FastQCFastQC Report
Fri 17 Jun 2016
SRR1527121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204641
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGATGGAAATGAGATTGAAGCTAGCTTGCATTCTCCCCTCATTCAT11100.5424132993877082No Hit
CCAGACGGGATCTTCTCATGGGTTTTCTTAATATTTTAAAACTACTTGTTTA11080.5414359781275502No Hit
CCAGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG10180.4974565214204387No Hit
CCAGACGGGGCTCCAGACGGGGTTCATAAGAATATCAACAGACAGCTAGAGC9740.47595545369696196No Hit
CCAGACGGGATTGCCTCTCTGCTCCTGACCCTCGGACTATGGGGCGTCCTCC9730.475466793066883No Hit
CCAGACGGGGCTGACGGAGAAAGCTTCCTCGGTTATGTGGACATGCTTGGTG6890.3366871741244423No Hit
CCAGACGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAGG6520.3186067308115187No Hit
CCAGACGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC6460.31567476703104463No Hit
CCAGACGGGAGTATACGCTCTAGTTATCATGTGTAACGTGGGTCTGTTTAGC6440.3146974457708866No Hit
CCAGACGGGGCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGTC6400.3127428032505705No Hit
CCAGACGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC6280.3068788756896223No Hit
CCAGACGGGACCTGTAAATACTGTATCCTCAGTTTAGAAAATTAGTGCAATG6240.3049242331693063No Hit
CCAGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC6170.30150360875875315No Hit
CCAGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5990.2927077174173308No Hit
CCAGACGGGGAGTGTCCATGGCCTGTTTGTCTTCAGTGTCTCCCTCATCTTC5800.2834231654458295No Hit
CCAGACGGGATTCCTTTCCTTCCCTAAACATCCTCTTCCTCCCCATCACACA5780.2824458441856715No Hit
CCAGACGGGACCCACTCAGCCTTCTGTCTCTGCCCCTTGGCCGCTCACACCC5450.2663200433930639No Hit
CCAGACGGGATTCATGTTTGGGCCCTGAAGGGCCTCAGGGGAAAACAAGCCA5300.2589901339418787No Hit
CCAGACGGGGCTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA5210.25459218827116753No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCAGACGGGAAG5180.2531262063809305No Hit
CCAGACGGGAGTGTATGTGAATGTTTTGGCGGTGGCCAGGATCCCAGGCAAG5010.2448189756695872No Hit
CCAGACGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACGT4950.24188701188911313No Hit
CCAGACGGAGCTCCAGACGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCC4790.23406844180784886No Hit
CCAGACGGGGCTGGCTCCCGCTCTCACAGCCATTGCAGTACATTGAGCTCCA4620.22576121109650557No Hit
CCAGACGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA4580.22380656857618952No Hit
CCAGACGGGGTATGGGAGGGTGACCAAGGCTGTCGCAGTTGCCTACAACACA4580.22380656857618952No Hit
CCAGACGGGTTATTTACAGTTGGTGGTCTAACCGGAATTGTTTTATCCAACT4380.21403335597460918No Hit
CCAGACGGGGGAAAGCTGACTCCCCTTCTATGTCCCCTACTGTCCGAGGTCC4360.21305603471445117No Hit
CCAGACGGGAGATTAATTTTTATAAATGAAATATTTTGATGGTCTAAGAAAA4200.20523746463318693No Hit
CCAGACGGGAGGCTGACTACCTGTACCGGTGACCAGACCACTGGTGACCTTG4050.19790755518200165No Hit
CCAGACGGGATGTAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGACA4000.19546425203160658No Hit
CCAGACGGGGGGTTCGAACAAAGGCGCCATCATCGGACTCATGGTGGGCGGC4000.19546425203160658No Hit
CCAGACGGGGCCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGA3970.19399827014136953No Hit
CCAGACGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG3970.19399827014136953No Hit
CCAGACGGCCTGTACATTGTTGCTTGAGAGTATGTACATTTTAGACCAGATT3910.19106630636089542No Hit
CCAGACGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT3800.18569103943002624No Hit
CCAGACGGGACTGTTTGAACCAAGACATGAGTTGATAATGAACACTTGCTCT3770.1842250575397892No Hit
CCAGACGGGGTGAGCCACCATGTGGCTCACAACCATCCGTAATGAGATCTGA3700.18080443312923608No Hit
CCAGACGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCG3640.17787246934876197No Hit
