FastQCFastQC Report
Fri 17 Jun 2016
SRR1527122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210645
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGATGGAAATGAGATTGAAGCTAGCTTGCATTCTCCCCTCATTCAT12340.5858197441192527No Hit
CCAGACGGGATCTTCTCATGGGTTTTCTTAATATTTTAAAACTACTTGTTTA10850.515084621044886No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG10700.5079636355004866No Hit
CCAGACGGGGCTCCAGACGGGGTTCATAAGAATATCAACAGACAGCTAGAGC9020.42820859740321393No Hit
CCAGACGGGATTGCCTCTCTGCTCCTGACCCTCGGACTATGGGGCGTCCTCC8930.42393600607657433No Hit
CCAGACGGGACCTGTAAATACTGTATCCTCAGTTTAGAAAATTAGTGCAATG7570.3593724038073536No Hit
CCAGACGGGGCTGACGGAGAAAGCTTCCTCGGTTATGTGGACATGCTTGGTG6640.31522229343207764No Hit
CCAGACGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC6630.31474756106245105No Hit
CCAGACGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG6580.3123738992143179No Hit
CCAGACGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC6450.3062023784091718No Hit
CCAGACGGGGCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGTC6410.30430344893066535No Hit
CCAGACGGGAGTATACGCTCTAGTTATCATGTGTAACGTGGGTCTGTTTAGC6210.2948088015381329No Hit
CCAGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC6130.29101094258111987No Hit
CCAGACGGGATTCATGTTTGGGCCCTGAAGGGCCTCAGGGGAAAACAAGCCA5720.27154691542642834No Hit
CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5610.2663248593605355No Hit
CCAGACGGGGAGTGTCCATGGCCTGTTTGTCTTCAGTGTCTCCCTCATCTTC5600.2658501269909089No Hit
CCAGACGGGTTATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACT5570.264425929882029No Hit
CCAGACGGGGCTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA5400.2563554795983764No Hit
CCAGACGGGATTCCTTTCCTTCCCTAAACATCCTCTTCCTCCCCATCACACA5380.2554060148591232No Hit
CCAGACGGGAGTGTATGTGAATGTTTTGGCGGTGGCCAGGATCCCAGGCAAG5310.2520828882717368No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCAGACGGGAAG5310.2520828882717368No Hit
CCAGACGGGACCCACTCAGCCTTCTGTCTCTGCCCCTTGGCCGCTCACACCC5160.24496190272733748No Hit
CCAGACGGGGCTGGCTCCCGCTCTCACAGCCATTGCAGTACATTGAGCTCCA5090.2416387761399511No Hit
CCAGACGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT5020.23831564955256473No Hit
CCAGACGGGGTATGGGAGGGTGACCAAGGCTGTCGCAGTTGCCTACAACACA5000.2373661848133115No Hit
CCAGACGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA4940.23451779059555175No Hit
CCAGACGGAGCTCCAGACGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCC4650.22075055187637968No Hit
CCAGACGGGGGAAAGCTGACTCCCCTTCTATGTCCCCTACTGTCCGAGGTCC4520.2145790310712336No Hit
CCAGACGGGGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCGGC4480.2126801015927271No Hit
CCAGACGGGAGTTCCGCTGGCCTGGCGCGCCATCAGAGCCACTCAGAGCGCC4430.21030643974459398No Hit
CCAGACGGCCTGTACATTGTTGCTTGAGAGTATGTACATTTTAGACCAGATT4410.20935697500534073No Hit
CCAGACGGGAGGCTGACTACCTGTACCGGTGACCAGACCACTGGTGACCTTG4330.20555911604832774No Hit
CCAGACGGGATGTAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGACA4240.20128652472168815No Hit
CCAGACGGGGTGAGCCACCATGTGGCTCACAACCATCCGTAATGAGATCTGA4160.19748866576467516No Hit
CCAGACGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT3940.18704455363288947No Hit
CCAGACGGGAGATTAATTTTTATAAATGAAATATTTTGATGGTCTAAGAAAA3930.18656982126326283No Hit
CCAGACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT3760.17849937097961024No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC3620.17185311780483753No Hit
CCAGACGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG3600.17090365306558428No Hit
CCAGACGGGGCCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA3580.16995418832633102No Hit
CCAGACGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAAC3580.16995418832633102No Hit
CCAGACGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3530.1675805264781979No Hit
CCAGACGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGCTT3500.16615632936931804No Hit
CCAGACGGGGGCTGTTCATGCTCTGAGTTTAGGTATTGAGACATGAATACTA3460.16425739989081156No Hit
CCAGACGGGCGGGCCCACGGAAACAGCGGTATGGTTCGTGCCAAATTCCGAA3450.16378266752118492No Hit
CCAGACGGGGGAAGGTTGAGGCGCAATGGCAGGTGTTGGGGTCGGACCGCTG3420.16235847041230506No Hit
CCAGACGGGATATTAGGCACACTTCGATGACCCCCGGCTCTCAGAACCAGCC3400.16140900567305183No Hit
CCAGACGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG3380.16045954093379858No Hit
CCAGACGGGGTCCCTCCTCTCTCCGCGCCGGCTGGTGGTCGTTCTATTCTCT3370.15998480856417194No Hit
CCAGACGGGCAGAGTGCAGTGCTCCAGACGGGTCTATTCACAAGCCTGCCTA3360.15951007619454532No Hit
CCAGACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC3360.15951007619454532No Hit
CCAGACGGGACTGTTTGAACCAAGACATGAGTTGATAATGAACACTTGCTCT3350.1590353438249187No Hit
CCAGACGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG3320.15761114671603882No Hit
CCAGACGGGGCTCCAGACGGGCGATCGCTGGCCGCTTCACACCTGGGACCTT3320.15761114671603882No Hit
CCAGACGGGATTTAATGCTGGGGAAACTTGCCCCTCTGACTAGATGCTCAGA3290.15618694960715895No Hit
CCAGACGGGGCTCCAGACGGGATGCAGTGCTCCAGACGGGGTTTTATCCAAC3180.1509648935412661No Hit
CCAGACGGGGTGCTCCAGACGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGG3170.1504901611716395No Hit
CCAGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC3120.14811649932350637No Hit
CCAGACGGGATTGCTGTGAATTATATTGACAGTTGGTGAGCTGTCTTATATT3070.14574283747537325No Hit
CCAGACGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA3050.14479337273612No Hit
CCAGACGGGGCCGCGTCCCTCCTCTCTCCGCGCCGGCTGGTGGTCGTTCTAT3030.14384390799686678No Hit
CCAGACGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG3020.14336917562724014No Hit
CCAGACGGGCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGCACAAAAT3000.14241971088798688No Hit
CCAGACGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA3000.14241971088798688No Hit
CCAGACGGGGCTCCAGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATC3000.14241971088798688No Hit
CCAGACGGGCTCTCTGGCCTTTGCTAACCTGCCTACCTCTTTACCCGCCCCC2980.14147024614873366No Hit
CCAGACGGGTGAGGTAAGTTCTTTAACGTTTCATTAAAAAACATTTTTTACA2970.14099551377910702No Hit
CCAGACGGGGGTGGATCTTAGAGCCCTAGAGGGAGGCTTTTGCTTGTAGCCC2970.14099551377910702No Hit
CCAGACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG2960.1405207814094804No Hit
CCAGACGGGGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCCCGGAAGAAT2940.13957131667022715No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC2930.13909658430060054No Hit
CCAGACGGGCTCTGGTCAAATCAGTCTGGACTGTATAAGAGCAAGCTGAGCC2930.13909658430060054No Hit
CCAGACGGGTGGCATACGCGAAGCTGCCAAAAGCCTTAGACAAGCGCCAAGC2920.13862185193097393No Hit
CCAGACGGGCATTGCCACCATCAAGACCAAGCGTACCATCCAGTTTGTGGAC2900.13767238719172067No Hit
CCAGACGGGCTGCCAATGAAGGGGACACAGGTTCAATCCCTGGTCCAGGAAG2890.13719765482209403No Hit
CCAGACGGGTGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA2860.13577345771321417No Hit
CCAGACGGGGTCCAGCTGTCTCTGTCTGGGAATGATCTAACCTGGTTTGAGC2850.13529872534358756No Hit
CCAGACGGGGCAGAGTGCAGAGCTCCAGACGGGCTTCCTTTTGTGACTGGCG2850.13529872534358756No Hit
CCAGACGGGGCTCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG2850.13529872534358756No Hit
CCAGACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAG2840.13482399297396092No Hit
CCAGACGGGTTTCGGTTTTGTTGTTTTTGTATTTTGTGTATCTTTTTGTATG2830.1343492606043343No Hit
CCAGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA2830.1343492606043343No Hit
CCAGACGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATG2830.1343492606043343No Hit
CCAGACGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG2800.13292506349545444No Hit
CCAGACGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA2800.13292506349545444No Hit
CCAGACGGGGGGAGGCTGGAGCTATGTTCCCAACACTAATTTTCTTATACAA2790.13245033112582782No Hit
CCAGACGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG2770.13150086638657457No Hit
CCAGACGGGAACTGACCTGTTTCTAAATGAAAGAGATGTGGGAGAGTGCTCT2770.13150086638657457No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGA2770.13150086638657457No Hit
CCAGACGGGGGGCGCTGGTCCCATCCATTCCCGATGCCTTTCCACCTAATGA2760.13102613401694796No Hit
CCAGACGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2730.12960193690806807No Hit
CCAGACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA2710.12865247216881484No Hit
CCAGACGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGG2710.12865247216881484No Hit
CCAGACGGGGATCTGACCATTCCGTTGGCTTGGAATGGTCAGATCTCACAGG2700.1281777397991882No Hit
CCAGACGGGATTTTTCTTCCTGTTCAGTGCTTTATCACCATCTGTTGTTTGT2670.12675354269030834No Hit
CCAGACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG2670.12675354269030834No Hit
CCAGACGGGAAGAACAACCTGAAGGACTGTGGCCTCTTCTGAGGGGCAGTGG2670.12675354269030834No Hit
CCAGACGGGAGGGTGTCACGGCTGCTGTGCAATTATCTGAAGTTGCCTGACA2660.12627881032068172No Hit
CCAGACGGGGAGGCGTGGAGGTTGGTGGCTGTTGAGCGGTTCCCGTGGTAAA2650.12580407795105508No Hit
CCAGACGGGGCCTCCTCTCTCCGTTCGGACCATGGCCAACCTCGAGCGTACC2650.12580407795105508No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2630.12485461321180184No Hit
CCAGACGGCAACTGTACGGGAGCTTAACTTTATAAGGAAATGTATTTTGACA2610.1239051484725486No Hit
CCAGACGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG2600.12343041610292198No Hit
CCAGACGGGGAGTGCAGTGCTCCAGACGGGGTGTGTGCGGTTGTGTGTGTAT2600.12343041610292198No Hit
CCAGACGGGTGCTAAGGTTGAGCGAGCTGACGGATATGAACCGCCAGTCCAA2590.12295568373329535No Hit
CCAGACGGGAACAGTGTTGGATAAAGCCCCCCACATCTGCATGGCTCCTCCC2580.12248095136366874No Hit
CCAGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC2570.12200621899404211No Hit
CCAGACGGGGCTCGTTCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGC2560.12153148662441549No Hit
CCAGACGGGGAAAGTCTGGCGGTAGAGTGTCTGGGTGCTGGCTGACGTGCGG2550.12105675425478886No Hit
CCAGACGGGATATGATTTTCCACTAATAAGCAAGCAATTAATTTTTTCTGAA2550.12105675425478886No Hit
CCAGACGGGTCCACCACCCCGGCTGTGCTCTCCGACTGCCGCCGCCGCCGCC2540.12058202188516223No Hit
CCAGACGGGATTTTCTCCTTCCTTTTCCTCCCTTCTGCTCCACCTTCCCTTT2540.12058202188516223No Hit
CCAGACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2510.11915782477628237No Hit
CCAGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA2470.11725889529777588No Hit
CCAGACGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGC2440.11583469818889601No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA2430.11535996581926938No Hit
CCAGACGGGGCTCACATGGGTTATATGCTAAGAAGTATCCTGCATGTCATTG2420.11488523344964277No Hit
CCAGACGGGGACGGGATGTTCCATGCAAACTTCCGTTACTGTTGGCAAACTC2410.11441050108001614No Hit
CCAGACGGGCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAA2390.11346103634076289No Hit
CCAGACGGGGCTCCAGACGGGGGGCTGGTGAGATGGCTCAAAGGGTAAAAGC2380.11298630397113626No Hit
CCAGACGGGCTTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGA2380.11298630397113626No Hit
CCAGACGGGAACAAAAATTGAAGCAAATACATAGGATTGCAGGGTAAAGAAC2350.1115621068622564No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT2350.1115621068622564No Hit
CCAGACGGGTACTCGGAAGGGTTGAGCGTCCACACTCACATCGTCAGCACCA2340.11108737449262979No Hit
CCAGACGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC2330.11061264212300316No Hit
CCAGACGGGAGCTTGTACATGAAACTTTGTATAAAAAAACAAAAACAAAACA2310.10966317738374991No Hit
CCAGACGGGGATAAGAGATTTTCTCACAGGAAATTGTCTTTTTCATAATTCT2310.10966317738374991No Hit
CCAGACGGGTGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCT2300.10918844501412328No Hit
CCAGACGGGGATGAGGACGAGGAGGAGAGTGAAAGCTCAGACTCTGAGGAGG2300.10918844501412328No Hit
CCAGACGGGGCTCCAGACGGTGTGTGTGGTTGCCACACAGCATTGTACCCAG2280.10823898027487004No Hit
CCAGACGGGGCTCCTGCAGGTGTAGCCCTACCTACAGTGTGACTAGGCCGGT2280.10823898027487004No Hit
CCAGACGGGGCTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCA2280.10823898027487004No Hit
CCAGACGGGCCTTCTCCCGGCTGAGGCTGCTCAAGAGCTGCGGTTGGGTCAC2270.10776424790524342No Hit
CCAGACGGGGCTCCAGACGGGTAGCATTTTTTTTGTCTTTGTAATTTACAGA2270.10776424790524342No Hit
CCAGACGGAGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGCGGCGCGGGAAG2270.10776424790524342No Hit
CCAGACGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA2240.10634005079636355No Hit
CCAGACGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACCT2240.10634005079636355No Hit
CCAGACGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACCA2240.10634005079636355No Hit
CCAGACGGGAGCACCTTGGCCACCACTTCTCTGTGCCACCGTGTGTTTGGCA2230.10586531842673692No Hit
CCAGACGGGAGATCATGAAGGTGGAAGAGATTGCAGCTGGCAAGTGCCGCCG2230.10586531842673692No Hit
CCAGACGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGG2220.1053905860571103No Hit
CCAGACGGGAGCATCCCCCACCTCCAGATCGACAAATACCTGGAAGACAAGG2210.10491585368748368No Hit
CCAGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC2180.10349165657860382No Hit
CCAGACGGGGTGATCATCTAGGACCAGGGCGACCGGGGCGCGCGATCCTTTC2180.10349165657860382No Hit
CCAGACGGGGAGACATTGAAAGTGTATTAACCAGCATGAAAGATGTGTTTCG2150.10206745946972394No Hit
CCAGACGGGGGCTAAAACAGAGAGGCTGTGAACAAGGACATTACCGAGCAGG2150.10206745946972394No Hit
CCAGACGGCCCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACG2140.10159272710009731No Hit
CCAGACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT2130.1011179947304707No Hit
CCAGACGGGCTTTCCTTGGCGTGATTCCGTCCTGCGCGTCTGTTCTGTGGAA2130.1011179947304707No Hit
CCAGACGGGAGAGTCTGACCCCAGGCTGGGACAGGATTCCAGGCCCTCTTCC2120.10064326236084407No Hit
CCAGACGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAAGCC2120.10064326236084407No Hit
CCAGACGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGTT2110.10016852999121745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACAAG206.29966E-446.00000425
CCCTGCA206.29966E-446.00000446
CCTTCGT206.29966E-446.00000433
CCTTCGG206.29966E-446.00000417
CTTAATT206.29966E-446.00000441
ATTTAGG206.29966E-446.00000410
TTGATGC206.29966E-446.00000437
CATGGTT206.29966E-446.00000428
CATGGTC206.29966E-446.00000432
CTAGATG206.29966E-446.00000440
GTTGAAC206.29966E-446.00000445
GCCCCAA206.29966E-446.00000411
CGTGGTA351.0142503E-746.00000444
TATCAGA206.29966E-446.00000428
CAGGTAT206.29966E-446.00000412
AACGTCA206.29966E-446.00000417
ACTATTA206.29966E-446.00000424
TTAGGGT206.29966E-446.00000426
CTATGGG1400.046.00000437
AGCGTCC351.0142503E-746.00000425
Produced by FastQC (version 0.11.5)