.139690302230385	24.563931902468003
42	27.665929689444628	23.947401998414488	21.82223021718168	26.564438094959204
43	28.442579573668787	21.88906017624702	24.60764852670793	25.06071172337626
44	26.941677003173115	22.891718732678072	23.230156522248414	26.9364477419004
45	27.695945649150033	23.693678241462184	22.845910403929057	25.764465705458722
46	27.636854996768317	24.858862238249326	22.65859826514028	24.845684499842076
47	29.199253679831155	23.04148477552873	22.86536325586357	24.89389828877654
48	29.920264224113584	23.763541172065704	21.134896115495554	25.181298488325147
49	27.76256643776447	24.69476801951142	23.379190468520893	24.163475074203216
50	24.874654607292936	25.976669127905637	24.240972726264907	24.90770353853652
51	25.99057896289107	24.805000847140327	21.86029923924707	27.344120950721535
52	27.20627762357267	25.704747541724277	21.974401720217788	25.11457311448526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	21.0
6	38.0
7	70.0
8	102.0
9	1435.0
10	2768.0
11	2507.0
12	2246.0
13	1879.0
14	1540.0
15	1568.0
16	1495.0
17	1422.0
18	1691.0
19	1960.0
20	2017.0
21	2074.0
22	2428.5
23	2783.0
24	3655.0
25	4527.0
26	4931.5
27	5336.0
28	6795.0
29	8254.0
30	8418.5
31	8583.0
32	9585.5
33	10588.0
34	11769.5
35	12951.0
36	13093.0
37	13235.0
38	16091.5
39	20938.5
40	22929.0
41	22310.5
42	21692.0
43	25243.5
44	28795.0
45	28040.0
46	27285.0
47	28368.5
48	29452.0
49	32520.5
50	35589.0
51	42564.0
52	49539.0
53	53096.0
54	56653.0
55	65954.0
56	75255.0
57	87175.5
58	99096.0
59	88044.5
60	76993.0
61	79160.0
62	81327.0
63	74145.0
64	61843.5
65	56724.0
66	47963.5
67	39203.0
68	32848.5
69	26494.0
70	26764.0
71	27034.0
72	23818.0
73	20602.0
74	15174.0
75	9746.0
76	6869.0
77	3992.0
78	2620.5
79	1249.0
80	840.0
81	431.0
82	279.5
83	128.0
84	96.5
85	65.0
86	41.0
87	17.0
88	10.5
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	956158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.973627376069222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.62336239532729	9.72890922781334
2	10.340200183234758	2.8898020870897043
3	4.986713468676001	2.0904742752751226
4	2.894687327475515	1.6179712833749007
5	1.7919064550076704	1.2519716547525168
6	1.2611259852842873	1.0573502755584476
7	0.9047733959657254	0.8850076406504044
8	0.6148817097130337	0.6873702313512238
9	0.48921636634177396	0.615251448858107
>10	3.849280094916797	12.276963220881312
>50	1.6765009294485655	16.938947474613705
>100	1.471551635497242	36.08506154216694
>500	0.06674593864315133	6.490485266664995
>1k	0.028268868131217033	6.382623235302352
>5k	7.852463369782508E-4	1.0018111356469275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9130	0.9548631083984028	No Hit
TAGAGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4000	0.418340901817482	No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3016	0.31542903997038146	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG	2514	0.26292725679228746	No Hit
TAGAGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2391	0.2500632740613999	No Hit
TAGAGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2373	0.2481807400032212	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2235	0.23374797889051813	No Hit
TAGAGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2092	0.21879229165054312	No Hit
TAGAGAGGGATTTTTCTTTCTGTCTTCTCCCAGGTCCCTTACGACAGACTTT	2070	0.21649141669054695	No Hit
TAGAGAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	2026	0.21188966677055465	No Hit
TAGAGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1997	0.20885669523237793	No Hit
TAGAGAGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	1815	0.18982218419968247	No Hit
TAGAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1767	0.1848020933778727	No Hit
TAGAGAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	1688	0.1765398605669774	No Hit
TAGAGAGGGTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCT	1519	0.1588649574651888	No Hit
TAGAGAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG	1451	0.1517531621342916	No Hit
TAGAGAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1440	0.15060272465429353	No Hit
TAGAGAGGGAGAGTCCATACCGGGGAGAGAACGTACAACTACAAGAAGGGCA	1413	0.14777892356702554	No Hit
TAGAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1398	0.14621014518520997	No Hit
TAGAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG	1333	0.13941210553067587	No Hit
TAGAGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1318	0.13784332714886033	No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1302	0.1361699635415904	No Hit
TAGAGAGAGTTAAATGGGAACTAGTATATGTGAAGAACTCTTTCTGTATTGC	1293	0.13522869651250108	No Hit
TAGAGAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1290	0.13491494083613798	No Hit
TAGAGAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1208	0.1263389523488796	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1207	0.12623436712342523	No Hit
TAGAGAGGGGTCGTAGAAACCTCGACATTGAGCGCCCAACCTACACTAACCT	1200	0.12550227054524463	No Hit
TAGAGAGGGGTCAAGGTCTGAGATTGACAAGGGGCTCCCTTCCCCATCTCTC	1146	0.11985466837070861	No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1145	0.11975008314525425	No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1132	0.11839047521434742	No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1126	0.11776296386162119	No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	1093	0.11431165142162696	No Hit
TAGAGAGGGATTTTTGAGACTGTATTCCCCAGCATATATCTTCTAACCTTTG	1061	0.1109649242070871	No Hit
TAGAGAGGGATGGGTAGTAAATACCAATGTAGTCAGTGTTCGTGTTGTGATA	1057	0.11054658330526965	No Hit
TAGAGAGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA	1038	0.10855946402163659	No Hit
TAGAGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1010	0.10563107770891421	No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1004	0.10500356635618799	No Hit
TAGAGAGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCA	996	0.10416688455255303	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	989	0.10343478797437244	No Hit
TAGAGAGGGGTCCGTGGTCGTTCCGCGCCGTCATGTCTGTCCTGACGCCACT	983	0.10280727662164621	No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG	970	0.10144766869073939	No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	960	0.1004018164361957	No Hit
TAGAGAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC	960	0.1004018164361957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.017988658778151728	0.0	0.0	0.0	0.0
5	0.017988658778151728	0.0	0.0	0.0	0.0
6	0.06787581131988646	0.0	0.0	0.0	0.0
7	0.06808498177079521	0.0	0.0	0.0	0.0
8	0.07080419763260884	0.0	0.0	0.0	0.0
9	0.07540594755260113	0.0	0.0	0.0	0.0
10	0.11148785033435897	0.0	0.0	0.0	0.0
11	0.1635712926106355	0.0	0.0	0.0	0.0
12	0.1904496955524087	0.0	0.0	0.0	0.0
13	0.20791542820328857	0.0	0.0	0.0	0.0
14	0.2340617345668812	0.0	0.0	0.0	0.0
15	0.2546650239813922	0.0	0.0	0.0	0.0
16	0.2758958247486294	0.0	0.0	0.0	0.0
17	0.28991024495951506	0.0	0.0	0.0	0.0
18	0.30120544930858706	0.0	0.0	0.0	0.0
19	0.31793908538128635	0.0	0.0	0.0	0.0
20	0.3316397499158089	0.0	0.0	0.0	0.0
21	0.3448174883230596	0.0	0.0	0.0	0.0
22	0.3624923914248482	0.0	0.0	0.0	0.0
23	0.3781801752430038	0.0	0.0	0.0	0.0
24	0.3967963453738817	0.0	0.0	0.0	0.0
25	0.4139483223483985	0.0	0.0	0.0	0.0
26	0.43538829356654446	0.0	0.0	0.0	0.0
27	0.4531677818937874	0.0	0.0	0.0	0.0
28	0.49291016756644823	0.0	0.0	0.0	0.0
29	0.5097483888646018	0.0	0.0	0.0	0.0
30	0.5319204566609285	0.0	0.0	0.0	0.0
31	0.5479219961554471	0.0	0.0	0.0	0.0
32	0.5696757230499562	0.0	0.0	0.0	0.0
33	0.5862001886717467	0.0	0.0	0.0	0.0
34	0.6024108986171741	0.0	0.0	0.0	0.0
35	0.6408982615843825	0.0	0.0	0.0	0.0
36	0.6601419430679867	0.0	0.0	0.0	0.0
37	0.6765618234643228	0.0	0.0	0.0	0.0
38	0.6943413117915658	0.0	0.0	0.0	0.0
39	0.7100290956097214	0.0	0.0	0.0	0.0
40	0.7306323850242324	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	6.310918E-4	46.000004	13
TGCCCTA	20	6.310918E-4	46.000004	35
GTACCAC	20	6.310918E-4	46.000004	12
CCGATTC	20	6.310918E-4	46.000004	37
CGAAACG	20	6.310918E-4	46.000004	27
CTATCGA	20	6.310918E-4	46.000004	11
GCTATTA	20	6.310918E-4	46.000004	30
TTATGAG	40	5.6097633E-9	46.000004	40
CGTCTAA	20	6.310918E-4	46.000004	46
CAATTAG	20	6.310918E-4	46.000004	40
TTATCGC	40	5.6097633E-9	46.000004	16
ACGGGTA	20	6.310918E-4	46.000004	43
TACATAT	20	6.310918E-4	46.000004	11
TCGGATA	20	6.310918E-4	46.000004	15
ACCTAAT	20	6.310918E-4	46.000004	23
TACGATG	20	6.310918E-4	46.000004	33
CTAAACA	20	6.310918E-4	46.000004	29
TCTCGAG	20	6.310918E-4	46.000004	23
GTCAATG	20	6.310918E-4	46.000004	24
ATAAGCG	20	6.310918E-4	46.000004	38
>>END_MODULE