CCAGACGGGAGTTCCGCTGGCCTGGCGCGCCATCAGAGCCACTCAGAGCGCC3640.17787246934876197No Hit
CCAGACGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT3600.17591782682844592No Hit
CCAGACGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGCTT3580.17494050556828789No Hit
CCAGACGGGCAGAGTGCAGTGCTCCAGACGGGTCTATTCACAAGCCTGCCTA3550.17347452367805083No Hit
CCAGACGGGGGAAGGTTGAGGCGCAATGGCAGGTGTTGGGGTCGGACCGCTG3530.1724972024178928No Hit
CCAGACGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG3490.17054255989757675No Hit
CCAGACGGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAAC3470.1695652386374187No Hit
CCAGACGGGGGCTGTTCATGCTCTGAGTTTAGGTATTGAGACATGAATACTA3400.16614461422686558No Hit
CCAGACGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG3350.1637013110764705No Hit
CCAGACGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA3340.1632126504463915No Hit
CCAGACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC3320.16223532918623343No Hit
CCAGACGGGGTCCCTCCTCTCTCCGCGCCGGCTGGTGGTCGTTCTATTCTCT3310.16174666855615444No Hit
CCAGACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC3310.16174666855615444No Hit
CCAGACGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAG3310.16174666855615444No Hit
CCAGACGGGCGGGCCCACGGAAACAGCGGTATGGTTCGTGCCAAATTCCGAA3210.15686006225536428No Hit
CCAGACGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA3190.15588274099520624No Hit
CCAGACGGGCTCTGGTCAAATCAGTCTGGACTGTATAAGAGCAAGCTGAGCC3170.1549054197350482No Hit
CCAGACGGGCATTGCCACCATCAAGACCAAGCGTACCATCCAGTTTGTGGAC3150.15392809847489017No Hit
CCAGACGGGATATTAGGCACACTTCGATGACCCCCGGCTCTCAGAACCAGCC3150.15392809847489017No Hit
CCAGACGGGGCTCCAGACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATC3130.15295077721473213No Hit
CCAGACGGGGGTGGATCTTAGAGCCCTAGAGGGAGGCTTTTGCTTGTAGCCC3070.15001881343425805No Hit
CCAGACGGGGCAGAGTGCAGAGCTCCAGACGGGCTTCCTTTTGTGACTGGCG3020.14757551028386295No Hit
CCAGACGGGATTTAATGCTGGGGAAACTTGCCCCTCTGACTAGATGCTCAGA3000.14659818902370494No Hit
CCAGACGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG2940.14366622524323083No Hit
CCAGACGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA2900.14171158272291476No Hit
CCAGACGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA2880.14073426146275675No Hit
CCAGACGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA2860.13975694020259868No Hit
CCAGACGGGTGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAA2840.13877961894244067No Hit
CCAGACGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAG2830.13829095831236166No Hit
CCAGACGGGGGGAGGCTGGAGCTATGTTCCCAACACTAATTTTCTTATACAA2820.13780229768228264No Hit
CCAGACGGGGATCTGACCATTCCGTTGGCTTGGAATGGTCAGATCTCACAGG2810.13731363705220362No Hit
CCAGACGGGTTTCGGTTTTGTTGTTTTTGTATTTTGTGTATCTTTTTGTATG2810.13731363705220362No Hit
CCAGACGGGCTCTCTGGCCTTTGCTAACCTGCCTACCTCTTTACCCGCCCCC2790.13633631579204558No Hit
CCAGACGGGGCTCCAGACGGGCGATCGCTGGCCGCTTCACACCTGGGACCTT2790.13633631579204558No Hit
CCAGACGGGCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGCACAAAAT2780.13584765516196656No Hit
CCAGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC2780.13584765516196656No Hit
CCAGACGGGGTCCAGCTGTCTCTGTCTGGGAATGATCTAACCTGGTTTGAGC2770.13535899453188754No Hit
CCAGACGGGGCCTCCTCTCTCCGTTCGGACCATGGCCAACCTCGAGCGTACC2770.13535899453188754No Hit
CCAGACGGGGCCGCGTCCCTCCTCTCTCCGCGCCGGCTGGTGGTCGTTCTAT2760.13487033390180853No Hit
CCAGACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC2750.1343816732717295No Hit
CCAGACGGGCTGCCAATGAAGGGGACACAGGTTCAATCCCTGGTCCAGGAAG2730.1334043520115715No Hit
CCAGACGGGGGGCGCTGGTCCCATCCATTCCCGATGCCTTTCCACCTAATGA2730.1334043520115715No Hit
CCAGACGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC2720.13291569138149248No Hit
CCAGACGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCG2720.13291569138149248No Hit
CCAGACGGGGCTCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAG2710.13242703075141346No Hit
CCAGACGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAG2670.13047238823109739No Hit
CCAGACGGGATTTTTCTTCCTGTTCAGTGCTTTATCACCATCTGTTGTTTGT2650.12949506697093935No Hit
CCAGACGGGGCTCGTTCTCTTTCTGCCGCCATCTTGGTTCCGTGATCTCCGC2650.12949506697093935No Hit
CCAGACGGGATATGATTTTCCACTAATAAGCAAGCAATTAATTTTTTCTGAA2640.12900640634086033No Hit
CCAGACGGGGTGCTCCAGACGGGCTTTTTTTCGCCGCCGGACGCCGCCGAGG2630.1285177457107813No Hit
CCAGACGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGA2630.1285177457107813No Hit
CCAGACGGGAGCTTGTACATGAAACTTTGTATAAAAAAACAAAAACAAAACA2620.1280290850807023No Hit
CCAGACGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG2610.1275404244506233No Hit
CCAGACGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATG2570.12558578193030723No Hit
CCAGACGGGAGGGTGTCACGGCTGCTGTGCAATTATCTGAAGTTGCCTGACA2570.12558578193030723No Hit
CCAGACGGGTGGCATACGCGAAGCTGCCAAAAGCCTTAGACAAGCGCCAAGC2560.1250971213002282No Hit
CCAGACGGGGCTCCAGACGGTGTGTGTGGTTGCCACACAGCATTGTACCCAG2550.12460846067014919No Hit
CCAGACGGGTGCTAAGGTTGAGCGAGCTGACGGATATGAACCGCCAGTCCAA2550.12460846067014919No Hit
CCAGACGGGGAAAGTCTGGCGGTAGAGTGTCTGGGTGCTGGCTGACGTGCGG2550.12460846067014919No Hit
CCAGACGGGGACGGGATGTTCCATGCAAACTTCCGTTACTGTTGGCAAACTC2530.12363113940999115No Hit
CCAGACGGGAGCACCTTGGCCACCACTTCTCTGTGCCACCGTGTGTTTGGCA2500.12216515751975411No Hit
CCAGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA2490.12167649688967509No Hit
CCAGACGGAGCTCCCGCGGTTCCTCTCCCCACTGTGGCCGCGGCGCGGGAAG2480.12118783625959606No Hit
CCAGACGGGAACAGTGTTGGATAAAGCCCCCCACATCTGCATGGCTCCTCCC2430.118744533109201No Hit
CCAGACGGGGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCCCGGAAGAAT2430.118744533109201No Hit
CCAGACGGGATTGCTGTGAATTATATTGACAGTTGGTGAGCTGTCTTATATT2410.11776721184904296No Hit
CCAGACGGGGCTCCAGACGGGATGCAGTGCTCCAGACGGGGTTTTATCCAAC2410.11776721184904296No Hit
CCAGACGGGGCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGG2400.11727855121896394No Hit
CCAGACGGGCTTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGA2400.11727855121896394No Hit
CCAGACGGGGCTCCAGACGGGGGGCTGGTGAGATGGCTCAAAGGGTAAAAGC2390.11678989058888493No Hit
CCAGACGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATGGCTC2380.11630122995880592No Hit
CCAGACGGGGAGACATTGAAAGTGTATTAACCAGCATGAAAGATGTGTTTCG2370.11581256932872688No Hit
CCAGACGGGTCCACCACCCCGGCTGTGCTCTCCGACTGCCGCCGCCGCCGCC2360.11532390869864786No Hit
CCAGACGGGTACTCGGAAGGGTTGAGCGTCCACACTCACATCGTCAGCACCA2360.11532390869864786No Hit
CCAGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA2350.11483524806856886No Hit
CCAGACGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA2350.11483524806856886No Hit
CCAGACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT2340.11434658743848984No Hit
CCAGACGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC2330.11385792680841084No Hit
CCAGACGGGAGAGTCTGACCCCAGGCTGGGACAGGATTCCAGGCCCTCTTCC2330.11385792680841084No Hit
CCAGACGGGATTTTCTCCTTCCTTTTCCTCCCTTCTGCTCCACCTTCCCTTT2330.11385792680841084No Hit
CCAGACGGGAAGAACAACCTGAAGGACTGTGGCCTCTTCTGAGGGGCAGTGG2320.11336926617833182No Hit
CCAGACGGGGATGAGGACGAGGAGGAGAGTGAAAGCTCAGACTCTGAGGAGG2310.11288060554825279No Hit
CCAGACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC2300.11239194491817377No Hit
CCAGACGGGTGAGGTAAGTTCTTTAACGTTTCATTAAAAAACATTTTTTACA2290.11190328428809476No Hit
CCAGACGGGTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCT2280.11141462365801574No Hit
CCAGACGGGGAGGCGTGGAGGTTGGTGGCTGTTGAGCGGTTCCCGTGGTAAA2260.1104373023978577No Hit
CCAGACGGCAACTGTACGGGAGCTTAACTTTATAAGGAAATGTATTTTGACA2260.1104373023978577No Hit
CCAGACGGGGCTCCAGACGGGGACCCCAAAACAAGGCCCGCGAATGTTGGGA2240.10945998113769967No Hit
CCAGACGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA2240.10945998113769967No Hit
CCAGACGGGCTTTCCTTGGCGTGATTCCGTCCTGCGCGTCTGTTCTGTGGAA2240.10945998113769967No Hit
CCAGACGGGAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAA2240.10945998113769967No Hit
CCAGACGGGGCTCACATGGGTTATATGCTAAGAAGTATCCTGCATGTCATTG2220.10848265987754165No Hit
CCAGACGGGGGGATCTGCGGCGGCGACAGTTGGAGAGGGACGTTCCCGGGAA2190.10701667798730459No Hit
CCAGACGGGGCTTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCA2190.10701667798730459No Hit
CCAGACGGGCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGG2160.10555069609706755No Hit
CCAGACGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACCT2160.10555069609706755No Hit
CCAGACGGGGCTCCAGACGGGTAGCATTTTTTTTGTCTTTGTAATTTACAGA2160.10555069609706755No Hit
CCAGACGGGAGATCATGAAGGTGGAAGAGATTGCAGCTGGCAAGTGCCGCCG2150.10506203546698854No Hit
CCAGACGGGTGCTGAGCAACACCACAGCCATTGCTGAGGCCTGGGCTCGCCT2140.10457337483690951No Hit
CCAGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC2120.10359605357675149No Hit
CCAGACGGGATATGCTCTCTGCGTCTCAGTGTGTTCTAACTGATCACATTCC2110.10310739294667247No Hit
CCAGACGGGCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACAA2100.10261873231659346No Hit
CCAGACGGGCTTTTTCCCCCTTAGCGCGGCCGACTCTGCAGGTCTGCGTGGA2100.10261873231659346No Hit
CCAGACGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAAGCC2100.10261873231659346No Hit
CCAGACGGGGTTTTACGGCATCATGGCTGCCCTCCGGCCTCTGGTGAAGCCC2100.10261873231659346No Hit
CCAGACGGGAACTGACCTGTTTCTAAATGAAAGAGATGTGGGAGAGTGCTCT2100.10261873231659346No Hit
CCAGACGGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACCA2100.10261873231659346No Hit
CCAGACGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGG2090.10213007168651442No Hit
CCAGACGGGGTGATCATCTAGGACCAGGGCGACCGGGGCGCGCGATCCTTTC2090.10213007168651442No Hit
CCAGACGGGCCTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACA2080.10164141105643541No Hit
CCAGACGGGTCAAGACACTTCTTCAGACTGCTGCTTCTCTTATGCCACACAG2060.10066408979627739No Hit
CCAGACGGGGCCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA2060.10066408979627739No Hit
CCAGACGGAGAGTGAGTGAGTGTGTATGTGTGTGTGTGTGTGTCAGACTACC2050.10017542916619837No Hit
CCAGACGGGATCTACCGCATGATTAAACTAGGCCTGGGCATCGATGAAGATG2050.10017542916619837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT206.299236E-446.00000433
TTGATGG405.5733835E-946.00000436
GCCCCGG750.046.00000419
CATGGTT206.299236E-446.00000428
GCCCCCC206.299236E-446.00000426
TTTAGCG206.299236E-446.00000422
GTCATAA206.299236E-446.00000432
TATCAGG206.299236E-446.00000427
TTCTAGC206.299236E-446.00000416
CGGAATT405.5733835E-946.00000433
CGGAATC405.5733835E-946.00000422
TGTCACG405.5733835E-946.00000414
CGGGTCA800.046.0000046
AAGTAGG206.299236E-446.00000437
GGAATGG351.0140684E-746.00000432
AGCGTCA405.5733835E-946.00000441
GGAATAG206.299236E-446.00000423
AACCGGA405.5733835E-946.00000430
TTGGCCG750.046.00000437
ACAAATC206.299236E-446.00000425
Produced by FastQC (version 0.11.5)